ADAM22 (NM_016351) Human Untagged Clone

CAT#: SC310071

ADAM22 (untagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAM22
• Synonyms: ADAM 22; DEE61; EIEE61; MDC2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016351 edited
  ATGCAGGCGGCAGTGGCTGTGTCCGTGCCCTTCTTGCTGCTCTGTGTCCTGGGGACCTGC
  CCTCCGGCGCGCTGCGGCCAGGCAGGAGACGCCTCATTGATGGAGCTAGAGAAGAGGAAG
  GAAAACCGCTTCGTGGAGCGCCAGAGCATCGTGCCACTGCGCCTCATCTACCGCTCGGGC
  GGCGAAGACGAAAGTCGGCACGACGCGCTCGACACGCGGGTGCGGGGCGACCTCGGTGGC
  CGGCAGTTGACTCATGTTGACCAAGCAAGCTTCCAGGTTGATGCCTTTGGAACGTCATTC
  ATTCTCGATGTCGTGCTAAATCATGATTTGCTGTCCTCTGAATACATAGAGAGACACATT
  GAACATGGAGGCAAGACTGTGGAAGTTAAAGGAGGAGAGCACTGTTACTACCAGGGCCAT
  ATCCGAGGAAACCCTGACTCATTTGTTGCATTGTCAACATGCCACGGACTTCATGGGATG
  TTCTATGACGGGAACCACACATATCTCATTGAGCCAGAAGAAAATGACACTACTCAAGAG
  GATTTCCATTTTCATTCAGTTTACAAATCCAGACTGTTTGAATTTTCCTTGGATGATCTT
  CCATCTGAATTTCAGCAAGTAAACATTACTCCATCAAAATTTATTTTGAAGCCAAGACCA
  AAAAGGAGTAAACGGCAGCTTCGTCGATATCCTCGTAATGTAGAAGAAGAAACCAAATAC
  ATTGAACTGATGATTGTGAATGATCACCTTATGTTTAAAAAACATCGGCTTTCCGTTGTA
  CATACCAATACCTATGCGAAATCTGTGGTGAACATGGCAGATTTAATATATAAAGACCAA
  CTTAAGACCAGGATAGTATTGGTTGCTATGGAAACCTGGGCGACTGACAACAAGTTTGCC
  ATATCTGAAAATCCATTGATCACCCTACGTGAGTTTATGAAATACAGGAGGGATTTTATC
  AAAGAGAAAAGTGATGCAGTTCACCTTTTTTCGGGAAGTCAATTTGAGAGTAGCCGGAGC
  GGGGCAGCTTATATTGGTGGGATTTGCTCGTTGCTGAAAGGAGGAGGCGTGAATGAATTT
  GGGAAAACTGATTTAATGGCTGTTACACTTGCCCAGTCATTAGCCCATAATATTGGTATT
  ATCTCAGACAAAAGAAAGTTAGCAAGTGGTGAATGTAAATGCGAGGACACGTGGTCCGGG
  TGCATAATGGGAGACACTGGCTATTATCTTCCTAAAAAGTTCACCCAGTGTAATATTGAA
  GAGTATCATGACTTCCTGAATAGTGGAGGTGGTGCCTGCCTTTTCAACAAACCTTCTAAG
  CTTCTTGATCCTCCTGAGTGTGGCAATGGCTTCATTGAAACTGGAGAGGAGTGTGATTGT
  GGAACCCCGGCCGAATGTGTCCTTGAAGGAGCAGAGTGTTGTAAGAAATGCACCTTGACT
  CAAGACTCTCAATGCAGTGACGGTCTTTGCTGTAAAAAGTGCAAGTTTCAGCCTATGGGC
  ACTGTGTGCCGAGAAGCAGTAAATGATTGTGATATTCGTGAAACGTGCTCAGGAAATTCA
  AGCCAGTGTGCCCCTAATATTCATAAAATGGATGGATATTCATGTGATGGTGTTCAGGGA
  ATTTGCTTTGGAGGAAGATGCAAAACCAGAGATAGACAATGCAAATACATTTGGGGGCAA
  AAGGTGACAGCATCAGACAAATATTGCTATGAGAAACTGAATATTGAAGGGACGGAGAAG
  GGTAACTGTGGGAAAGACAAAGACACATGGATACAGTGCAACAAACGGGATGTGCTTTGT
  GGTTACCTTTTGTGTACCAATATTGGCAATATCCCAAGGCTTGGAGAACTCGATGGTGAA
  ATCACATCTACTTTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGTGGGCATGTT
  AAGCTTGAAGAAGATGTAGATCTTGGCTATGTGGAAGATGGGACACCTTGTGGTCCCCAA
  ATGATGTGCTTAGAACACAGGTGTCTTCCTGTGGCTTCTTTCAACTTTAGTACTTGCTTG
  AGCAGTAAAGAAGGCACTATTTGCTCAGGAAATGGAGTTTGCAGTAATGAGCTGAAGTGT
  GTGTGTAACAGACACTGGATAGGTTCTGATTGCAACACTTACTTCCCTCACAATGATGAT
  GCAAAGACTGGTATCACTCTGTCTGGCAATGGTGTTGCTGGCACCAATATCATAATAGGC
  ATAATTGCTGGCACCATTTTAGTGCTGGCCCTCATATTAGGAATAACTGCGTGGGGTTAT
  AAAAACTATCGAGAACAGAGGTCAAATGGGCTCTCTCATTCTTGGAGTGAAAGGATTCCA
  GACACAAAACATATTTCAGACATCTGTGAAAATGGGCGACCTCGAAGTAACTCTTGGCAA
  GGTAACCTGGGAGGCAACAAAAAGAAAATCAGAGGCAAAAGATTTAGACCTCGGTCTAAT
  TCAACTGAGACTTTATCTCCTGCCAAGTCTCCTTCTTCATCAACTGGGTCTATTGCCTCC
  AGCAGAAAATACCCTTACCCAATGCCTCCACTTCCTGATGAGGACAAGAAAGTGAACCGA
  CAAAGTGCCAGGCTATGGGAGACATCCATTTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_016351
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016351.3, NP_057435.2
• RefSeq Size: 3347 bp
• RefSeq ORF: 2613 bp
• Locus ID: 53616
• Cytogenetics: 7q21.12
• Domains: Reprolysin, DISIN, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (3), also known as alpha, lacks an in-frame exon compared to variant 1. The encoded isoform (3) is shorter than isoform 1. The encoded isoform (3) may undergo proteolytic processing. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.