AKT3 (NM_181690) Human Untagged Clone

CAT#: SC310362

AKT3 (untagged)-Human v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKT3
• Synonyms: MPPH; MPPH2; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_181690 edited
  CCAAACCCTAAAGCTGATATCACAAAGTACCATTTCTCCAAGTTGGGGGCTCAGAGGGGA
  GTCATCATGAGCGATGTTACCATTGTGAAAGAAGGTTGGGTTCAGAAGAGGGGAGAATAT
  ATAAAAAACTGGAGGCCAAGATACTTCCTTTTGAAGACAGATGGCTCATTCATAGGATAT
  AAAGAGAAACCTCAAGATGTGGATTTACCTTATCCCCTCAACAACTTTTCAGTGGCAAAA
  TGCCAGTTAATGAAAACAGAACGACCAAAGCCAAACACATTTATAATCAGATGTCTCCAG
  TGGACTACTGTTATAGAGAGAACATTTCATGTAGATACTCCAGAGGAAAGGGAAGAATGG
  ACAGAAGCTATCCAGGCTGTAGCAGACAGACTGCAGAGGCAAGAAGAGGAGAGAATGAAT
  TGTAGTCCAACTTCACAAATTGATAATATAGGAGAGGAAGAGATGGATGCCTCTACAACC
  CATCATAAAAGAAAGACAATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACT
  TTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATT
  CTGAAGAAAGAAGTCATTATTGCAAAGGATGAAGTGGCACACACTCTAACTGAAAGCAGA
  GTATTAAAGAACACTAGACATCCCTTTTTAACATCCTTGAAATATTCCTTCCAGACAAAA
  GACCGTTTGTGTTTTGTGATGGAATATGTTAATGGGGGCGAGCTGTTTTTCCATTTGTCG
  AGAGAGCGGGTGTTCTCTGAGGACCGCACACGTTTCTATGGTGCAGAAATTGTCTCTGCC
  TTGGACTATCTACATTCCGGAAAGATTGTGTACCGTGATCTCAAGTTGGAGAATCTAATG
  CTGGACAAAGATGGCCACATAAAAATTACAGATTTTGGACTTTGCAAAGAAGGGATCACA
  GATGCAGCCACCATGAAGACATTCTGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTA
  GAAGATAATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATGTATGAA
  ATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACCATGAGAAACTTTTTGAATTAATA
  TTAATGGAAGACATTAAATTTCCTCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCA
  GGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCAAAAGAA
  ATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGCAAGATGTATATGATAAAAAGCTT
  GTACCTCCTTTTAAACCTCAAGTAACATCTGAGACAGATACTAGATATTTTGATGAAGAA
  TTTACAGCTCAGACTATTACAATAACACCACCTGAAAAATGTCAGCAATCAGATTGTGGC
  ATGCTGGGTAACTGGAAAAAATAAT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181690 unedited
  NATTTGCATTTGTAAACGACTCATATAGGCGGCCGCGCAATTCGCACGAGGCCAAACCCT
  AAAGCTGATATCACAAAGTACCATTTCTCCAATTGGGGGCTCAGAGGGGAGTCATCATGA
  GCGATGTTACCATTGTGAAAGAAGGTTGGGTTCAGAAGAGGGGAGAATATATAAAAAACT
  GGAGGCCAAGATACTTCCTTTTGAAGACAGATGGCTCATTCATAGGATATAAAGAGAAAC
  CTCAAGATGTGGATTTACCTTATCCCCTCAACAACTTTTCAGTGGCAAAATGCCAGTTAA
  TGAAAACAGAACGACCAAAGCCAAACACATTTATAATCAGATGTCTCCAGTGGACTACTG
  TTATAGAGAGAACATTTCATGTAGATACTCCAGAGGAAAGGGAAGAATGGACAGAAGCTA
  TCCAGGCTGTAGCAGACAGACTGCAGAGGCAAGAAGAGGAGAGAATGAATTGTAGTCCAA
  CTTCACAAATTGATAATATAGGAGAGGAAGAGATGGATGCCTCTACAACCCATCATAAAA
  GAAAGACAATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAG
  TTATTTTGGTTCGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATTCTGAAGAAAG
  AAGTCATTATTGCAAAGGATGAAGTGGCACACACTCTAACTGANAGCAGAGTATTAAAGA
  ACACTAGACATCCCTTTTTAACATCCTTGAAATATTCCTTCCAGACAAAAGACCGTTTGT
  GTTTTGTGATGGAATATGTTAATGNGGGGCGAGCTGTTTTTCCATTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181690 unedited
  CATGGGGAGGCACTTCAGGCCAGGAAGCACTGGGGAGGGTCACAGGAGCCCCCGGGATCT
  GTTCAGAAACAGCTATGACCGCGGCCGCAATCTAGATTATTTTTTCCAGTTACCCAGCAT
  GCCACAATCTGATTGCTGACATTTTTCAGGTGGTGTTATTGTAATAGTCTGAGCTGTAAA
  TTCTTCATCAAAATATCTAGTATCTGTCTCAGATGTTACTTGAGGTTTAAAAGGAGGTAC
  AAGCTTTTTATCATATACATCTTGCCAGTTTACTCCAGAGAAGAAACTGTGTCTCATAAT
  TTCTTTTGCATCATCTGGTCCTCCACCAAGGCGTTTATTTGGATCCTTTATCAAGAGCCC
  TGAAAGCAATGATTTTGCATCTGAAGAGAGTGTTCGAGGAAATTTAATGTCTTCCATTAA
  TATTAATTCAAAAAGTTTCTCATGGTCCTGGTTGTAGAAAGGTAACCTCCCACACATCAT
  TTCATACATGACAACCCCTAGGCCCCACCAGTCTACTGCTCGGCCATAGTCATTATCTTC
  TAACACCTCTGGTGCCAGATATTCTGGAGTGCCACAGAATGTCTTCATGGTGGCTGCATC
  TGTGATCCCTTCTTTGCAAAGTCCAAATCTGNTATTTTTATGTGGCCATCTTTGTCCAGC
  ATTAGATTCTCCAACTTGAGATCACGGTACACAATCTTTCCGGAATGTAGATAGTCCCAA
  GGCAGAAGACATTTCTGCACCATAGAAACGTGGTGCGGTCCTCAGAAGAACACCCGCTCT
  CTCGACAAATGGAAAAACAGCTCGCCCCCCTTAACATATTTCATCACAAACACAAACGGT
  CTTTTGTCCTGGGAAGGATATTTCAAGGATGTTAAAAAAGGAGGCCAAATGGTCCTTTAT
  AACTCTGCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_181690
• Insert Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181690.1, NP_859029.1
• RefSeq Size: 1703 bp
• RefSeq ORF: 1398 bp
• Locus ID: 10000
• UniProt ID: Q9Y243
• Cytogenetics: 1q43-q44
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region, compared to variant 1. The resulting isoform (2) is shorter and has has a distinct C-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.