NEDD4 (NM_006154) Human Untagged Clone

CAT#: SC310054

NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDD4
• Synonyms: NEDD4-1; RPF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006154 edited
  CTCCCGCTTCTCCTCCTCCTCCTCCACAGTTGCCTGCCCTGGGCGGGGGCGAGCGCGTCC
  GGTTTGCTGGAAGCGTTCGGAAATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGA
  GGACGAGGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTGCCAA
  GAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTATATGACCCAATGAA
  TGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAAAAAGAGTTTGAATCCAAAGTGGAA
  TGAAGAAATATTATTCAGAGTTCATCCTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGA
  CGAAAACCGATTGACAAGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATT
  ACCGACAGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTCATCC
  AAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATGACTTATTTACCTAA
  AACCAGTGGCTCAGAAGATGATAATGCAGAACAGGCTGAGGAATTAGAGCCTGGCTGGGT
  TGTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACAAGAACCTTCTCCTCT
  ACCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGA
  ATCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAA
  TGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGA
  AACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGA
  AGCCACCATGTATAGCAGCCAGGCCTTCCCATCACCTCCACCGTCAAGTAACTTGGATGT
  TCCAACTCATCTTGCAGAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGT
  GAGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCTATAC
  TTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCACCAGG
  TTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAAC
  GACTACTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAG
  CCAGAGTTCTGCAGGCCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGAC
  CCAGCCATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACC
  AAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACCTGGGAAGATCCAAG
  ATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATACTTCCAATGATCTAGG
  GCCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTACATAAA
  TCACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGG
  ACCAGCAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTT
  GAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCT
  TGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACT
  GTGGATTGAGTTTGATGGTGAAAAGGGATTGGATTATGGAGGAGTTGCCAGAGAATGGTT
  CTTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTAC
  GGACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACCTCTC
  TTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGA
  TGGTTTTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGA
  TATGGAATCTGTGGATAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCC
  AACAGAATTGGACCTCAGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACA
  TGAGCTGAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAATATAT
  TTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAAATGGCTGCTTTTAA
  AGAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAATGAACT
  AGAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAA
  GTATAAAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTT
  AATGATGGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGT
  GCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAGTCATTTACAGTTGA
  ACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCATACCTGTTTTAATCGCCTGGACTT
  GCCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGATGGCAATTGAAAACAC
  CCAGGGCTTTGATGGAGTTGATTAGATTACAAATAACAATCTGTAGTGTTTTTACTGCCA
  TAGTTTTATAACCAAAATCTTGACTTAAAATTTTCCGGGGAACTACTAAAATGTGGCCAC
  TGAGTCTTCCCAGATCTTGAAGAAAATCATATAAAAAGCATTTGAAGAAATAGTACGACA
  ACTTATTTTTAATCACTTTTAAATAATGTGTTGCATTTACACAGTTGTTTCATGCTGTCT
  TTAGAGTTAGGTGCCTGCCTAAAGCCAGGCACCACCACACCTGGCTTTAGAGTTCACACA
  ATAGGATATAAGTCCTGTATGACTTAAATAGTGAATTTTGTCCTTAACATTTACCTCTTG
  TATAGTATCTGCCAGGCAGTTTTTTCTTAAACTACTGAGATGATAACTGTGAAATATTTG
  TGATACGTGTCATGTGTGAAAAGTTTGATGCATTTTGAGATGGAAAACTGAAATTTGGAA
  AAAGAAATACTTTACTATTGAGTAAACTACAATATATTTAGTGCTACTCGCAGCTATTTA
  TTATTTTGTAGACCTGCCTTATGCACCTTACTGCCTAGATTTTTGGGAAAAAACTTTGGA
  AAGTGTGTTACCTATATTTCTAGCCAACTAACTCACAGAAAAACTGTTTACTTCTTCACT
  TTCGAAGTATTTGGCTTTTGTTAATATGCAGTTTTACTAAACAGATGGTTCATAAGACAT
  GTGAAGCAAATTCATATTTGCAATGGGTAAAAAGTATTAAAGCCTTTCTCTTGCCTGCAT
  ATCCTATTGACCATTGGTATAGTCATCACTTTTTCATATTTTAGTGTAGTTAGAAGAATT
  CCTTCTTCAAACATTAAAGATCCACAAAGCAGTATTTCTAAATATGCCTTGAAGAACTAA
  ATGAAGTGTATAGCACTTGCCTTTACTAGATATTCTTTACACTTGTACAATTATGTAGTA
  AATGTATGTTTACAGGGTTTATCATGTTTACAGATTAAGCTAATTTCTGTAGTCGCATTT
  TTATATTTTTAGTATCACTCTAGTAAAAAAACCAAATAATTTGTTTAAAATAACCAAAGA
  GTTGTTATAATGCATAATTTGTATTAAATTTATTACTATTTCTTATGCCTTTTAAAATAC
  TGTTTACTATGAAGACAATGTTTTTAATTACAAATCCAGAATTCTGTAGGCAAAATGCTA
  CAGTTCATATCTTCCTTTAACCAAACTGAAGTACATAAAGACCATGTACATGTATTCATC
  AAACGTTTATTGAATGCTGCGTGCCTAGCGCTGTGCTATGCTCTGGGGTAAGAGTTGTCA
  GCTTCAGAGAAGCTGAGTCCTGATCCTCAAGGAACTTGCAAATGTGTCTATGAATTTGTA
  AAACAATCAAAAGTAGGCGTAAGCAGAATAAGGCAAAAGGGAAAGTGTTCTAGGTTCCAG
  CACACCTGCAAAGATAAAGTGTGCCAAGACTGTATTTATATTTCATACTTATATTGTTTC
  ATCCTTATATTGGAATGATTATATAGAAAATGCTCTTAAAAAGATTAAACCTATTTCTCA
  GTATGGTATCTTGGTGATTTAGGAATAATTGTAAATATATGTTACGAATCTTCTTAAATA
  TATATATATACACACCCTGTGAGAACTGTAAAAAGTACCTCTGGTTCTTGGTTTAAGTTT
  GTTGGGGTATAACATGATGAGTACTCATTAGCACCTGATAGAAATCTGAAATGTGACAGT
  AGCAAAACCACTTTCTACTTTCCAAACACCACAGCATCAGCATGGTTTAGGGGAAGCAAT
  TCACAGATTAATGTACCCTGCGTTTTGTCTTCACCATTGTCAACCAGCAGTCAAGGATGA
  GCACCAGGTATAGTCCTGGTTTGATATCTCACCAGCCATGTGAACTGAAGCAGCTTACTT
  AACCTCTCTGGATTCATATTTCTTCATCTGTCAAATGAGAGTAATCATGCCCACCCTCAT
  TGTCATGGCAGATTGTCAGAAGTTCCAGACAGATGAGAAAGCAAAAAGTACTTTGTAAAG
  TGTTATACAATCAGAAGGTGCTTTGTGCTGACCGTCAGATTGTGTCAAGTCAGGTGTGCA
  AATTGACCACACTCCCAAAGAATCATTTAAAAGTAAAGTGACTTCTAAGAGACAAGGAAG
  TAGGAACATTTCTGATATTAAATATATAAATAATATGGATACACATGTGTATATCTGTAT
  ATGGATCTTACCATCATAATTCATTTTCTTCATATCAGCAAAGTAATAGTAATGTGGGCC
  AATGCATTTTGGAAATGTCCTCATTATGTAGAATGGAATGTGAAAATTATTTTTGTTAAA
  ACAGGATTTTGCCACAATTATTTAAATATTATGTTTGTGAACTATCTAAGCATGAGAAAA
  TACAAAGCTTTCTTTGTATCTGGCTGATACTCATTTGGATGATTCTGTGACTCATAGAAC
  ATGATGTTAAATGAGACCAGACTCAACTGCCACCAGTCCCCAGCTGCAGACCTTCATCCC
  CTTCATCTCCCACTAGGGGCTCGTGGTCTGGAAGAAACATGTATCAAGCAACACAGCCCC
  TTATCCCAGATAGAAAAGTGTCTCAAATGCATTCTACTGCGGGACAGTCAGTAGGATCAT
  TTTCATAAAGCAAGGACATCATATGTTTCTAGAAATTACAAGCATATAACTTTAGCCTAC
  AATCTCTTATTAAAAATTTTTAACAAAATTGTATTACAAATGCATTTCATCAGAACTCAA
  ATTTAAATGGTGTTTGTTTTGGGTTTTTATTTATAATGCTGAAGTTATTCCATATAAGTA
  TCAAGTTAAACACAATTCATTTTGATTATAAACTATTTGACTTTTTAAAATCTTCTGACA
  CAGTAAATATATATATCAAGATTGATGTATCAAAATTTATTGCACACTTTAAAGTGTAAA
  ATCATTTTTTAAAATCTTGAATCCACAAATAAAGTTCTATTCTGATTTTAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_006154
• Insert Size: 5900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 2 SNPs compared with NM_006154.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006154.1, NP_006145.1
• RefSeq Size: 5749 bp
• RefSeq ORF: 2703 bp
• Locus ID: 4734
• UniProt ID: P46934
• Cytogenetics: 15q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis
• Gene Summary: This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains multiple alternate 5' coding exons, compared to variant 2. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (1) is shorter and has a distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.