SIDT1 (NM_017699) Human Untagged Clone

CAT#: SC316839

SIDT1 (untagged)-Human SID1 transmembrane family, member 1 (SIDT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIDT1
• Synonyms: SID-1; SID1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_017699 edited
  ATGCGCGGCTGCCTGCGGCTCGCGCTGCTCTGCGCGCTGCCCTGGCTCCTGCTGGCGGCG
  TCGCCCGGGCACCCGGCGAAATCCCCCAGGCAGCCCCCGGCACCGCGCCGCGACCCCTTC
  GACGCTGCCAGGGGCGCCGATTTCGATCATGTCTACAGCGGGGTGGTGAACCTCAGCACC
  GAGAACATCTACTCTTTCAACTACACCAGCCAGCCCGACCAGGTGACAGCCGTGAGGGTG
  TATGTGAACAGTTCCTCTGAGAATCTCAACTACCCGGTCCTTGTTGTGGTTCGCCAGCAG
  AAAGAGGTGCTGTCCTGGCAGGTTCCTCTGCTCTTCCAAGGACTATACCAGAGGAGCTAC
  AACTATCAAGAAGTGAGCCGCACCTTATGTCCCTCAGAAGCAACCAATGAGACGGGACCC
  TTGCAGCAACTGATATTTGTAGATGTCGCATCCATGGCACCCCTGGGTGCTCAGTACAAA
  CTGCTAGTTACCAAGCTGAAGCACTTCCAGCTCCGGACAAATGTTGCCTTTCACTTTACT
  GCCAGCCCCTCTCAACCTCAGTATTTTCTATACAAGTTTCCCAAAGACGTGGACTCAGTT
  ATCATTAAAGTGGTGTCTGAAATGGCTTATCCATGTTCTGTTGTCTCAGTCCAGAATATC
  ATGTGCCCGGTGTATGATCTCGACCACAATGTGGAATTTAATGGTGTCTATCAGTCCATG
  ACCAAGAAAGCTGCCATCACGCTACAGAAGAAGGATTTTCCAGGCGAGCAGTTCTTCGTG
  GTATTTGTGATAAAGCCTGAAGATTATGCCTGTGGAGGATCTTTCTTCATCCAGGAAAAG
  GAAAACCAGACCTGGAATCTACAGCGAAAAAAGAACCTTGAAGTGACCATTGTCCCTTCC
  ATTAAAGAATCTGTTTATGTGAAATCCAGTCTTTTCAGTGTCTTCATCTTCCTGTCCTTC
  TACTTGGGATGCCTTCTTGTTGGGTTTGTTCATTATCTGAGGTTTCAGAGAAAATCCATT
  GATGGAAGCTTTGGGTCCAATGATGGCTCTGGAAATATGGTGGCATCTCATCCCATTGCT
  GCCAGCACACCCGAAGGGAGCAATTATGGGACAATAGATGAGTCAAGCTCCAGTCCTGGA
  AGGCAGATGTCCTCCTCCGATGGTGGGCCACCGGGCCAGTCAGACACAGACAGCTCCGTG
  GAGGAGAGCGACTTCGACACCATGCCAGACATTGAGAGTGATAAAAACATCATCCGGACC
  AAGATGTTCCTTTACCTGTCAGATTTGTCCAGGAAGGACCGGAGAATTGTCAGCAAAAAA
  TATAAAATTTATTTTTGGAACATCATCACCATTGCTGTGTTTTACGCGCTGCCCGTGATC
  CAGCTGGTCATTACCTATCAGACAGTGGTAAATGTCACTGGCAACCAGGACATCTGTTAC
  TACAACTTCCTCTGTGCTCACCCCTTGGGCGTCCTGAGTGCCTTCAACAACATTCTCAGC
  AATCTGGGCCACGTGCTTCTGGGCTTCCTCTTCCTGCTGATAGTCTTGCGCCGCGACATC
  CTCCATCGGAGAGCCCTGGAAGCCAAGGACATCTTTGCTGTGGAGTACGGGATTCCCAAA
  CACTTTGGTCTCTTCTACGCTATGGGCATTGCATTGATGATGGAAGGGGTGCTCAGTGCT
  TGCTACCATGTCTGCCCTAATTATTCCAACTTCCAATTCGACACCTCCTTCATGTACATG
  ATCGCTGGCCTGTGCATGCTGAAGCTCTATCAGACCCGCCACCCAGACATCAATGCCAGC
  GCCTACTCTGCCTATGCCTCCTTTGCTGTGGTCATCATGGTCACCGTCCTTGGAGTGGTG
  TTTGGAAAAAATGACGTATGGTTCTGGGTCATCTTCTCTGCAATCCACGTTCTGGCCTCG
  CTAGCCCTCAGCACCCAGATATATTATATGGGTCGTTTCAAGATAGATTTGGGAATTTTC
  CGGCGGGCTGCCATGGTGTTCTACACAGACTGTATCCAGCAGTGTAGCCGACCTCTATAT
  ATGGATAGAATGGTGTTGCTGGTTGTGGGGAATCTGGTTAACTGGTCCTTCGCCCTCTTT
  GGATTGATATACCGCCCCAGGGACTTTGCTTCCTACATGCTGGGCATCTTCATCTGTAAC
  CTTTTGCTGTACCTGGCCTTTTACATCATCATGAAGCTCCGCAGCTCTGAAAAGGTCCTC
  CCAGTCCCGCTCTTCTGCATCGTGGCCACCGCTGTGATGTGGGCTGCCGCCCTATATTTT
  TTCTTCCAGAATCTCAGCAGCTGGGAGGGAACTCCGGCCGAATCCCGGGAGAAGAACCGC
  GAGTGCATTCTGCTGGATTTCTTCGATGACCATGACATCTGGCACTTCCTCTCTGCTACT
  GCTCTGTTTTTCTCATTCTTGGTTTTGTTAACTTTGGATGATGACCTTGATGTGGTTCGG
  AGAGACCAGATCCCTGTCTTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017699 unedited
  CGTCGGTCGTCGTGGATTGTCTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCA
  CGAGGAGACCGAATTTCCTCCTCGATGTGGCGTCAGGTTGACTTTTCGAGACTAGCGGGT
  ATTTCTTTTTAATGACTCCAATGCCTTTTTATTATTGCTGTTATTGAGGTTGAGGGAGAA
  GAGATCGGTCTAAATTCTGGCTGGGTAAGTGGGGGGATTCTCGGCGATGAGAAACGGGGG
  ACTTAGAAGCCGGAGGAAAATCAGCAGCCCCACATCTCCACTTCTCCAGTCCGCCCTACT
  CTCCACCCGTGACCTCCAGTGGAGACCCCAGGCGGCAGCATCAGTATTTGATCGGCCCTT
  CGTCAGCACGCTGCCAGCCCTGGCCGGCTGGGTTCGCCAGGCATCACCCGCTCGGCTCTG
  AAGCGGACGCCTGGCCCTGCACCGGGCTTTGGAAGGACCCTCTCTGCGCTCGCCCCCTCC
  CCAGGGTGGCTCCGCTTTCGAGCCCGGGCGCGGTGCCCACCATGCGCGGCTGCCTGCGGC
  TCGCGCTGCTCTGCGCGCTGCCCTGGCTCCTGCTGGCGGCGTCGCCCGGGCACCCGGCGA
  AATCCCCCAGGCAGCCCCCGGCACCGCGCCGCGACCCCTTCGACGCTGCCAGGGGCGCCG
  ATTTCGATCATGTCTACAGCGGGGTGGTGAACCTCAGCACCGAGAACATCTACTCTTTCA
  ACTACACCAGCCAGCCCGACCAGGTGACAGCCGTGAGGGTGTATGTGAACAGTTCCTCTG
  AGAATCTCAACTACCCGGTCCTTGTTGTGGTTCGCCAGCAGAAAGAGGTGCTGTCCTGGC
  AGTTTCCTCTGCTCTTCCAAGGACTATACCAGAGGAGCTACACTATCAAGAGTGAGCCGC
  ACCTTATGTCCCTCAGAAGCCACCAATGGAGACGGGACCCTTGCAGCAACCTGA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_017699 unedited
  CCATGCCTGAAAGGTGTTGCTGGTTGTGGGGAATCTTGGCTTAACTGGTCCTTCGCCCTC
  TTTGGATTGATATACCTGCCCCAGGGACTTTGCTTCCTACATGCTGGGCATCTTCATCTG
  TAACCTTTTGCTGTACCTGGCCTTTTACATCATCATGAAGCTCCGCAGCTCTGAAAAGGT
  CCTCCCAGTCCCGCTCTTCTGCATCGTGGCCACCGCTGTGATGTGGGCTGCCGCCCTATA
  TTTTTTCTTCCAGAATCTCAGCAGCTGGGAGGGAACTCCGGCCGAATCCCGGGAGAAGAA
  CCGCGAGTGCATTCTGCTGGATTTCTTCGATGACCATGACATCTGGCACTTCCTCTCTGC
  TACTGCTCTGTTTTTCTCATTCTTGGTTTTGTTAACTTTGGATGATGACCTTGATGTGGT
  TCGGAGAGACCAGATCCCTGTCTTCTGAACCTCCAACATTAAGAGAGGGGAGGGAGCGAT
  CAATCTTGGTGCTGTTTCACAAAAATTACAGTGACCACAGCAAAGTAACCACTGCCAGAT
  GCTCCACTCACCCTCTGTAGAGCCAACTCTGCATTCACACAGGAAGGAGAGGGGCTGCGG
  GAGATTTAAACCTGCAAGAAAGGAGGCAGAAGGGGAGCCATGTTTTGAGGACAGACGCAA
  ACCTGAGGAGCTGAGAAACACTTGCTCCTTCCATCTGCAGCTTTGGGAGTGCAACAGGGA
  TAGGCACTGCATCCAAGTCAACTCACCATCTTGGGGTCCCTCCCACCCTCACGGAGACTT
  GCCAGCAATGGCAGAATGCTGCTGCACACTTCCCTCCAGTTGTCACCCCTGCCCAGAAAG
  GCCAGCAGCTTGACTTCCTGCCCAGAAACTGTGTTGGCCCCCTTCACACCTCTGCACACC
  TGCTGCTCAGCAGAGATGTGATTCTTTAGAAATATGGCGGGGGAGGGTGACCCCAGGCCC
  TGCCCTACTGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_017699
• Insert Size: 4800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to have a single amino acid difference from the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017699.2, NP_060169.2
• RefSeq Size: 5021 bp
• RefSeq ORF: 2484 bp
• Locus ID: 54847
• UniProt ID: Q9NXL6
• Cytogenetics: 3q13.2
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene belongs to SID1 family of transmembrane dsRNA-gated channels. Family members transport dsRNA into cells and are required for systemic RNA interference. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (2) encodes isoform 2.