hnRNP L (HNRNPL) (NM_001533) Human Untagged Clone

CAT#: SC314118

HNRNPL (untagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: hnRNP L
• Synonyms: hnRNP-L; HNRPL; P/OKcl.14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001533 edited
  TCTGCGCCGCCATGTCGCGGAGGCTGCTGCCCCGGGCGGAGAAGCGGCGTCGGCGGCTGG
  AGCAGAGGCAGCAGCCGGACGAGCAGCGGAGGCGGTCGGGAGCGATGGTGAAGATGGCGG
  CGGCGGGCGGCGGAGGCGGCGGTGGCCGCTACTACGGCGGCGGCAGTGAGGGCGGCCGGG
  CCCCTAAGCGGCTCAAGACTGACAACGCCGGCGACCAGCACGGAGGCGGCGGCGGTGGCG
  GTGGAGGAGCCGGGGCGGCGGGCGGCGGCGGCGGTGGGGAGAACTACGATGACCCGCACA
  AAACCCCTGCCTCCCCAGTTGTCCACATCAGGGGCCTGATTGACGGTGTGGTGGAAGCAG
  ACCTTGTGGAGGCCTTGCAGGAGTTTGGACCCATCAGCTATGTGGTGGTAATGCCTAAAA
  AGAGACAAGCACTGGTGGAGTTTGAAGATGTGTTGGGGGCTTGCAACGCAGTGAACTACG
  CAGCCGACAACCAAATATACATTGCTGGTCACCCAGCTTTTGTCAACTACTCTACCAGCC
  AGAAGATCTCCCGCCCTGGGGACTCGGATGACTCCCGGAGCGTGAACAGTGTGCTTCTCT
  TTACCATCCTGAACCCCATTTATTCGATCACCACGGATGTTCTTTACACTATCTGTAATC
  CTTGTGGCCCTGTCCAGAGAATTGTCATTTTCAGGAAGAATGGAGTTCAGGCGATGGTGG
  AATTTGACTCAGTTCAAAGTGCCCAGCGGGCCAAGGCCTCTCTCAATGGGGCTGATATCT
  ATTCTGGCTGTTGCACTCTGAAGATCGAATACGCAAAGCCTACACGCTTGAATGTGTTCA
  AGAATGATCAGGATACTTGGGACTACACAAACCCCAATCTCAGTGGACAAGGTGACCCTG
  GCAGCAACCCCAACAAACGCCAGAGGCAGCCCCCTCTCCTGGGAGATCACCCCGCAGAAT
  ATGGAGGGCCCCACGGTGGGTACCACAGCCATTACCATGATGAGGGCTACGGGCCCCCCC
  CACCTCACTACGAAGGGAGAAGGATGGGTCCACCAGTGGGGGGTCACCGTCGGGGCCCAA
  GTCGCTACGGCCCCCAGTATGGGCACCCCCCACCCCCTCCCCCACCACCCGAGTATGGCC
  CTCACGCCGACAGCCCTGTGCTCATGGTCTATGGCTTGGATCAATCTAAGATGAACTGTG
  ACCGAGTCTTCAATGTCTTCTGCTTATATGGCAATGTGGAGAAGGTGAAATTCATGAAAA
  GCAAGCCGGGGGCCGCCATGGTGGAGATGGCTGATGGCTACGCTGTAGACCGGGCCATTA
  CCCACCTCAACAACAACTTCATGTTTGGGCAGAAGCTGAATGTCTGTGTCTCCAAGCAGC
  CAGCCATCATGCCTGGTCAGTCATACGGGTTGGAAGACGGGTCTTGCAGTTACAAAGACT
  TCAGTGAATCCCGGAACAATCGGTTCTCCACCCCAGAGCAGGCAGCCAAGAACCGCATCC
  AGCACCCCAGCAACGTGCTGCACTTCTTCAACGCCCCGCTGGAGGTGACCGAGGAGAACT
  TCTTTGAGATCTGCGATGAGCTGGGAGTGAAGCGGCCATCTTCTGTGAAAGTATTCTCAG
  GCAAAAGTGAGCGCAGCTCCTCTGGACTGCTGGAGTGGGAATCCAAGAGCGATGCCCTGG
  AGACTCTGGGCTTCCTGAACCATTACCAGATGAAAAACCCAAATGGTCCATACCCTTACA
  CTCTGAAGTTGTGTTTCTCCACTGCTCAGCACGCCTCCTAATTAGGTGCCTAGGAAGAGT
  CCCATCTGAGCAGGAAGACATTTCTCTTTCCTTTATGCCATTTTTTGTTTTTGTTATTTG
  CAAAAGATCTTGTATTCCTTTTTTTTTTTTTTTTTTTTTTTTTTAAATGCTAGGTTTGTA
  GAGGCTTACTTAACCTTAATGGAAACGCTGGAAATCTGCAGGGGGAGGGAGAGGGGAACT
  GTTATCTCCCAAGATTAACCTTCACTTTTAAAAAATTATTGGACATGTGATTTTTTTTTT
  TCCTGTTCAAACATTTGTGCTGCCCATGTACTCTTGGCACATTTCAATAAAATTGTTTGG
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001533 unedited
  GACAGAATTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCTGCGCC
  GCCATGTCGCGGAGGCTGCTGCCCCGGGCGGAGAAGCGGCGTCGGCGGCTGGAGCAGAGG
  CAGCAGCCGGACGAGCAGCGGAGGCGGTCGGGAGCGATGGTGAAGATGGCGGCGGCGGGC
  GGCGGAGGCGGCGGTGGCCGCTACTACGGCGGCGGCAGTGAGGGCGGCCGGGCCCCTAAG
  CGGCTCAAGACTGACAACGCCGGCGACCAGCACGGAGGCGGCGGCGGTGGCGGTGGAGGA
  GCCGGGGCGGCGGGCGGCGGCGGCGGTGGGGAGAACTACGATGACCCGCACAAAACCCCT
  GCCTCCCCAGTTGTCCACATCAGGGGCCTGATTGACGGTGTGGTGGAAGCAGACCTTGTG
  GAGGCCTTGCAGGAGTTTGGACCCATCAGCTATGTGGTGGTAATGCCTAAAAAGAGACAA
  GCACTGGTGGAGTTTGAAGATGTGTTGGGGGCTTGCAACGCAGTGAACTACGCAGCCGAC
  AACCAAATATACATTGCTGGTCACCCAGCTTTTGTCAACTACTCTACCAGCCAGAAGATC
  TCCCGCCCTGGGGACTCGGATGACTCCCGGAGCGTGAACAGTGTGCTTCTCTTTACCATC
  CTGAACCCCATTTATTCGATCACCACGGATGTTCTTTACACTATCTGTAATCCTTGTGGG
  CCCTGTCCAGAGAATTGTCATTTTCAGGAAGAATGGAGTTCAGGCGATGGTGGGAATTTG
  ACTCCTTTCAAAGTGCCCCAGCGGGCCCAAGCCTTTCTCAATGGGGCTGATATCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001533 unedited
  CACTTGGAGATGGCAACTTCCAGGNCCAGNANGAGCACTGGGGNGGGGTCACAGGGATGC
  CACCCGGGATCTGTTCNAGAAACAGCTTGACCGCAGCCGCAATCTANAGTCGAGTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAAAC
  AATTTTTTTGAAATGGGCCAAGAGTTCATGGGCCGCCCAATTGTTTGAACCGGAAAAAAA
  AAAATCCCCTGTTCAATTTTTTTTTAAAAGTGAAGGTTAATTTTGGGAGAAAACAGTTNN
  CNTCTCCCTCCCCCTGGAAATTTCCAGGGTTTCCATTAAGGGTAAATAAGCCTTTTCAAA
  CCTNNCTTTTTNAAAAAAAAAAAAAAAAAAAAAAGGGGTTTCCAGTTTTTTTGCCAATAA
  CAAAAACAAAAAATGGCTTTAAGGAAAAAAAAATGTTTTTCTGCTCAAATGGGACTTTTT
  CTAGGCCCCTTATTTAGAGGCGTGCTTAACATTGGAGAAACACAACTTCAAAGTGTAAAG
  GTATGGGACCTTGTGGGTTCCAACTTGGGTATGGTTTCAAGCNGCCTATTTTCTGAGGCA
  TTGTTTTGGAATTCCCACTCCAGCAGTTCAGAGGAGCTGCGCTCACTTTTTGCTGAGAAT
  CTTTCACAGGAGTGGCCGCTTTACTCCCGCTTATGGGAGATTTTAGAGGGTTTTCCTTGG
  TCACCTCCGCGGGGCGTTTAGAAAGATGCAACACTTTTTTGGGGTGCTGGATGGCGTTTT
  TGGCTGCCTGCTTTGGGGGGGAAACCCCATTTTTCCCGGGTTCACTGGATTTTTTTGTCA
  CGGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001533
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001533.2, NP_001524.2
• RefSeq Size: 2129 bp
• RefSeq ORF: 1770 bp
• Locus ID: 3191
• UniProt ID: P14866
• Cytogenetics: 19q13.2
• Domains: RRM
• Gene Summary: Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).