ESD (NM_001984) Human Untagged Clone

CAT#: SC310576

ESD (untagged)-Human esterase D (ESD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ESD
• Synonyms: FGH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001984 edited
  CAGCAATTGGACAGGAAAAGAATGGCATTGAAGCAGATTTCCAGCAACAAGTGCTTTGGG
  GGATTGCAGAAAGTTTTTGAACATGACAGTGTTGAACTAAACTGCAAAATGAAATTTGCT
  GTCTACTTACCACCAAAGGCAGAAACAGGAAAGTGCCCTGCACTGTATTGGCTCTCAGGT
  TTAACTTGCACAGAGCAAAATTTTATATCAAAATCTGGTTATCATCAGTCTGCTTCAGAA
  CATGGTCTTGTTGTCATTGCTCCAGATACCAGCCCTCGTGGCTGCAATATTAAAGGTGAA
  GATGAGAGCTGGGACTTTGGCACTGGTGCTGGATTTTATGTTGATGCCACTGAAGATCCT
  TGGAAAACCAACTACAGAATGTACTCTTATGTCACAGAGGAGCTTCCCCAACTCATAAAT
  GCCAATTTTCCAGTGGATCCCCAAAGGATGTCTATTTTTGGCCACTCCATGGGAGGTCAT
  GGAGCTCTGATCTGTGCTTTGAAAAATCCTGGAAAATACAAATCTGTGTCAGCATTTGCT
  CCAATTTGCAACCCTGTACTCTGTCCCTGGGGCAAAAAAGCCTTTAGTGGATATTTGGGA
  ACAGATCAAAGTAAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCCTATCCAGGA
  TCTCAGCTGGACATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAG
  TTACTCCCTGATAACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGTTTTTCGA
  TTGCAAGAGGGTTATGATCATAGCTACTACTTCATTGCAACCTTTATTACTGACCACATC
  AGACATCATGCTAAATACCTGAATGCATGAAAAAACTCCAAATAAGAGAATCTCTTCAGG
  ATTATAAAAGTTGTAAAATGCAACTGTATTGCTGAGCAAAAAAAAAAAAAATTCAAAACA
  TTGGATTTTATAGTGCTAAAAGGGCTTTATTCTATAGTTGAATCACCTCTGAATAAAGAT
  ATAAAACCTAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001984 unedited
  GATATTTGTATACGACTCACTATAGGGCGGCCGCACAATTCGCACGAGGCAGCAATTGGA
  CAGGAAAAGATGGCATTGAAGCAGATTTCCAGCAACAAGTGCTTTGGGGGATTGCAGAAA
  GTTTTTGAACATGACAGTGTTGAACTAAACTGCAAAATGAAATTTGCTGTCTACTTACCA
  CCAAAGGCAGAAACAGGAAAGTGCCCTGCACTGTATTGGCTCTCAGGTTTAACTTGCACA
  GAGCAAAATTTTATATCAAAATCTGGTTATCATCAGTCTGCTTCAGAACATGGTCTTGTT
  GTCATTGCTCCAGATACCAGCCCTCGTGGCTGCAATATTAAAGGTGAAGATGAGAGCTGG
  GACTTTGGCACTGGTGCTGGATTTTATGTTGATGCCACTGAAGATCCTTGGAAAACCAAC
  TACAGAATGTACTCTTATGTCACAGAGGAGCTTCCCCAACTCATAAATGCCAATTTTCCA
  GTGGATCCCCAAAGGATGTCTATTTTTGGCCACTCCATGGGAGGTCATGGAGCTCTGATC
  TGTGCTTTGAAAAATCCTGGAAAATACAAATCTGTGTCAGCATTTGCTCCAATTTGCAAC
  CCTGTACTCTGTCCCTGGGGCAAAAAAGCCTTTAGTGGATATTTGGGAACAGATCAAAGT
  AAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCCTATCCAGGATCTCAGCTGGAC
  ATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAGTTACTCCCTGAT
  AACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGNTTTTCGATTGCAAGAGGTT
  ATGATCATAGCTACTACTTCATTGCCACCTTTATTACTGACCACATCAGACATCATGCTA
  AATACCTCGATGCATGAAAAAACTCCCAAATAGAGAAATCTCTTTCAGATNACTAAAGTT
  GTANAATGCACTGTATTGCTGAGCACAAAAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001984 unedited
  NTTCTAGCTTGNCCGCGGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTAGGTTTT
  ATATCTTTATTCAGAGGTGATTCAACTATAGAATAAAGCCCTTTTAGCACTATAAAATCC
  AATGTTTTGAATTTTTTTTTTTTTTGCTCAGCAATACAGTTGCATTTTACAACTTTTATA
  ATCCTGAAGAGATTCTCTTATTTGGAGTTTTTTCATGCATTCAGGTATTTAGCATGATGT
  CTGATGTGGTCAGTAATAAAGGTTGCAATGAAGTAGTAGCTATGATCATAACCCTCTTGC
  AATCGAAAAACAACGGGGATTTTCTTTTCTGTACAGGCAGCTATGAAGTTATCAGGGAGT
  AACTGTCCATCTAAAAGAAACTGGTCATCTTTCCCTTGATCAATTAGTATGTCCAGCTGA
  GATCCTGGATAGGATTTCACAAGGTGGGTAGCATCATAAGCCTTCCATTTACTTTGATCT
  GTTCCCAAATATCCACTAAAGGCTTTTTTGCCCCAGGGACAGAGTACAGGGTTGCAAATT
  GGAGCAAATGCTGACACAGATTTGTATTTTCCAGGATTTTTCAAAGCACAGATCAGAGCT
  CCATGACCTTCCATGGAGTGGCCAAAAATAGACATCCTTTGGGGATCCACTGGAAAATTG
  GCATTTATGAGTTGGGGAAGCTCCTCTGTGACATAAGAGCACATTCTGTAGTTGGTTCTC
  CAAGATCTTTAGTGGCATCAACATAAAATCAACACCAGTGCCAAACCCCAGCTCTCATCT
  TCACCTTTAATATTGCAGCCCCCCAGGGCTGCGATTTGGAGCAATGACAACAAGACCATG
  TTNTTGAACCACACTGCGATAACCCGATTTTGTACTAAAATTCTGCCCTTGGCCAGTTTA
  AN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001984
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001984.1, NP_001975.1
• RefSeq Size: 1208 bp
• RefSeq ORF: 849 bp
• Locus ID: 2098
• UniProt ID: P10768
• Cytogenetics: 13q14.2
• Gene Summary: This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq, Feb 2009]