TJP2 (NM_004817) Human Untagged Clone

CAT#: SC309955

TJP2 (untagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TJP2
• Synonyms: C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1; PFIC4; X104; ZO2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004817 edited
  AGCGGAGGGGCCACGCTCGGGTCGGGGGCGGGCTGACGCCGCCGCCGCCGCGGGAGGAGG
  GACAAAGGGGTGGGTCCCCGCGGGTCGGCACCCCGGCGGTTGGGCTGCGGGTCAGAGCAC
  TGTCCGGTGGTGCCCAGGAGGAGTAGGAGCAGGAGCAGAAGCAGAAGCGGGGTCCGGAGC
  TGCGCGCCTACGCGGGACCTGTGTCCGAAATGCCGGTGCGAGGAGACCGCGGGTTTCCAC
  CCCGGCGGGAGCTGTCAGGTTGGCTCCGCGCCCCAGGCATGGAAGAGCTGATATGGGAAC
  AGTACACTGTGACCCTACAAAAGGATTCCAAAAGAGGATTTGGAATTGCAGTGTCCGGAG
  GCAGAGACAACCCCCACTTTGAAAATGGAGAAACGTCAATTGTCATTTCTGATGTGCTCC
  CGGGTGGGCCTGCTGATGGGCTGCTCCAAGAAAATGACAGAGTGGTCATGGTCAATGGCA
  CCCCCATGGAGGATGTGCTTCATTCGTTTGCAGTTCAGCAGCTCAGAAAAAGTGGGAAGG
  TCGCTGCTATTGTGGTCAAGAGGCCCCGGAAGGTCCAGGTGGCCGCACTTCAGGCCAGCC
  CTCCCCTGGATCAGGATGACCGGGCTTTTGAGGTGATGGACGAGTTTGATGGCAGAAGTT
  TCCGGAGTGGCTACAGCGAGAGGAGCCGGCTGAACAGCCATGGGGGGCGCAGCCGCAGCT
  GGGAGGACAGCCCGGAAAGGGGGCGTCCCCATGAGCGGGCCCGGAGCCGGGAGCGGGACC
  TCAGCCGGGACCGGAGCCGTGGCCGGAGCCTGGAGCGGGGCCTGGACCAAGACCATGCGC
  GCACCCGAGACCGCAGCCGTGGCCGGAGCCTGGAGCGGGGCCTGGACCACGACTTTGGGC
  CATCCCGGGACCGGGACCGTGACCGCAGCCGCGGCCGGAGCATTGACCAGGACTACGAGC
  GAGCCTATCACCGGGCCTACGACCCAGACTACGAGCGGGCCTACAGCCCGGAGTACAGGC
  GCGGGGCCCGCCACGATGCCCGCTCTCGGGGACCCCGAAGCCGCAGCCGCGAGCACCCGC
  ACTCACGGAGCCCCAGCCCCGAGCCTAGGGGGCGGCCGGGGCCCATCGGGGTCCTCCTGA
  TGAAAAGCAGAGCGAACGAAGAGTATGGTCTCCGGCTTGGGAGTCAGATCTTCGTAAAGG
  AAATGACCCGAACGGGTCTGGCAACTAAAGATGGCAACCTTCACGAAGGAGACATAATTC
  TCAAGATCAATGGGACTGTAACTGAGAACATGTCTTTAACGGATGCTCGAAAATTGATAG
  AAAAGTCAAGAGGAAAACTACAGCTAGTGGTGTTGAGAGACAGCCAGCAGACCCTCATCA
  ACATCCCGTCATTAAATGACAGTGACTCAGAAATAGAAGATATTTCAGAAATAGAGTCAA
  ACCGATCATTTTCTCCAGAGGAGAGACGTCATCAGTATTCTGATTATGATTATCATTCCT
  CAAGTGAGAAGCTGAAGGAAAGGCCAAGTTCCAGAGAGGACACGCCGAGCAGATTGTCCA
  GGATGGGTGCGACACCCACTCCCTTTAAGTCCACAGGGGATATTGCAGGCACAGTTGTCC
  CAGAGACCAACAAGGAACCCAGATACCAAGAGGACCCCCCAGCTCCTCAACCAAAAGCAG
  CCCCGAGAACTTTTCTTCGTCCTAGTCCTGAAGATGAAGCAATATATGGCCCTAATACCA
  AAATGGTAAGGTTCAAGAAGGGAGACAGCGTGGGCCTCCGGTTGGCTGGTGGCAATGATG
  TCGGGATATTTGTTGCTGGCATTCAAGAAGGGACCTCGGCGGAGCAGGAGGGCCTTCAAG
  AAGGAGACCAGATTCTGAAGGTGAACACACAGGATTTCAGAGGATTAGTGCGGGAGGATG
  CCGTTCTCTACCTGTTAGAAATCCCTAAAGGTGAAATGGTGACCATTTTAGCTCAGAGCC
  GAGCTGATGTGTATAGAGACATCCTGGCTTGTGGCAGAGGGGATTCGTTTTTTATAAGAA
  GCCACTTTGAATGTGAGAAGGAAACTCCACAGAGCCTGGCCTTCACCAGAGGGGAGGTCT
  TCCGAGTGGTAGACACACTGTATGACGGCAAGCTGGGCAACTGGCTGGCTGTGAGGATTG
  GGAACGAGTTGGAGAAAGGCTTAATCCCCAACAAGAGCAGAGCTGAACAAATGGCCAGTG
  TTCAAAATGCCCAGAGAGACAACGCTGGGGACCGGGCAGATTTCTGGAGAATGCGTGGCC
  AGAGGTCTGGGGTGAAGAAGAACCTGAGGAAAAGTCGGGAAGACCTCACAGCTGTTGTGT
  CTGTCAGCACCAAGTTCCCAGCTTATGAGAGGGTTTTGCTGCGAGAAGCTGGTTTCAAGA
  GACCTGTGGTCTTATTCGGCCCCATAGCTGATATAGCAATGGAAAAATTGGCTAATGAGT
  TACCTGACTGGTTTCAAACTGCTAAAACGGAACCAAAAGATGCAGGATCTGAGAAATCCA
  CTGGAGTGGTCCGGTTAAATACCGTGAGGCAAATTATTGAGCAGGATAAGCATGCACTAC
  TGGATGTGACTCCGAAAGCTGTGGACCTGTTGAATTACACCCAGTGGTTCCCAATTGTGA
  TTTTTTTCAACCCAGACTCCAGACAAGGTGTCAAAACCATGAGACAAAGGTTAAATCCAA
  CGTCCAACAAAAGTTCTCGAAAGTTATTTGATCAAGCCAACAAGCTTAAAAAAACGTGTG
  CACACCTTTTTACAGCTACAATCAACCTAAATTCAGCCAATGATAGCTGGTTTGGCAGCT
  TAAAGGACACTATTCAGCATCAGCAAGGAGAAGCGGTTTGGGTCTCTGAAGGAAAGATGG
  AAGGGATGGATGATGACCCCGAAGACCGCATGTCCTACTTAACCGCCATGGGCGCGGACT
  ATCTGAGTTGCGACAGCCGCCTCATCAGTGACTTTGAAGACACGGACGGTGAAGGAGGCG
  CCTACACTGACAATGAGCTGGATGAGCCAGCCGAGGAGCCGCTGGTGTCGTCCATCACCC
  GCTCCTCGGAGCCGGTGCAGCACGAGGAGAGCATAAGGAAACCCAGCCCAGAGCCACGAG
  CTCAGATGAGGAGGGCTGCTAGCAGCGATCAACTTAGGGACAATAGCCCGCCCCCAGCAT
  TCAAGCCAGAGCCGCCCAAGGCCAAAACCCAGAACAAAGAAGAATCCTATGACTTCTCCA
  AATCCTATGAATATAAGTCAAACCCCTCTGCCGTTGCTGGTAATGAAACTCCTGGGGCAT
  CTACCAAAGGTTATCCTCCTCCTGTTGCAGCAAAACCTACCTTTGGGCGGTCTATACTGA
  AGCCCTCCACTCCCATCCCTCCTCAAGAGGGTGAGGAGGTGGGAGAGAGCAGTGAGGAGC
  AAGATAATGCTCCCAAATCAGTCCTGGGCAAAGTCAAAATATTTGAGAAGATGGATCACA
  AGGCCAGGTTACAGAGAATGCAGGAGCTCCAGGAAGCACAGAATGCAAGGATCGAAATTG
  CCCAGAAGCATCCTGATATCTATGCAGTTCCAATCAAAACGCACAAGCCAGACCCTGGCA
  CGCCCCAGCACACGAGTTCCAGACCCCCTGAGCCACAGAAAGCTCCTTCCAGACCTTATC
  AGGATACCAGAGGAAGTTATGGCAGTGATGCCGAGGAGGAGGAGTACCGCCAGCAGCTGT
  CAGAACACTCCAAGCGCGGTTACTATGGCCAGTCTGCCCGATACCGGGACACAGAATTAT
  AGATGTCTGAGCACGGACTCTCCCAGGCCTGCCTGCATGGCATCAGACTAGCCACTCCTG
  CCAGGCCGCCGGGATGGTTCTTCTCCAGTTAGAATGCACCATGGAGACGTGGTGGGACTC
  CAGCTCGTGTGTCCTCATGGAGAACCCAGGGGACAGCTGGTGCAAATTCAGAACTGAGGG
  CTCTGTTTGTGGGACTGGGTTAGAGGAGTCTGTGGCTTTTTGTTCAGAATTAAGCAGAAC
  ACTGCAGTCAGATCCTGTTACTTGCTTCAGTGGACCGAAATCTGTATTCTGTTTGCGTAC
  TTGTAATATGTATATTAAGAAGCAATAACTATTTTTCCTCATTAATAGCTGCCTTCAAGG
  ACTGTTTCAGTGTGAGTCAGAATGTGAAAAAGGAATAAAAAAATACTGTTGGGCTCAAAC
  TAAATTCAAAGAAGTACTTTATTGCAACTCTTTTAAGTGCCTTGGATGAGAAGTGTCTTA
  AATTTTCTTCCTTTGAAGCTTTAGGCAGAGCCATAATGGACTAAAACATTTTGACTAAGT
  TTTTATACCAGCTTAATAGCTGTAGTTTTCCCTGCACTGTGTCATCTTTTCAAGGCATTT
  GTCTTTGTAATATTTTCCATAATTTTGGACTGTCTATATCATAACTATACTTGATAGTTT
  GGCTATAAGTGCTCAATAGCTTGAAGCCCAAGAAGTTGGTATCGAAATTTGTTGTTTGTT
  TAAACCCAAGTGCTGCACAAAAGCAGATACTTGAGGAAAACACTATTTCCAAAAGCACAT
  GTATTGACAACAGTTTTATAATTTAATAAAAAGGAATACATTACAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004817
• Insert Size: 4600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_004817.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004817.2, NP_004808.2
• RefSeq Size: 4618 bp
• RefSeq ORF: 3573 bp
• Locus ID: 9414
• UniProt ID: Q9UDY2
• Cytogenetics: 9q21.11
• Domains: SH3, PDZ, Guanylate_kin, GuKc
• Protein Pathways: Tight junction, Vibrio cholerae infection
• Gene Summary: This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (1) encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.