ILF3 (NM_012218) Human Untagged Clone

CAT#: SC310057

ILF3 (untagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ILF3
• Synonyms: CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF90; NF90a; NF90b; NF90c; NF90ctv; NF110; NF110b; NFAR; NFAR-1; NFAR-2; NFAR2; NFAR90; NFAR110; TCP80; TCP110
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012218 edited
  CCGGCGCGCGCCTGCCCGCCCGCCCGCTCGCCCCCGGTCCGGACTCCTCCTCCTCCTCTT
  CTCGCCATTGCAGTTGGACCCAGCAGCCCGGCGCGCACCGCGTGGCTTTTGGGGGCAGAC
  CCCGGCGGGCTGTGGCAGGAGGGCGGCGGCGGCGGCTGCGGTCGAAGAAGGGGACGCCGA
  CAAGAGTTGAAGTATTGATAACACCAAGGAACTCTATCACAATTTGAAAAGATAAGCAAA
  AGTTTGATTTCCAGACACTACAGAAGAAGTAAAAATGCGTCCAATGCGAATTTTTGTGAA
  TGATGACCGCCATGTGATGGCAAAGCATTCTTCCGTTTATCCAACACAAGAGGAGCTGGA
  GGCAGTCCAGAACATGGTGTCCCACACGGAGCGGGCGCTCAAAGCTGTGTCCGACTGGAT
  AGACGAGCAGGAAAAGGGTAGCAGCGAGCAGGCAGAGTCCGATAACATGGATGTGCCCCC
  AGAGGACGACAGTAAAGAAGGGGCTGGGGAACAGAAGACGGAGCACATGACCAGAACCCT
  GCGGGGAGTGATGCGGGTGGGCCTGGTGGCAAAGGGCCTCCTACTCAAGGGGGACTTGGA
  TCTGGAGCTGGTGCTGCTGTGTAAGGAGAAGCCCACAACCGCCCTCCTGGACAAGGTGGC
  CGACAACCTGGCCATCCAGCTTGCTGCTGTAACAGAAGACAAGTACGAAATACTGCAATC
  TGTCGACGATGCTGCGATTGTGATAAAAAACACAAAAGAGCCTCCATTGTCCCTGACCAT
  CCACCTGACATCCCCTGTTGTCAGAGAAGAAATGGAGAAAGTATTAGCTGGAGAAACGCT
  ATCAGTCAACGACCCCCCGGACGTTCTGGACAGGCAGAAATGCCTTGCTGCCTTGGCGTC
  CCTCCGACACGCCAAGTGGTTCCAGGCCAGAGCCAACGGGCTGAAGTCTTGTGTCATTGT
  GATCCGGGTCTTGAGGGACCTGTGCACTCGCGTGCCCACCTGGGGTCCCCTCCGAGGCTG
  GCCTCTCGAGCTCCTGTGTGAGAAATCCATTGGCACGGCCAACAGACCGATGGGTGCTGG
  CGAGGCCCTGCGGAGAGTGCTGGAGTGCCTGGCGTCGGGCATCGTGATGCCAGATGGTTC
  TGGCATTTATGACCCTTGTGAAAAAGAAGCCACTGATGCTATTGGGCATCTAGACAGACA
  GCAACGGGAAGATATCACACAGAGTGCGCAGCACGCACTGCGGCTCGCTGCCTTCGGCCA
  GCTCCATAAAGTCCTAGGCATGGACCCTCTGCCTTCCAAGATGCCCAAGAAACCAAAGAA
  TGAAAACCCAGTGGACTACACCGTTCAGATCCCACCAAGCACCACCTATGCCATTACGCC
  CATGAAACGCCCAATGGAGGAGGACGGGGAGGAGAAGTCGCCCAGCAAAAAGAAGAAGAA
  GATTCAGAAGAAAGAGGAGAAGGCAGAGCCCCCCCAGGCTATGAATGCCCTGATGCGGTT
  GAACCAGCTGAAGCCAGGGCTGCAGTACAAGCTGGTGTCCCAGACTGGGCCCGTCCATGC
  CCCCATCTTTACCATGTCTGTGGAGGTTGATGGCAATTCATTCGAGGCCTCTGGGCCCTC
  CAAAAAGACGGCCAAGCTGCACGTGGCCGTTAAGGTGTTACAGGACATGGGCTTGCCGAC
  GGGTGCTGAAGGCAGGGACTCGAGCAAGGGGGAGGACTCGGCTGAGGAGACCGAGGCGAA
  GCCAGCAGTGGTGGCCCCTGCCCCAGTGGTAGAAGCTGTCTCCACCCCTAGTGCGGCCTT
  TCCCTCAGATGCCACTGCCGAGCAGGGGCCGATCCTGACAAAGCACGGCAAGAACCCAGT
  CATGGAGCTGAACGAGAAGAGGCGTGGGCTCAAGTACGAGCTCATCTCCGAGACCGGGGG
  CAGCCACGACAAGCGCTTCGTCATGGAGGTCGAAGTGGATGGACAGAAGTTCCAAGGTGC
  TGGTTCCAACAAAAAGGTGGCGAAGGCCTACGCTGCTCTTGCTGCCCTAGAAAAGCTTTT
  CCCTGACACCCCTCTCGCCCTTGATGCCAACAAAAAGAAGAGAGCCCCAGTACCCGTCAG
  AGGGGGACCGAAATTTGCTGCTAAGCCACATAACCCTGGCTTCGGCATGGGAGGCCCCAT
  GCACAACGAAGTGCCCCCACCCCCCAACCTTCGAGGGCGGGGAAGAGGCGGGAGCATCCG
  GGGACGAGGGCGCGGGCGAGGATTTGGTGGCGCCAACCATGGAGGCTACATGAATGCCGG
  TGCTGGGTATGGAAGCTATGGGTACGGAGGCAACTCGGCGACAGCAGGCTACAGTCAGTT
  CTACAGCAACGGAGGGCATTCTGGGAATGCCAGTGGCGGTGGCGGCGGGGGCGGTGGTGG
  CTCCTCCGGCTATGGCTCCTACTACCAAGGTGACAACTACAACTCACCGGTGCCCCCAAA
  ACACGCTGGGAAGAAGCAGCCGCACGGGGGCCAGCAGAAGCCCTCCTACGGCTCGGGCTA
  CCAGTCCCACCAGGGCCAGCAGCAGTCCTACAACCAGAGCCCCTACAGCAACTATGGCCC
  TCCACAGGGCAAGCAGAAAGGCTATAACCATGGACAAGGCAGCTACTCCTACTCGAACTC
  CTACAACTCTCCCGGGGGCGGGGGCGGATCCGACTACAACTACGAGAGCAAATTCAACTA
  CAGTGGTAGTGGAGGCCGAAGCGGCGGGAACAGCTACGGCTCAGGCGGGGCATCCTACAA
  CCCAGGGTCACACGGGGGCTACGGCGGAGGTTCTGGGGGCGGCTCCTCATACCAAGGCAA
  ACAAGGAGGCTACTCACAGTCGAACTACAACTCCCCGGGGTCCGGCCAGAACTACAGTGG
  CCCTCCCAGCTCCTACCAGTCCTCACAAGGCGGCTATGGCAGAAACGCAGACCACAGCAT
  GAACTACCAGTACAGATAAGCCCCCGCGGGGCGGAGATTTCTACCTTCTGCACTTACTCC
  CCATCAGAAGATCGAGTTTTATGCATCACAGTTAACATGTCAGCTGGCCCTCCAGGCCCC
  CGCCCCCATCCCGTCCACGTTGCTGTGTCGTGAGGTGCAGCGGGTCACCCTGTGGCCCGT
  CCTGTGACCCATATTTAGCCGTGTTTGGGACTCCGTGTCTTCAATGGTTTGTTAGTTGCC
  ATTACAACTTTGTCTGGGTAGAGTTTTTGAGTTTTAACAGTTCAGTATCCCTCTGTCTAT
  TCACACTTAAAGTTAGTGGTAACTCAAATTGTCTTTAAATAGTTACAGAAGGGATACGTC
  ATTTGTTAATGCTTTTGTGAAGTGAGTTAAACGAGCTTTCTGTATTTTAATGCTTTAGTG
  TTTCAGTTTTATAAGTGAAGATTTTATTTTAAAAACCAGTGGGAAAGAGTGGGGGGTTTC
  TT
  
• Restriction Sites: Please inquire
• ACCN: NM_012218
• Insert Size: 3400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_012218.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012218.2, NP_036350.2
• RefSeq Size: 6058 bp
• RefSeq ORF: 2685 bp
• Locus ID: 3609
• UniProt ID: Q12906
• Cytogenetics: 19p13.2
• Domains: DSRM
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (1) uses an alternate splice site in the 3' coding region compared to variant 4. The resulting isoform (a) has a shorter C-terminus compared to isoform d. This isoform is also called NFAR-2, ILF3-E and NF110.