POC5 (NM_152408) Human Untagged Clone

CAT#: SC310274

POC5 (untagged)-Human POC5 centriolar protein homolog (Chlamydomonas) (POC5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POC5
• Synonyms: C5orf37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310274 representing NM_152408.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGTGGGAAGAATATGAAGAACTGCTTCATTATGCTATAGTGACTCCAAATATTGAACCCTGTGCT
  TCACAGTCATCTCATCCTAAGGGAGAATTGGTGCCAGATGTCAGAATTTCTACAATTCATGATATTCTT
  CATAGTCAAGGAAATAACTCTGAAGTAAGAGAAACTGCAATAGAAGTTGGAAAAGGATGTGATTTCCAT
  ATTTCAAGTCATTCAAAGACAGATGAGTCATCACCAGTGTTATCGCCAAGGAAGCCTTCTCACCCAGTC
  ATGGATTTTTTCAGTTCACATCTTTTAGCTGACTCTTCCTCACCAGCAACAAATTCTAGTCATACAGAT
  GCCCATGAAATACTTGTGAGCGATTTTCTTGTTTCTGATGAAAACCTTCAGAAGATGGAAAATGTGCTT
  GATCTTTGGAGTTCAGGTCTTAAGACAAACATCATATCTGAACTAAGTAAATGGAGACTTAATTTTATT
  GACTGGCACCGAATGGAAATGAGAAAAGAGAAAGAAAAACATGCAGCACATTTAAAACAACTGTGTAAT
  CAGATCAATGAATTGAAGGAGCTGCAAAAAACCTTTGAAATCTCCATTGGGAGAAAAGATGAGGTGATT
  TCTAGCTTGTCTCATGCCATAGGCAAGCAAAAGGAAAAGATAGAGTTGATGAGAACATTCTTCCACTGG
  CGAATCGGCCATGTCAGAGCCAGACAGGATGTTTATGAAGGTAAACTAGCTGACCAGTACTACCAGAGA
  ACTTTACTGAAGAAAGTCTGGAAAGTCTGGCGTTCCGTAGTGCAAAAGCAGTGGAAAGATGTGGTAGAA
  AGAGCTTGTCAAGCAAGAGCTGAAGAAGTTTGTATCCAGATTTCCAATGATTATGAAGCCAAAGTTGCT
  ATGTTATCTGGAGCTTTGGAAAATGCAAAAGCTGAGATTCAAAGAATGCAACATGAAAAAGAGCACTTT
  GAAGATTCCATGAAAAAAGCTTTCATGAGGGGTGTATGTGCATTAAATCTTGAAGCCATGACTATATTT
  CAAAACAGAAATGATGCAGGGATAGACTCCACAAATAATAAAAAGGAAGAGTATGGTCCTGGTGTTCAA
  GGAAAAGAACATTCTGCTCATTTGGATCCTTCAGCTCCTCCGATGCCCTTACCAGTTACATCACCACTG
  CTGCCATCCCCACCAGCCGCCGTCGGAGGAGCCAGCGCGACTGCCGTTCCCTCAGCTGCTTCGATGACT
  TCTACCAGGGCTGCTTCCGCATCTTCTGTTCACGTTCCTGTTTCTGCTCTTGGTGCAGGATCTGCAGCT
  ACTGCTGCATCAGAAGAAATGTATGTGCCAAGAGTTGTAACCTCTGCACAACAGAAAGCAGGAAGAACT
  ATTACAGCCCGGATCACAGGAAGATGTGATTTTGCTTCAAAAAATAGAATTAGCAGCAGTTTAGCTATA
  ATGGGAGTTTCTCCTCCCATGAGCTCAGTTGTTGTGGAAAAACATCATCCAGTCACAGTGCAAACCATT
  CCTCAAGCAACTGCAGCAAAATATCCCCGGACCATTCATCCTGAAAGTAGTACCTCAGCTTCCAGATCA
  CTTGGAACCAGATCAGCTCACACCCAGTCTCTCACAAGTGTTCATTCCATAAAAGTGGTTGACTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_152408
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152408.2
• RefSeq Size: 1969 bp
• RefSeq ORF: 1653 bp
• Locus ID: 134359
• UniProt ID: Q8NA72
• Cytogenetics: 5q13.3
• MW: 60.8 kDa
• Gene Summary: Essential for the assembly of the distal half of centrioles, required for centriole elongation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a unique start codon, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1.