DLL1 (NM_005618) Human Untagged Clone

CAT#: SC316255

DLL1 (untagged)-Human delta-like 1 (Drosophila) (DLL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DLL1
• Synonyms: Delta; DELTA1; DL1; NEDBAS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005618 edited
  GAATTCGGCACGAGGGCCCATCGATCCCCCGCGCGTCCGCCGCTGTTCTAAGGAGAGAAG
  TGGGGGCCCCCCAGGCTCGCGCGTGGAGCGAAGCAGCATGGGCAGTCGGTGCGCGCTGGC
  CCTGGCGGTGCTCTCGGCCTTGCTGTGTCAGGTCTGGAGCTCTGGGGTGTTCGAACTGAA
  GCTGCAGGAGTTCGTCAACAAGAAGGGGCTGCTGGGGAACCGCAACTGCTGCCGCGGGGG
  CGCGGGGCCACCGCCGTGCGCCTGCCGGACCTTCTTCCGCGTGTGCCTCAAGCACTACCA
  GGCCAGCGTGTCCCCCGAGCCGCCCTGCACCTACGGCAGCGCCGTCACCCCCGTGCTGGG
  CGTCGACTCCTTCAGTCTGCCCGACGGCGGGGGCGCCGACTCCGCGTTCAGCAACCCCAT
  CCGCTTCCCCTTCGGCTTCACCTGGCCGGGCACCTTCTCTCTGATTATTGAAGCTCTCCA
  CACAGATTCTCCTGATGACCTCGCAACAGAAAACCCAGAAAGACTCATCAGCCGCCTGGC
  CACCCAGAGGCACCTGACGGTGGGCGAGGAGTGGTCCCAGGACCTGCACAGCAGCGGCCG
  CACGGACCTCAAGTACTCCTACCGCTTCGTGTGTGACGAACACTACTACGGAGAGGGCTG
  CTCCGTTTTCTGCCGTCCCCGGGACGATGCCTTCGGCCACTTCACCTGTGGGGAGCGTGG
  GGAGAAAGTGTGCAACCCTGGCTGGAAAGGGCCCTACTGCACAGAGCCGATCTGCCTGCC
  TGGATGTGATGAGCAGCATGGATTTTGTGACAAACCAGGGGAATGCAAGTGCAGAGTGGG
  CTGGCAGGGCCGGTACTGTGACGAGTGTATCCGCTATCCAGGCTGTCTCCATGGCACCTG
  CCAGCAGCCCTGGCAGTGCAACTGCCAGGAAGGCTGGGGGGGCCTTTTCTGCAACCAGGA
  CCTGAACTACTGCACACACCATAAGCCCTGCAAGAATGGAGCCACCTGCACCAACACGGG
  CCAGGGGAGCTACACTTGCTCTTGCCGGCCTGGGTACACAGGTGCCACCTGCGAGCTGGG
  GATTGACGAGTGTGACCCCAGCCCTTGTAAGAACGGAGGGAGCTGCACGGATCTCGAGAA
  CAGCTACTCCTGTACCTGCCCACCCGGCTTCTACGGCAAAATCTGTGAATTGAGTGCCAT
  GACCTGTGCGGACGGCCCTTGCTTTAACGGGGGTCGGTGCTCAGACAGCCCCGATGGAGG
  GTACAGCTGCCGCTGCCCCGTGGGCTACTCCGGCTTCAACTGTGAGAAGAAAATTGACTA
  CTGCAGCTCTTCACCCTGTTCTAATGGTGCCAAGTGTGTGGACCTCGGTGATGCCTACCT
  GTGCCGCTGCCAGGCCGGCTTCTCGGGGAGGCACTGTGACGACAACGTGGACGACTGCGC
  CTCCTCCCCGTGCGCCAACGGGGGCACCTGCCGGGATGGCGTGAACGACTTCTCCTGCAC
  CTGCCCGCCTGGCTACACAGGCAGGAACTGCAGTGCCCCCGTCAGCAGGTGCGAGCACGC
  ACCCTGCCACAATGGGGCCACCTGCCACGAGAGGGGCCACCGCTATGTGTGCGAGTGTGC
  CCGAGGCTACGGGGGTCCCAACTGCCAGTTCCTGCTCCCCGAGCTGCCCCCGGGCCCAGC
  GGTGGTGGACCTCACTGAGAAGCTAGAGGGCCAGGGCGGGCCATTCCCCTGGGTGGCCGT
  GTGCGCCGGGGTCATCCTTGTCCTCATGCTGCTGCTGGGCTGTGCCGCTGTGGTGGTCTG
  CGTCCGGCTGAGGCTGCAGAAGCACCGGCCCCCAGCCGACCCCTGCCGGGGGGAGACGGA
  GACCATGAACAACCTGGCCAACTGCCAGCGTGAGAAGGACATCTCAGTCAGCATCATCGG
  GGCCACGCAGATCAAGAACACCAACAAGAAGGCGGACTTCCACGGGGACCACAGCGCCGA
  CAAGAATGGCTTCAAGGCCCGCTACCCAGCGGTGGACTATAACCTCGTGCAGGACCTCAA
  GGGTGACGACACCGCCGTCAGGGACGCGCACAGCAAGCGTGACACCAAGTGCCAGCCCCA
  GGGCTCCTCAGGGGAGGAGAAGGGGACCCCGACCACACTCAGGGGTGGAGAAGCATCTGA
  AAGAAAAAGGCCGGACTCGGGCTGTTCAACTTCAAAAGACACCAAGTACCAGTCGGTGTA
  CGTCATATCCGAGGAGAAGGATGAGTGCGTCATAGCAACTGAGGTGTAAAATGGAAGTGA
  GATGGCAAGACTCCCGTTTCTCTTAAAATAAGTAAAATTCCAAGGATATATGCCCCAACG
  AATGCTGCTGAAGAGGAGGGAGGCCTCGTGGACTGCTGCTGAGAAACCGAGTTCAGACCG
  AGCAGGTTCTCCTCCTGAGGTCCTCGACGCCTGCCGACAGCCTGTCGCGGCCCGGCCGCC
  TGCGGCACTGCCTTCCGTGACGTCGCCGTTGCACTATGGACAGTTGCTCTTAAGAGAATA
  TATATTTAAATGGGTGAACTGAATTACGCATAAGAAGCATGCACTGCCTGAGTGTATATT
  TTGGATTCTTATGAGCCAGTCTTTTCTTGAATTAGAAACACAAACACTGCCTTTATTGTC
  CTTTTTGATACGAAGATGTGCTTTTTCTAGATGGAAAAGATGTGTGTTATTTTTTGGATT
  
• Restriction Sites: Please inquire
• ACCN: NM_005618
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005618.3, NP_005609.3
• RefSeq Size: 3366 bp
• RefSeq ORF: 2172 bp
• Locus ID: 28514
• UniProt ID: O00548
• Cytogenetics: 6q27
• Domains: DSL, EGF_CA, EGF, EGF
• Protein Families: Adult stem cells, Cancer stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Notch signaling pathway
• Gene Summary: DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]