PANK2 (NM_153638) Human Untagged Clone

CAT#: SC310376

PANK2 (untagged)-Human pantothenate kinase 2 (PANK2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PANK2
• Synonyms: C20orf48; HARP; HSS; NBIA1; PKAN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_153638 edited
  GGGTGGATGAGGAGGCTCGGGCCCTTCCACCCACGCGTCCATTGGGCGGCGCCGCCATCA
  CTCTCTTCTGGGCTACACCGCCTTCTCTTCCTCCGCGGAACCCGGATCCCCTCCTCCACC
  ACCCTCTCCCCGCCCCGTCACGATAGCCTCTCATTGGACGGAGGCACGGTCAATCCTCCT
  CGAGTTAGGGAGCCGACTGGACGCGAGGCCTTTGGGCCGTCCCCAGCCTCGTCGGATTGG
  CTTCCTGCGCGTTGGCGCAACGGAAGAGGCGGCCGGCCGAGGGCGCGCCTCTGCTCTGGC
  TGGACTGCCGCGGAGGAGGCGAGAAGGAATCCGACGCTGGGGGGCTTGCTCGGGCGGCAG
  CGACTGCTGCTGCGGATGGGAGGGGGCCGGCTCGGCGCGCCCATGGAGCGCCACGGCAGG
  GCTTCCGCCACCTCCGTCTCGTCGGCTGGGGAGCAGGCGGCCGGGGACCCCGAAGGGCGG
  CGGCAGGAGCCACTGCGGCGCCGGGCGAGCAGCGCGTCGGTGCCCGCGGTCGGGGCCTCG
  GCTGAGGGCACGAGGCGGGATCGACTGGGCTCTTACAGCGGCCCCACCTCGGTCTCCCGC
  CAGCGCGTCGAAAGCCTGAGGAAAAAGCGGCCGCTTTTTCCATGGTTTGGACTGGATATC
  GGTGGAACTCTGGTCAAGCTGGTATATTTTGAACCCAAAGACATCACTGCTGAAGAAGAA
  GAGGAAGAAGTGGAAAGTCTTAAAAGCATTCGGAAGTACCTGACCTCCAATGTGGCTTAT
  GGGTCTACAGGCATTCGGGACGTGCACCTCGAGCTGAAGGACCTGACTCTGTGTGGACGC
  AAAGGCAATCTGCACTTTATACGCTTTCCCACTCATGACATGCCTGCTTTTATTCAAATG
  GGCAGAGATAAAAACTTCTCGAGTCTCCACACTGTCTTTTGTGCCACTGGAGGTGGAGCG
  TACAAATTTGAGCAGGATTTTCTCACAATAGGTGATCTTCAGCTTTGCAAACTGGATGAA
  CTAGATTGCTTGATCAAAGGAATTTTATACATTGACTCAGTCGGATTCAATGGACGGTCA
  CAGTGCTATTACTTTGAAAACCCTGCTGATTCTGAAAAGTGTCAGAAGTTACCATTTGAT
  TTGAAAAATCCGTATCCTCTGCTTCTGGTGAACATTGGCTCAGGGGTTAGCATCTTAGCA
  GTATATTCCAAAGATAATTACAAACGGGTCACAGGTACTAGTCTTGGAGGAGGAACTTTT
  TTTGGTCTCTGCTGTCTTCTTACTGGCTGTACCACTTTTGAAGAAGCTCTTGAAATGGCA
  TCTCGTGGAGATAGCACCAAAGTGGATAAACTAGTACGAGATATTTATGGAGGGGACTAT
  GAGAGGTTTGGACTGCCAGGCTGGGCTGTGGCTTCAAGCTTTGGAAACATGATGAGCAAG
  GAGAAGCGAGAGGCTGTCAGTAAAGAGGACCTGGCCAGAGCGACTTTGATCACCATCACC
  AACAACATTGGCTCAATAGCAAGAATGTGTGCCCTTAATGAAAACATTAACCAGGTGGTA
  TTTGTTGGAAATTTCTTGAGAATTAATACGATCGCCATGCGGCTTTTGGCATATGCTTTG
  GATTATTGGTCCAAGGGGCAGTTGAAAGCACTTTTTTCGGAACACGAGGGTTATTTTGGA
  GCTGTTGGAGCACTCCTTGAGCTGTTGAAGATCCCGTGATCATTACCTGGGGAGGGGTTC
  CTGAAACCTTCCACAATGGGATCTGTGGACTTTCATTTTTTTAAGAGACTTACTCAATTT
  CATGACTGTACTACCTGAAACAAAGTGAGAAAGGACAGGTGTATTTTTCTAAGTCATCAA
  GATAAATCCTTAAGAATTCAGTCTAAATTAGCAACCAGGAAGGAAAAATATATTAAAAAC
  AACAAAAAAGTGGCACATGTCCAGGCAGTGTGAGGATTTGCTGTATATAAGTTGCCTGCT
  TTGTATTTTTGAAATCTCTGCATCACTCATTGGAAGTGCTTCTGAAGAGAGCTGCTCTGT
  GTTCAGTTGACTGGTTTTGTGTCCTGTTTGAACTTGCTGAATGTAAGGCAGGCTACTATG
  CGTTATAATCTAATCACAATTTGTCAATATGGTCTTGGCAATCATCTGTGCATTACTCTG
  GTTTGCATTAAGCCTGTGTGTGAACTTACTGTAAAACATGTTTT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153638 unedited
  GGTTTCATTTGTATACCACTCATATAGGGCGGCCGCGATTCATGAGAGGCTCGGGCCCTT
  CCACCCACGCGTCCATTGGGCGGCGCCGCCATCACTCTCTTCTGGGCTACACCGCCTTCT
  CTTCCTCCGCGGAACCCGGATCCCCTCCTCCACCACCCTCTCCCCGCCCCGTCACGATAG
  CCTCTCATTGGACGGAGGCACGGTCAATCCTCCTCGAGTTAGGGAGCCGACTGGACGCGA
  GGCCTTTGGGCCGTCCCCAGCCTCGTCGGATTGGCTTCCTGCGCGTTGGCGCAACGGAAG
  AGGCGGCCGGCCGAGGGCGCGCCTCTGCTCTGGCTGGACTGCCGCGGAGGAGGCGAGAAG
  GAATCCGACGCTGGGGGGCTTGCTCGGGCGGCAGCGACTGCTGCTGCGGATGGGAGGGGG
  CCGGCTCGGCGCGCCCATGGAGCGCCACGGCAGGGCTTCCGCCACCTCCGTCTCGTCGGC
  TGGGGAGCAGGCGGCCGGGGACCCCGAAGGGCGGCGGCAGGAGCCACTGCGGCGCCGGGC
  GAGCAGCGCGTCGGTGCCCGCGGTCGGGGCCTCGGCTTGAGGCACGAGGCGGGATCGACT
  GGGCTCTTACAGCGGCCCCCACCTCGGTCTCCCGCCCGCGCGTCCAAAGCCTGAAGGAAA
  AGCGGCCCGCTTTTTCCAGGGTTTGGCCTGGATATCCGTGGAACCTCTGGCAAAGCTGGT
  ATATTTTGGACCCCAAAGACTTCCCTGCTTGAAAAAAACAAGGAAGAAATGGAAAGTCTT
  AAAAGCCTTTCGGAATTCCTTGCCCCCCAATGTGGGCTAATTGGGCCTACAGGCATTCGG
  GGACACGCCACCCCTGAGCTGAAAGGACCTGACTCTTGGTGGGCCCCAAAGCGCATCT
  
• Restriction Sites: Please inquire
• ACCN: NM_153638
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153638.2, NP_705902.2
• RefSeq Size: 2280 bp
• RefSeq ORF: 1713 bp
• Locus ID: 80025
• UniProt ID: Q9BZ23
• Cytogenetics: 20p13
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis
• Gene Summary: This gene encodes a protein belonging to the pantothenate kinase family and is the only member of that family to be expressed in mitochondria. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by acyl CoA species. Mutations in this gene are associated with HARP syndrome and pantothenate kinase-associated neurodegeneration (PKAN), formerly Hallervorden-Spatz syndrome. Alternative splicing, involving the use of alternate first exons, results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest and mitochondrially localized isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.