LIM1 (LHX1) (NM_005568) Human Untagged Clone

CAT#: SC310433

LHX1 (untagged)-Human LIM homeobox 1 (LHX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIM1
• Synonyms: LIM-1; LIM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005568 edited
  GGGAGCTCGTGCCGATTGTCTTCAGGAGTCATCCCCTGGGCTCTACTTTGCCCCTCTCTC
  TCTCTGGGCCTCATCAGACCAAACCAAAGACCATGGTTCACTGTGCCGGCTGCAAAAGGC
  CCATCCTGGACCGCTTTCTCTTGAACGTGCTGGACAGGGCCTGGCACGTCAAGTGCGTCC
  AGTGCTGTGAATGTAAATGCAACCTGACCGAGAAGTGCTTCTCCAGGGAAGGCAAACTCT
  ACTGCAAGAACGACTTCTTCCGGTGTTTCGGTACCAAATGCGCAGGCTGCGCTCAGGGCA
  TCTCCCCTAGCGACCTGGTGCGGAGAGCGCGGAGCAAAGTGTTTCACCTGAACTGCTTCA
  CCTGCATGATGTGTAACAAGCAGCTCTCCACTGGCGAGGAACTCTACATCATCGACGAGA
  ATAAGTTCGTCTGCAAAGAGGATTACCTAAGTAACAGCAGTGTTGCCAAAGAGAACAGCC
  TTCACTCGGCCACCACGGGCAGTGACCCCAGTTTGTCTCCGGATTCCCAAGACCCGTCGC
  AGGACGACGCCAAGGACTCGGAGAGCGCCAACGTGTCGGACAAGGAAGCGGGTAGCAACG
  AGAATGACGACCAGAACCTGGGCGCCAAGCGGCGGGGACCGCGCACCACCATCAAAGCCA
  AGCAGCTGGAGACGCTGAAGGCCGCCTTCGCTGCTACACCCAAGCCCACCCGCCACATCC
  GCGAGCAGCTGGCGCAGGAGACCGGCCTCAACATGCGCGTCATTCAGGTCTGGTTCCAGA
  ACCGGCGCTCCAAGGAGCGGAGGATGAAGCAGCTGAGCGCCCTGGGCGCCCGGCGCCACG
  CCTTCTTCCGCAGTCCGCGCCGGATGCGGCCGCTGGTGGACCGCCTGGAGCCGGGCGAGC
  TCATCCCCAATGGTCCCTTCTCCTTCTACGGAGATTACCAGAGCGAGTACTACGGGCCCG
  GGGGCAACTACGACTTCTTCCCGCAAGGCCCCCCGTCCTCGCAGGCCCAGACACCAGTGG
  ACCTACCCTTCGTGCCGTCATCTGGGCCGTCCGGGACGCCCCTGGGTGGCCTGGAGCACC
  CGCTGCCGGGCCACCACCCGTCGAGCGAGGCGCAGCGGTTTACCGACATCCTGGCGCACC
  CACCCGGGGACTCGCCCAGCCCCGAGCCCAGCCTGCCCGGGCCTCTGCACTCCATGTCGG
  CCGAGGTCTTCGGACCCAGCCCGCCCTTCTCGTCGCTGTCGGTCAACGGTGGGGCGAGCT
  ACGGAAACCACCTGTCCCACCCCCCCGAAATGAACGAGGCGGCCGTGTGGTAGCGGGGTC
  TCGCACGGTCTGCGGAGTTCGT
  
• Restriction Sites: Please inquire
• ACCN: NM_005568
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_005568.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005568.2, NP_005559.2
• RefSeq Size: 2283 bp
• RefSeq ORF: 1221 bp
• Locus ID: 3975
• UniProt ID: P48742
• Cytogenetics: 17q12
• Domains: homeobox, LIM
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq, Dec 2010]