BTD (NM_000060) Human Untagged Clone

SKU SC310282

BTD (untagged)-Human biotinidase (BTD)

Product Data

• Type: Human Untagged Clone
• Target Symbol: BTD
• Synonyms: biotinidase
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000060 edited; ATGGCGCATGCGCATATTCAGGGCGGAAGGCGCGCTAAGAGCAGATTTGTGGTCTGCATT; ATGTCTGGAGCCAGAAGTAAGCTTGCTCTTTTCCTCTGCGGCTGTTACGTGGTTGCCCTG; GGAGCCCACACCGGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGTGGCT; GCCGTGTATGAGCATCCATCCATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCGCCAA; GAGGCCTTGGAGCTCATGAACCAGAACCTTGACATCTATGAACAGCAAGTGATGACTGCA; GCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGATGGCATTCATGGATTCAACTTT; ACAAGAACATCCATTTATCCATTTTTGGACTTCATGCCGTCTCCCCAGGTGGTCAGGTGG; AACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGAGTTGT; ATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTTGTCAT; AGCAGTGACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCGTGTTC; AGCAATAATGGAACCCTTGTTGACCGCTACCGTAAACACAACCTCTACTTTGAGGCAGCA; TTCGATGTTCCTCTTAAAGTGGATCTCATCACCTTTGATACCCCCTTTGCTGGCAGGTTT; GGCATCTTCACATGCTTTGATATATTGTTCTTTGACCCTGCCATCAGAGTCCTCAGAGAC; TACAAGGTGAAGCATGTTGTGTACCCAACTGCCTGGATGAACCAGCTCCCACTCTTGGCA; GCAATTGAGATTCAGAAAGCTTTTGCTGTTGCCTTTGGCATCAACGTTCTGGCAGCTAAT; GTCCACCACCCAGTTCTGGGGATGACAGGAAGTGGCATACACACCCCTCTGGAGTCCTTT; TGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAATTGCCCAGGTGGCCAAAAAT; CCAGTGGGTCTCATTGGTGCAGAGAATGCAACAGGTGAAACGGACCCATCCCATAGTAAG; TTTTTAAAAATTTTGTCAGGTGATCCGTACTGTGAGAAGGATGCTCAGGAAGTCCACTGT; GATGAGGCCACCAAGTGGAACGTGAATGCTCCTCCCACATTTCACTCTGAGATGATGTAT; GACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAAGGCTATCTCCACGTCTGTTCCAAT; GGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATCCAAAGAGCTGTATGCCCTG; GGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTACATCCAAGTGTGTGCCCTG; GTCAGGTGTGGGGGTCTTGGCTTCGACACCTGCGGACAGGAAATCACAGAGGCCACGGGG; ATATTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTATATCTTTCCTTTGTTTCTG; ACCTCAGGGATGACCCTAGAAGTCCCTGACCAGCTTGGCTGGGAGAATGACCACTATTTC; CTGAGGAAAAGTAGGCTGTCCTCTGGGCTGGTGACGGCGGCTCTCTATGGGCGCTTGTAT; GAGAGGGACTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000060 unedited; CCCACAGGGACACAACTATACAGGGCGGCCGCGACCCGCACGACAAACAGGTAGAGCCGA; CCCCGAGCCTCCGCCCCGCCCCCGAGACGGCCAGCCGGAGCGAATTCGGGGCTGTAAAGG; GAGAATGGCGCATGCGCAAATTCAGGGCGGAAGGCGCGCTAAGAGCAGACCCGTGGCCCG; CAACATGCCTGGAGCCAGAAGCAAGCCCGCTCCCCTCCTCCGCGGCTGTCACGCGGTCGC; CCTGGGAGCCCACACCGGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGT; GGCTGCCGTGTATGAGCATCCATCCATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCG; CCAAGAGGCCTTGGAGCTCATGAACCAGAACCTTGACATCTATGAACAGCAAGCGATGAC; TGCAGCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGATGGCATTCATGGATTCAA; CCTTACAAGAACATCCATCTTATCCATTTTTGGACTTCATGCCGTCTCCCCAGGTGGTCA; GGTGGAACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGA; GTTGTATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTT; GTCATAGCAGTGACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCG; TGTTCAGCAATAATGGAACCCTTGTTGACCGCTACCGTAACACAACCTCTACTTTGAGCA; GCATTCGATGTTCCTCTTAAAGTGGATCTCATCACCTTTGATACCCCCTTTGCTGGCAGT; TTGGCATCTTCACATGCTTTGATAATATTGTTCT;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000060 unedited; CATCATACGTTCTGCGCTATGTCCCCACCCAGTTCTGGGGATGACAGGAAGTGGCATACA; CACCCCTCTGGAGTCCTTTTGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAAT; TGCCCAGGTGGCCAAAAATCCAGTGGGTCTCATTGGTGCAGAGAATGCAACAGGTGAAAC; GGACCCATCCCATAGTAAGTTTTTAAAAATTTTGTCAGGTGATCCGTACTGTGAGAAGGA; TGCTCAGGAAGTCCACTGTGATGAGGCCACCAAGTGGAACGTGAATGCTCCTCCCACATT; TCACTCTGAGATGATGTATGACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAAGGCTA; TCTCCACGTCTGTTCCAATGGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATC; CAAAGAGCTGTATGCCCTGGGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTA; CATCCAAGTGTGTGCCCTGGTCAGGTGTGGGGGTCTTGGCTTCGACACCTGCGGACAGGA; AATCACAGAGGCCACGGGGATATTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTA; TATCTTTCCTTTGTTTCTGACCTCAGGGATGACCCTAGAAGTCCCTGACCAGCTTGGCTG; GGAGAATGACCACTATTTTCCTGAGGAAAAGTAGGCTGTCCTCTGGGCTGGTGACGGCGG; CTCTCTATGGGCGCTTGTATGAGAGGGACTAGGAAAAGTGTGTGGTCTGTGGGGCGGACT; CTGGCCATCATGTTGACAGCCTTGCACTTCCACAGGCTACAAGCCCTGGGACCATCTTTC; TGCCTTAAGGCAGGAGCCCCAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000060
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000060.2, NP_000051.1
• RefSeq Size: 2082 bp
• RefSeq ORF: 1632 bp
• Locus ID: 686
• UniProt ID: P43251
• Cytogenetics: 3p25.1
• Domains: CN_hydrolase
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Biotin metabolism, Metabolic pathways
• Summary: The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]; Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1.