SCF (KITLG) (NM_003994) Human Untagged Clone

CAT#: SC312617

KITLG (untagged)-Human KIT ligand (KITLG), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCF
• Synonyms: DCUA; DFNA69; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; SLF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003994 edited
  GAACAGCTAAACGGAGTCGCCACACCACTGTTTGTGCTGGATCGCAGCGCTGCCTTTCCT
  TATGAAGAAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAA
  TCCTCTCGTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGT
  CACTAAATTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGG
  GATGGATGTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAG
  CTTGACTGATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCAT
  CATAGACAAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATC
  TAAGGATCTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATT
  CTTTAGAATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAAC
  TAGTGATTGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGGGAAGGCCAAAAATCCCCC
  TGGAGACTCCAGCCTACACTGGGCAGCCATGGCATTGCCAGCATTGTTTTCTCTTATAAT
  TGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAGACAGCCAAGTCTTACAAGGGCAGT
  TGAAAATATACAAATTAATGAAGAGGATAATGAGATAAGTATGTTGCAAGAGAAAGAGAG
  AGAGTTTCAAGAAGTGTAATTGTGGCTTGTATCAACACTGTTACTTTCGTACATTGGCTG
  GTAACAGTTCATGTTTGCTTCATAAATGAAGCAGCTTTAAACAAATTCATATTCTGTCTG
  GAGTGACAGACCACATCTTTATCTGTTCTTGCTACCCATGACTTTATATGGATGATTCAG
  AAATTGGAACAGAATGTTTTACTGTGAAACTGGCACTGAATTAATCATCTATAAAGAAGA
  ACTTGCATGGAGCAGGACTCTATTTTAAGGACTGCGGGACTTGGGTCTCATTTAGAACTT
  GCAGCTGATGTTGGAAGAGAAAGCACGTGTCTCAGACTGCATGCACCATTTGCATGGCTC
  CAGAAATGTCTAAATGCTGAAAAAACACCTAGCTTTATTCTTCAGATACAAACTGCAGCC
  TGTAGTTATCCTGGTCTCTGCAAGTAGATTTCAGCTTGGATAGTGAGGGTAACAATTTTT
  CTCAAAGGGATCTAGAAAAAATGTTTAAAACTCAGTAGTGTCAGCCACTGTACAGTGTAG
  AAAGCAGTGGGAACTGTGATTGGATTTGGCAACATGTCAGCTTTATAGTTGCCGATTAGT
  GATATGGGTCTGATTTCGATCTCTTCCTGATGTAAACCATGCTCACCCATATCCCACTAT
  ACAAATGCAAATGGTTGCCTGGTTCCATTTATGCAAGGGAGCCAGTACTGAATTATGCCT
  TGGCAGAGGGGAGACTCCAAAAGAGTCATCGCAGGAAGAAGTTAAGAACACTGAACATCA
  GAACAGTCTGCCAAGAAGGACATTGGCATCCTGGGAAAGTCCGCCTTTTCCCTTGACCAC
  TATAGGGTGTATAAATCGTGTTTGCAAAATGTGTTATGATGTGTTTATATTCTAAAACTA
  TTACAGAGCTATGTAAAGGGACTTAGGAGAAAATGCTGAATGTAAGATGGTCCCATTTCA
  ATTTCCACCATGGGAGAGCCTAAAAATAAATTATGACATTTAGTATCTAAGGTTAGAAAA
  CCACGCCCACATGCTAATATGGGTGTTGAAAACTAGGTTACTTATAATGCAAGGAATCAG
  GAAACTTTAGTTATTTATAGTATAATCACCATTATCTGTTTAAAGGATCCATTTAGTTAA
  AATCGGGCACTCTATATTCATTAAGGTTTATGAATTAAAAAGAAAGCTTTATGTAGTTAT
  GCATGTCAGTTTGCTATTTAAAATGTGTGACAGTGTTTGTCATATTAAGAGTGAATTTGG
  CAGGAATTCCCAAGATGGACATTGTGCTTTTAAACTAGAACTTGTAAGACATTATGTGAA
  TATCCCTTGCCAATTTTTTTTATAATAAGAAAACATCTGACTAAAGTCAAAGAATGATTT
  CTTATGGTTTATTTTGATGAAAGTTCTTTTAACATGTCTTGAATGTACACATAAAGGAAT
  CCAAAGCTTTCCATTCTAACTTAATCTTTGTGATAACATTATTGCCATGTTCTACAACCG
  TAAGATGACAGTTTTCAATGTAGTGACACAAAAGGGCATGAAAAACTAACTGCTAGCTTT
  CCTTTCATTTCAAAAGTCCAAGAATTTCTAGTATATTTGGATTTTAGCTTCTGTTCAAAG
  CAAATCCAGATGCAACTCCAGTAAGTGGCCTTTGCTCTTTTTTGTACCAAAGAGCCCAGA
  TGATTCCTACAGTCCCTTTCTTCTCTAACATGCTGTGGTTCCTTAAATATGAGTAATTTC
  TCTAAGATATAACCCAGGTGCTTTGAGAAGCTGCATTAAGGTGTTCAGGCCCTCAGATAT
  CACATGGTACACTTGATTAGTAATAAAACCAGAGATCAATTTAAATTGCTGATAGGTCCT
  GTCTCAGTGTGTGGCATTGACTGTTTTCAGGAAAATAGATACAGATTAATATGAGTTATG
  CGTGTAGGTTGTGTATAGATTGAGAAGATAGATACTTCTCAATCTAGTAGTTTGATTTAT
  TTAACCAATGGTTTCAGTTTGCTTGAGCATATGAAAATCCTGCTTAATGTGCTTAAGAGT
  ATAATAAATGTGTACTTTTGTCCTCAAACCTAGTAGCTGGGTTTTAACACTCATGGACAT
  GGTCTTAATCAATGGAGTTAAATAAACAAATTCAGCAAGTTATTAAATCTGACATGGTAG
  GAGAGGGGAGATGTGTCCTGCTTATTAAATGTGTTGGTCCATTGAAAGTTACATGGATTG
  CCAATTTTTAAAACACTAAAGTTGAATAAAATGCATGAACAATAGAAAAATGCTGAACAT
  TATTTTGGATGCTAGCTGCTTGGACATTAACTGTGTTATTTCTGCTTTGAGATGAAAATA
  TATATTTATCTTTGCTTATTTTATCCCAGATGTGTTCTGAATATCCTTCTTCATAAATCA
  TGGAAAACTCACTGCTGAGATAGTAAACCATGAAATCGCCTTTTCAGTTGGTGCCATGTA
  TCTGACAGTTCCATCTTGGAAGGTTTCAAAATTACCTTTTAAAATGATCTCAGAAGTCTG
  TAGATTCTCAATGATACTGAAAGCTTTGCACCTCTTTGGTAGAAACCAGGTCTATTTAGA
  AAATGGCTTTATGATAAATGTTGCCTCCTGAGTGATAATGAAGTGTTCCTGGATATTGTA
  TTGTAATTTAATGTGCTTACCACACTGCCACATTTTAATGAGTCAGAGAAAAATTAATTT
  TTCTTCAATACAATAATAGAACAAGTAGCCTATTCTCTTAAAAAGTATGTGAAAAGAAAA
  TTATGAAAAAATATGCATACCTAATGAAGTATTGGTTTTAGTAAGAATTAAATACATTTC
  ATTGAGCTTTAAAGTACTTTGGAGAAACTTTGGGGCACGTTTTCCTACTCTAATTCAACT
  AAAGTTATAAATAAAGAGAAAAACTCATTCAGAAATCATGGATTTTAAAAATATTTTACT
  GCAGCCAAGTTTTCATTTCAAAATGTAATTTCAGTTTGGAGCTTTTAGGCATTATGTATA
  TTTAAAAAATATATTCTTCAAAAATGCATTTTGGCATGGTGGGATGGATGTTGCAAAAGA
  TATCCGGAGCCTCCAGTCTGTCATTAACTGATATGGTAAATCACCTCTCTTCTTTGGGTC
  TCAATTTTTTATTTATCTATATGGTAAACTCAGAGATCACTCCTTAGGGGTGAGTCCTAT
  TGCAATATGACCGACAAAGAAGACAAAATAGCATTGAAACTAACCCATACAAAATATCCA
  ACTCTGGATTCTGTGAATAAGTATCTTGACCATAAAAAGTCATTGCTGTTCTTGTTTCTA
  ATGTAAATAGTGTCCATTAGTAAAAGTGAAATTCAGTCTTAAGTAGGGTGAATTGGATCA
  CCATTTACACAAGAGATGGCTTTTTCCTTTGCTTGAATAAACATTTTGGATCACCTCCAA
  AGAATGAAAACCAGTAGTACGTTTTAGTCATATTAGTCAGGATGAGAAACTATAAGATGT
  GTGTAACATTTGGAAATGCACCAAAGTGAGCGTTTAAATCTTCTCATTTTATTGAAAACT
  AAGAGCAGAAAATGTAAAATGCTCATGAAGGTTTTGAATGCCAAAAGATATTTTAGAATC
  AATTTATAAAGGGGTAATTCATTAATTACACTTTAAAATTGGAAAGTGGGATAAGAAATC
  TAAAGTAAACCAGCTTATCTTTGAAACAATATTATTTTGAAATTGGCTTTAAAATAAAAC
  CATTCAGATTGAAATTCTAATTAGCTCATTTGTGGAGTTTGATCACACAATTCATAATGT
  TGCTGCTTTCCATTAACTAGTCTTGAAATGCCTTTGTTTGTAAAAATAAAATAATGGTAC
  TTTCATTTTATAACAAGGTGTTTTTTTCAAGAAATAATCCATGCTAAAATGGATATTTGT
  GATCCTGAAATGTTTACTAAGCATTGTAAATTTATTTATAACTGCCATCTCCAACTACAT
  CCTTATGATGTTTTTAACAATAAAATTAAAACAACTGTTAAACTAAAAACCACACCGTTT
  TCCAGTACTTGATCTCTGAGCTACAATACTCACTAAATATAATTTTCCAATCAAAATATT
  CTATTCTATATTCTAAGGGTTAATATGTGATTATAGTGTCCACTTGCCACCATTTTTTTA
  AATCAATGGACTTGAAAAGTATTAATTTAGATGGATGCGCAGATATACCCTCAGTTCAGT
  CATAGATTGGAGTTTGCATATAATAATGTAAATGTATGTCGACACTATTCTAAATAGTTC
  TATTATGACTGAAATTTAATTAAATAAAAAAGGTTGTAAAATGTGATGTGTATGTGTATA
  TACTGTATGTGTACTTTTTAAAATAGGTGTATGTCCCAACCCTTTTTTATACAGGTTTGA
  ATTTAAAATTACATGATATATACATATACTTTATTGTTCTAAATAAAGAATTTTATGCAC
  TCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_003994
• Insert Size: 5200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_003994.4.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003994.4, NP_003985.2
• RefSeq Size: 5351 bp
• RefSeq ORF: 738 bp
• Locus ID: 4254
• UniProt ID: P21583
• Cytogenetics: 12q21.32
• Domains: SCF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer
• Gene Summary: This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (a) lacks an alternate in-frame exon, compared to variant b. Isoform a lacks the primary proteolytic-cleavage site; as a result, the protein encoded by isoform a is largely a membrane bound product. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.