NM23A (NME1) (NM_198175) Human Untagged Clone

CAT#: SC309527

NME1 (untagged)-Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 1


  "NM_198175" in other vectors (6)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NM23A
Synonyms AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_198175 edited
ACATTTTTAGGGTGTATTTCTGGCGCTTTAGTCCTGTTTTCTCCTGGACAATTTATGCTT
GCCCCGCACCCCATCGTGCGATTCTCCGCAGTCTTTGGGCTTTGTCTCTCTCTCTTTTTT
TTTTTTTGGAAGTTGCAGAATGGTGATAAATGATTTTCTTTGCTCCTATTGACTGCTAGG
CCCTGTGGCTAGGTACCATAGAGTCTCTACACAGGAGTAAATCAGCCTGGTGTGCAGGGG
AGGCAGACACACAAACAGAAAATTGGACTACAGTGCTAAGATGCTGTAAGAAGAGGTTAA
CTAAAGGACAGGAAGATGGGGCCAAGAGATGGTGCTACTGTCTACTTTAGGGATCGTCTT
TCAAGGCGAGGGGCCTCCTATCTCAAGCTGTGATACAGGAACCATGGCCAACTGTGAGCG
TACCTTCATTGCGATCAAACCAGATGGGGTCCAGCGGGGTCTTGTGGGAGAGATTATCAA
GCGTTTTGAGCAGAAAGGATTCCGCCTTGTTGGTCTGAAATTCATGCAAGCTTCCGAAGA
TCTTCTCAAGGAACACTACGTTGACCTGAAGGACCGTCCATTCTTTGCCGGCCTGGTGAA
ATACATGCACTCAGGGCCGGTAGTTGCCATGGTCTGGGAGGGGCTGAATGTGGTGAAGAC
GGGCCGAGTCATGCTCGGGGAGACCAACCCTGCAGACTCCAAGCCTGGGACCATCCGTGG
AGACTTCTGCATACAAGTTGGCAGGAACATTATACATGGCAGTGATTCTGTGGAGAGTGC
AGAGAAGGAGATCGGCTTGTGGTTTCACCCTGAGGAACTGGTAGATTACACGAGCTGTGC
TCAGAACTGGATCTATGAATGACAGGAGGGCAGACCACATTGCTTTTCACATCCATTTCC
CCTCCTTCCCATGGGCAGAGGACCAGGCTGTAGGAAATCTAGTTATTTACAGGAACTTCA
TCATAATTTGGAGGGAAGCTCTTGGAGCTGTGAGTTCTCCCTGTACAGTGTTACCATCCC
CGACCATCTGATTAAAATGCTTCCTCCCAGCAAAAAAAAAAAAAAAAAA
Restriction Sites NotI-NotI     
ACCN NM_198175
Insert Size 1100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone was fully sequenced and found to be a perfect match to NM_198175.1.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_198175.1, NP_937818.1
RefSeq Size 1031 bp
RefSeq ORF 534 bp
Locus ID 4830
UniProt ID P15531
Cytogenetics 17q21.33
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Metabolic pathways, Purine metabolism, Pyrimidine metabolism
Gene Summary This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

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