FUT2 (NM_000511) Human Untagged Clone

CAT#: SC313037

FUT2 (untagged)-Human fucosyltransferase 2 (secretor status included) (FUT2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FUT2
• Synonyms: B12QTL1; SE; Se2; SEC2; sej
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000511 edited
  ATGCTGGTCGTTCAGATGCCTTTCTCCTTTCCCATGGCCCACTTCATCCTCTTTGTCTTT
  ACGGTTTCCACTATATTTCACGTTCAGCAGCGGCTAGCGAAGATTCAAGCCATGTGGGAG
  TTACCGGTGCAGATACCAGTGCTAGCCTCAACATCAAAGGCACTGGGACCCAGCCAGCTC
  AGGGGGATGTGGACGATCAATGCAATAGGCCGCCTGGGGAACCAGATGGGCGAGTACGCC
  ACACTGTACGCCCTGGCCAAGATGAACGGGCGGCCCGCCTTCATCCCGGCCCAGATGCAC
  AGCACCCTGGCCCCCATCTTCAGAATCACCCTGCCGGTGCTGCACAGCGCCACGGCCAGC
  AGGATCCCCTGGCAGAACTACCACCTGAATGACTGGATGGAGGAGGAATACCGCCACATC
  CCGGGGGAGTACGTCCGCTTCACCGGCTACCCCTGCTCCTGGACCTTCTACCACCACCTC
  CGCCAGGAGATCCTCCAGGAGTTCACCCTGCACGACCACGTGCGGGAGGAGGCCCAGAAG
  TTCCTGCGGGGCCTGCAGGTGAACGGGAGCCGGCCGGGCACCTTTGTAGGGGTCCATGTT
  CGCCGAGGGGACTATGTCCATGTCATGCCAAAAGTGTGGAAGGGGGTGGTGGCCGACCGG
  CGATACCTACAGCAGGCCCTGGACTGGTTCCGAGCTCGCTACAGCTCCCTCATCTTCGTG
  GTCACCAGTAATGGCATGGCCTGGTGTCGGGAGAACATTGACACCTCCCACGGTGATGTG
  GTGTTTGCTGGCGATGGCATTGAGGGCTCACCTGCCAAAGATTTTGCTCTACTCACACAG
  TGTAACCACACCATCATGACCATTGGGACGTTCGGGATCTGGGCCGCATACCTCACGGGC
  GGAGACACCATCTACCTGGCCAATTACACCCTCCCCGACTCCCCTTTCCTCAAAATCTTT
  AAGCCAGAGGCAGCCTTCCTGCCGGAGTGGACAGGGATTGCCGCAGACCTGTCCCCCTTA
  CTCAAGCACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000511 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGAAGGGTTTCTTTGGCC
  CTGAGTGAAGAGAGACCCAGAGGGAACACTGAGGTGCCTGCCCAACCACTCTGTCCCGGT
  TTCCTTCAGCAGGACCAGGTGAGAGAAGCCATGCTGGTCGTTCAGATGCCTTTCTCCTTT
  CCCATGGCCCACTTCATCCTCTTTGTCTTTACGGTTTCCACTATATTTCACGTTCAGCAG
  CGGCTAGCGAAGATTCAAGCCATGTGGGAGTTACCGGTGCAGATACCAGTGCTAGCCTCA
  ACATCAAAGGCACTGGGACCCAGCCAGCTCAGGGGGATGTGGACGATCAATGCAATAGGC
  CGCCTGGGGAACCAGATGGGCGAGTACGCCACACTGTACGCCCTGGCCAAGATGAACGGG
  CGGCCCGCCTTCATCCCGGCCCAGATGCACAGCACCCTGGCCCCCATCTTCAGAATCACC
  CTGCCGGTGCTGCACAGCGCCACGGCCAGCAGGATCCCCTGGCAGAACTACCACCTGAAT
  GACTGGATGGAGGAGGAATACCGCCACATCCCGGNGGAGTACGTCCGCTTCACCGGCTAC
  CCCTGCTCCTGGACCTTCTACCACCACCTCCGCCAGGAGATCCTCCAGGAGTTCACCCTG
  CACGACCACGTGCGGGAGGAGGCCCANAAGTTCCTGCGGGGCCTGCAGGTGAACGGGAGC
  CGGGCCGGCACCTTTGTAGGGGTCCATGGTCGCCGAGGGGACTATGTCCATGTCATGCCN
  AAAGTGTGGAAGGGGGGTGGGTGGCCGACCGGCGATACCTACAGCAGGCCCTGGACTGGN
  TNCGAGCTCGCTACACTCCTCATCTTCGTGTCACCCCATATGCATGGCCTGGTGTCGGAG
  ACATGACACTCACGTGAATTGNGTGTTGCTGCGATGCATGAGGCTACTGCAAGATTGCCT
  ATCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000511 unedited
  NCACATGTACCCTGCCGCAATCNANGATCGAGTTTTTTTTTTTTTTTTTTGAGGAGGAGT
  CTCACTCTGTTGTCCAGGCTAGAGTGCAATGGTGTGATCCTGGCTCACTGCAACCTCTGC
  CTCCCTGGTGCAAACGATTCTCCTGCTTAAGCCTGCCAAGTAGCTGGGACTACAGGTCTG
  CACCACCACCCCTGGCTAATGTTTTGTATGTTTAGTAGAGACAGGGTTTCACTATGTTGC
  CCAGGCTAGTCTCGAACTCCTGACCTCAGGTGATCCGCCCGCCTTACCCTCCCAAAGTGC
  TGGGATTACAGCCACCATGCCTGGCCTAAGATGTATTTTGGAGTTCATGTGATACTTATG
  TAATACGCTCTCAGAGAACCTTTTCTCCCATCCGCAAAGTCATAATTGTGTTTATTCATT
  TATTTATTTATAAGAGTCAGGGTATCACTACGTCGTCCAGGCCGCAGTGCAGTGGCTATT
  CGCAAGTCCAATCACGGTGCACTATATTCCCTAACTCCTGGCATCAAGCAATCCTCCCAC
  CTCAGCCTCCCTGGTAGCTGGGAATACCCAACTCCATGACTGTATGCACACTGGTGATTA
  AAAAAAATTATTCCTTTGATGACTGTGTAATGGTTACAAATGTATCCTCTGAGAGGGAAG
  GCTGCTGTGGGGGGTTTCACTGCCTGACATGTAACACATGTGCTGCAAACATTTACTGAA
  AGAATGAACGGAACGCCCTGAAGTTCACTGACCCAGGCTGGTCTCTCAGTTCTAGCAATG
  ATCATCCCAGGCCCTAAAAAGCCAGGATTGAAAAGGCGGACCTATGGCCTTGGATGCATG
  CCAGCTACAACCCATATGCCCATTTGTGAAACTGGAAAAAGGTCCCCTTCCAGAGGACTA
  ACTAGAAAAACTGGGCTCCTAGGCCGGGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_000511
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000511.3, NP_000502.3
• RefSeq Size: 3088 bp
• RefSeq ORF: 1032 bp
• Locus ID: 2524
• UniProt ID: Q10981
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - globo series, Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Sequence Note: This RefSeq record represents the SE*01.01.01 allele.