RPS6KA3 (NM_004586) Human Untagged Clone

CAT#: SC314588

RPS6KA3 (untagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KA3
• Synonyms: CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19; p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004586, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAGCCCGTCCGACAGCGCTGAGA
  ATGGACAGCAAATTATGGATGAACCTATGGGAGAGGAGGAGATTAACCCACAAACTGAAGAAGTCAGTAT
  CAAAGAAATTGCAATCACACATCATGTAAAGGAAGGACATGAAAAGGCAGATCCTTCCCAGTTTGAACTT
  TTAAAAGTATTAGGGCAGGGATCATTTGGAAAGGTTTTCTTAGTTAAAAAAATCTCAGGCTCTGATGCTA
  GGCAGCTTTATGCCATGAAGGTATTGAAGAAGGCCACACTGAAAGTTCGAGACCGAGTTCGGACAAAAAT
  GGAACGTGATATCTTGGTAGAGGTTAATCATCCTTTTATTGTCAAGTTGCATTATGCTTTTCAAACTGAA
  GGGAAGTTGTATCTTATTTTGGATTTTCTCAGGGGAGGAGATTTGTTTACACGCTTATCCAAAGAGGTGA
  TGTTCACAGAAGAAGATGTCAAATTCTACTTGGCTGAACTTGCACTTGCTTTAGACCATCTACATAGCCT
  GGGAATAATTTATAGAGACTTAAAACCAGAAAATATACTTCTTGATGAAGAAGGTCACATCAAGTTAACA
  GATTTCGGCCTAAGTAAAGAGTCTATTGACCATGAAAAGAAGGCATATTCTTTTTGTGGAACTGTGGAGT
  ATATGGCTCCAGAAGTAGTTAATCGTCGAGGTCATACTCAGAGTGCTGACTGGTGGTCTTTTGGTGTGTT
  AATGTTTGAAATGCTTACTGGTACACTCCCTTTCCAAGGAAAAGATCGAAAAGAAACAATGACTATGATT
  CTTAAAGCCAAACTTGGAATGCCACAGTTTTTGAGTCCTGAAGCGCAGAGTCTTTTACGAATGCTTTTCA
  AGCGAAATCCTGCAAACAGATTAGGTGCAGGACCAGATGGAGTTGAAGAAATTAAAAGACATTCATTTTT
  CTCAACGATAGACTGGAATAAACTGTATAGAAGAGAAATTCATCCGCCATTTAAACCTGCAACGGGCAGG
  CCTGAAGATACATTCTATTTTGATCCTGAGTTTACTGCAAAAACTCCCAAAGATTCACCTGGCATTCCAC
  CTAGTGCTAATGCACATCAGCTTTTTCGGGGGTTTAGTTTTGTTGCTATTACCTCAGATGATGAAAGCCA
  AGCTATGCAGACAGTTGGTGTACATTCAATTGTTCAGCAGTTACACAGGAACAGTATTCAGTTTACTGAT
  GGATATGAAGTAAAAGAAGATATTGGAGTTGGCTCCTACTCTGTTTGCAAGAGATGTATACATAAAGCTA
  CAAACATGGAGTTTGCAGTGAAGATTATTGATAAAAGCAAGAGAGACCCAACAGAAGAAATTGAAATTCT
  TCTTCGTTATGGACAGCATCCAAACATTATCACTCTAAAGGATGTATATGATGATGGAAAGTATGTGTAT
  GTAGTAACAGAACTTATGAAAGGAGGTGAATTGCTGGATAAAATTCTTAGACAAAAATTTTTCTCTGAAC
  GAGAGGCCAGTGCTGTCCTGTTCACTATAACTAAAACCGTTGAATATCTTCACGCACAAGGGGTGGTTCA
  TAGAGACTTGAAACCTAGCAACATTCTTTATGTGGATGAATCTGGTAATCCGGAATCTATTCGAATTTGT
  GATTTTGGCTTTGCAAAACAGCTGAGAGCGGAAAATGGTCTTCTCATGACTCCTTGTTACACTGCAAATT
  TTGTTGCACCAGAGGTTTTAAAAAGACAAGGCTATGATGCTGCTTGTGATATATGGAGTCTTGGTGTCCT
  ACTCTATACAATGCTTACCGGTTACACTCCATTTGCAAATGGTCCTGATGATACACCAGAGGAAATATTG
  GCACGAATAGGTAGCGGAAAATTCTCACTCAGTGGTGGTTACTGGAATTCTGTTTCAGACACAGCAAAGG
  ACCTGGTGTCAAAGATGCTTCATGTAGACCCTCATCAGAGACTGACTGCTGCTCTTGTGCTCAGACATCC
  TTGGATCGTCCACTGGGACCAACTGCCACAATACCAACTAAACAGACAGGATGCACCACATCTAGTAAAG
  GGTGCCATGGCAGCTACATATTCTGCTTTGAACCGTAATCAGTCACCAGTTTTGGAACCAGTAGGCCGCT
  CTACTCTTGCTCAGCGGAGAGGTATTAAAAAAATCACCTCAACAGCCCTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004586 unedited
  TATAACCCCCGCCCGTTGGCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGC
  GAATTCGGCACGAGGGAAGATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGC
  TGTGGAGAGCCCGTCCGACAGCGCTGAGAATGGACAGCAAATTATGGATGAACCTATGGG
  AGAGGAGGAGATTAACCCACAAACTGAAGAAGTCAGTATCAAAGAAATTGCAATCACACA
  TCATGTAAAGGAAGGACATGAAAAGGCAGATCCTTCCCAGTTTGAACTTTTAAAAGTATT
  AGGGCAGGGATCATTTGGAAAGGTTTTCTTAGTTAAAAAAATCTCAGGCTCTGATGCTAG
  GCAGCTTTATGCCATGAAGGTATTGAAGAAGGCCACACTGAAAGTTCGAGACCGAGTTCG
  GACAAAAATGGAACGTGATATCTTGGTAGAGGTTAATCATCCTTTTATTGTCAAGTTGCA
  TTATGCTTTTCAAACTGAAGGGAAGTTGTATCTTATTTTGGATTTTCTCAGGGGAGGAGA
  TTTGTTTACACGCCTATCCAAAGAGGTGATGTTCACAGAAGAAGATGTGCAATTCTACTT
  GGCTGAACTTGCGCTTGGCTTAGACCATCTACATAGCCTGGGGAATAATTTATAGAGACT
  TAAAACCCGAAAATATACTTTTTTGAGAAGAAAGGCCCCATCAGGTTAACAGAATTTCGG
  CCTAAGTAAAGAGTCTTTTGACCTTGAAAAGAAGGGGTATTCTTTTTGGGGAAGTGGGGA
  GTGTTTGGCTGCCGAAGATATTAATCGTCCAGGCCTCTCTCAAAGGCGGACGGGGGGGCT
  TTGGGGGGGTAAAGTTGGAAAGGTGCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004586 unedited
  NAATTTACTTTACCGCGCCCGTTTCTATGATCGATTTTTCTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTAACTATTTAACCAGTTTTAATAGTCAATTGATTAATAATAATAGATTCCA
  AGTTAAACAAGGCATGTATACATTAAAGAAAGCAGTACAGAAATGTACTGTATATGAACT
  GTTTACAAAAACATACAAAATGTTGGATGGTTTTTTGGATACAGTGCTAATATAAACGGA
  TTGTTTAAGCTAAGTGCTTAACATAAACTGTCCATCCCTATAACTAACAAAGAATATTTA
  AGGGACATGAAATATTTCCTATAAAAATAATGCTATATGGGGTACTTTATTCAGTGTATT
  TTTAGGGGGTATTCATATGTTCATTATTTTTCTCATTGATTCAGGGACTATATCTTCATC
  ATGTTTCCATTTTCATTTCTTCCGAAGCTCCAGGAAAATCTAACTTGCTAACAATCATTT
  AAAGCGAGAAGAGAGGAAAGCAGGAGCAGCAGCAGGAACAGCAGCATTATGGGTACCAGC
  TGGGACAGTGTGTGCTTGCAGGTGTCTCTCAAATACGTGCTACCTGTCGCCAGAACTTGT
  GCTATCAGCTTACACCATGGTACCAAATATCTCACTGAGGTCACTTCACAGGGCTGTTGA
  GGTGATTTTTTTAATACCTCTCCGCTGAGCAAGAGTAGAGCGGCCTACTGGTTCCAAAAC
  TGGTGACTGATTACGGTTCAAAGCAGAATATGTAGCTGCCATGGCCCCCTTTACTAGATG
  TGGTGCATCCTGTCTGTTTAGTTGGTATTGTGGCAATTTGTCCCCAGTGGACGATCCCAG
  GATGTCTGAACACAAAGACAACAGTCAATCTCTGATGAGGTTTTAATGAAACATTTTTGA
  AACCCGGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004586
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004586.2, NP_004577.1
• RefSeq Size: 7723 bp
• RefSeq ORF: 2223 bp
• Locus ID: 6197
• UniProt ID: P51812
• Cytogenetics: Xp22.12
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]