5 Lipoxygenase (ALOX5) (NM_000698) Human Untagged Clone

CAT#: SC316253

ALOX5 (untagged)-Human arachidonate 5-lipoxygenase (ALOX5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 5 Lipoxygenase
• Synonyms: 5-LO; 5-LOX; 5LPG; LOG5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000698 edited
  CTGCGGCGCTAGATGCGGACACCTGGACCGCCGCGCCGAGGCTCCCGGCGCTCGCTGCTC
  CCGCGGCCCGCGCCATGCCCTCCTACACGGTCACCGTGGCCACTGGCAGCCAGTGGTTCG
  CCGGCACTGACGACTACATCTACCTCAGCCTCGTGGGCTCGGCGGGCTGCAGCGAGAAGC
  ACCTGCTGGACAAGCCCTTCTACAACGACTTCGAGCGTGGCGCGGTGGATTCATACGACG
  TGACTGTGGACGAGGAACTGGGCGAGATCCAGCTGGTCAGAATCGAGAAGCGCAAGTACT
  GGCTGAATGACGACTGGTACCTGAAGTACATCACGCTGAAGACGCCCCACGGGGACTACA
  TCGAGTTCCCCTGCTACCGCTGGATCACCGGCGATGTCGAGGTTGTCCTGAGGGATGGAC
  GCGCAAAGTTGGCCCGAGATGACCAAATTCACATTCTCAAGCAACACCGACGTAAAGAAC
  TGGAAACACGGCAAAAACAATATCGATGGATGGAGTGGAACCCTGGCTTCCCCTTGAGCA
  TCGATGCCAAATGCCACAAGGATTTACCCCGTGATATCCAGTTTGATAGTGAAAAAGGAG
  TGGACTTTGTTCTGAATTACTCCAAAGCGATGGAGAACCTGTTCATCAACCGCTTCATGC
  ACATGTTCCAGTCTTCTTGGAATGACTTCGCCGACTTTGAGAAAATCTTTGTCAAGATCA
  GCAACACTATTTCTGAGCGGGTCATGAATCACTGGCAGGAAGACCTGATGTTTGGCTACC
  AGTTCCTGAATGGCTGCAACCCTGTGTTGATCCGGCGCTGCACAGAGCTGCCCGAGAAGC
  TCCCGGTGACCACGGAGATGGTAGAGTGCAGCCTGGAGCGGCAGCTCAGCTTGGAGCAGG
  AGGTCCAGCAAGGGAACATTTTCATCGTGGACTTTGAGCTGCTGGATGGCATCGATGCCA
  ACAAAACAGACCCCTGCACACTCCAGTTCCTGGCCGCTCCCATCTGCTTGCTGTATAAGA
  ACCTGGCCAACAAGATTGTCCCCATTGCCATCCAGCTCAACCAAATCCCGGGAGATGAGA
  ACCCTATTTTCCTCCCTTCGGATGCAAAATACGACTGGCTTTTGGCCAAAATCTGGGTGC
  GTTCCAGTGACTTCCACGTCCACCAGACCATCACCCACCTTCTGCGAACACATCTGGTGT
  CTGAGGTTTTTGGCATTGCAATGTACCGCCAGCTGCCTGCTGTGCACCCCATTTTCAAGC
  TGCTGGTGGCACACGTGAGATTCACCATTGCAATCAACACCAAGGCCCGTGAGCAGCTCA
  TCTGCGAGTGTGGCCTCTTTGACAAGGCCAACGCCACAGGGGGCGGTGGGCACGTGCAGA
  TGGTGCAGAGGGCCATGAAGGACCTGACCTATGCCTCCCTGTGCTTTCCCGAGGCCATCA
  AGGCCCGGGGCATGGAGAGCAAAGAAGACATCCCCTACTACTTCTACCGGGACGACGGGC
  TCCTGGTGTGGGAAGCCATCAGGACGTTCACGGCCGAGGTGGTAGACATCTACTACGAGG
  GCGACCAGGTGGTGGAGGAGGACCCGGAGCTGCAGGACTTCGTGAACGATGTCTACGTGT
  ACGGCATGCGGGGCCGCAAGTCCTCAGGCTTCCCCAAGTCGGTCAAGAGCCGGGAGCAGC
  TGTCGGAGTACCTGACCGTGGTGATCTTCACCGCCTCCGCCCAGCACGCCGCGGTCAACT
  TCGGCCAGTACGACTGGTGCTCCTGGATCCCCAATGCGCCCCCAACCATGCGAGCCCCGC
  CGCCGACTGCCAAGGGCGTGGTGACCATTGAGCAGATCGTGGACACGCTGCCCGACCGCG
  GCCGCTCCTGCTGGCATCTGGGTGCAGTGTGGGCGCTGAGCCAGTTCCAGGAAAACGAGC
  TGTTCCTGGGCATGTACCCAGAAGAGCATTTTATCGAGAAGCCTGTGAAGGAAGCCATGG
  CCCGATTCCGCAAGAACCTCGAGGCCATTGTCAGCGTGATTGCTGAGCGCAACAAGAAGA
  AGCAGCTGCCATATTACTACTTGTCCCCAGACCGGATTCCGAACAGTGTGGCCATCTGAG
  CACACTGCCAGTCTCACTGTGGGAAGGCCAGCTGCCCCAGCCAGATGGACTCCAGCCTGC
  CTGGCAGGCTGTCTGGCCAGGCCTCTTGGCAGTCACATCTCTTCCTCCGAGGCCAGTACC
  TTTCCATTTATTCTTTGATCTTCAGGGAACTGCATAGATTGATCAAAGTGTAAACACCAT
  AGGGACCCATTCTACACAGAGCAGGACTGCACAGCGTCCTGTCCACACCCAGCTCAGCAT
  TTCCACACCAAGCAGCAACAGCAAATCACGACCACTGATAGATGTCTATTCTTGTTGGAG
  ACATGGGATGATTATTTTCTGTTCTATTTGTGCTTAGTCCAATTCCTTGCACATAGTAGG
  TACCCAATTCAATTACTATTGAATGAATTAAGAATTGGTTGCCATAAAAATAAATCAGTT
  CATTTAAACAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000698 unedited
  NGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGCTGCGGC
  GCTAGATGCGGACACCTGGACCGCCGCGCCGAGGCTCCCGGCGCTCGCTGCTCCCGCGGC
  CCGCGCCATGCCCTCCTACACGGTCACCGTGGCCACTGGCAGCCAGTGGTTCGCCGGCAC
  TGACGACTACATCTACCTCAGCCTCGTGGGCTCGGCGGGCTGCAGCGAGAAGCACCTGCT
  GGACAAGCCCTTCTACAACGACTTCGAGCGTGGCGCGGTGGATTCATACGACGTGACTGT
  GGACGAGGAACTGGGCGAGATCCAGCTGGTCAGAATCGAGAAGCGCAAGTACTGGCTGAA
  TGACGACTGGTACCTGAAGTACATCACGCTGAAGACGCCCCACGGGGACTACATCGAGTT
  CCCCTGCTACCGCTGGATCACCGGCGATGTCGAGGTTGTCCTGAGGGATGGACGCGCAAA
  GTTGGCCCGAGATGACCAAATTCACATTCTCAAGCAACACCGACGTAAAGAACTGGAAAC
  ACGGCAAAAACAATATCGATGGATGGAGTGGAACCCTGGCTTCCCCTTGAGCATCGATGC
  CAAATGCCACAAGGATTTACCCCGTGATATCCAGTTTGATAGTGAAAAAGGAGTGGACTT
  TGTTCTGAATTACTCCAAAGCGATGGAGAACCTGTTCATCAACCGCTTCATGCACATGTT
  CCAGTCTTCTTGGAATGACTTCGCCGACTTTGAGAAAATCTTTGTCAAGATCAGCAACAC
  TATTTCTGAGCGGGTCATGAATCACTGGCAGGAAGACCTGATGTTTGGCTACCAGTTCCT
  GAATGGGCTGCACCCCCTGTGTGATCCGGCGCTGCACAGAGCTGCCCGAGAGCTCCGGTG
  ACCACGNAGATGGTAAAGTGCANCCTGGAGCGGCAGCTCAACTTGGAGCCAGAGGTCCGC
  AN
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000698 unedited
  CTTTTGCCACTCCGAGGCCTCAAGGCCCGGGGCACTGAGAGCAAAGAAGACATTCCCCTT
  ACTACTCCTACCGGGACGACGGGCTCCTGGTGTGGGAAGCCATCAGGACGTTCACGGCCG
  AGGTGGTAGACATCTACTACGAGGGCGACCAGGTGGTGGAGGAGGACCCGGAGCTGCAGG
  ACTTCGTGAACGATGTCTACGTGTACGGCATGCGGGGCCGCAAGTCCTCAGGCTTCCCCA
  AGTCGGTCAAGAGCCGGGAGCAGCTGTCGGAGTACCTGACCGTGGTGATCTTCACCGCCT
  CCGCCCAGCACGCCGCGGTCAACTTCGGCCAGTACGACTGGTGCTCCTGGATCCCCAATG
  CGCCCCCAACCATGCGAGCCCCGCCGCCGACTGCCAAGGGCGTGGTGACCATTGAGCAGA
  TCGTGGACACGCTGCCCGACCGCGGCCGCTCCTGCTGGCATCTGGGTGCAGTGTGGGCGC
  TGAGCCAGTTCCAGGAAAACGAGCTGTTCCTGGGCATGTACCCAGAAGAGCATTTTATCG
  AGAAGCCTGTGAAGGAAGCCATGGCCCGATTCCGCAAGAACCTCGAGGCCATTGTCAGCG
  TGATTGCTGAGCGCAACAAGAAGAAGCAGCTGCCATATTACTACTTGTCCCCAGACCGGA
  TTCCGAACAGTGTGGCCATCTGAGCACACTGCCAGTCTCACTGTGGGAAGGCCAGCTGCC
  CCAGCCAGATGGACTCCAGCCTGCCTGGCAGCTGTCTGGCCAGGCCTCTTGGCAGTCACA
  TCTCTTCCTCCGAGGCCAGTACCTTTCCATTTATTCTTTGATCTTCAGGGAACTGCATAG
  ATTGATCAAAGTGTAAACACCATAGGGACCCATTCTACACAGAGCAGACTGCACAGCGTC
  CTGTCCACACCCAGCTCAGCATTTCCACACCAGCAGCACAGCAATCACGACCACTGATAG
  ATGTCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000698
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000698.2, NP_000689.1
• RefSeq Size: 2568 bp
• RefSeq ORF: 2025 bp
• Locus ID: 240
• UniProt ID: P09917
• Cytogenetics: 10q11.21
• Domains: lipoxygenase, PLAT
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1).