KCNQ2 (NM_172107) Human Untagged Clone

CAT#: SC309163

KCNQ2 (untagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNQ2
• Synonyms: BFNC; DEE7; EBN; EBN1; ENB1; HNSPC; KCNA11; KV7.2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172107 edited
  CCCCGCTGAGCCTGAGCCCGACCCGGGGCGCCTCCCGCCAGGCACCATGGTGCAGAAGTC
  GCGCAACGGCGGCGTATACCCCGGCCCGAGCGGGGAGAAGAAGCTGAAGGTGGGCTTCGT
  GGGGCTGGACCCCGGCGCGCCCGACTCCACCCGGGACGGGGCGCTGCTGATCGCCGGCTC
  CGAGGCCCCCAAGCGCGGCAGCATCCTCAGCAAACCTCGCGCGGGCGGCGCGGGCGCCGG
  GAAGCCCCCCAAGCGCAACGCCTTCTACCGCAAGCTGCAGAATTTCCTCTACAACGTGCT
  GGAGCGGCCGCGCGGCTGGGCGTTCATCTACCACGCCTACGTGTTCCTCCTGGTTTTCTC
  CTGCCTCGTGCTGTCTGTGTTTTCCACCATCAAGGAGTATGAGAAGAGCTCGGAGGGGGC
  CCTCTACATCCTGGAAATCGTGACTATCGTGGTGTTTGGCGTGGAGTACTTCGTGCGGAT
  CTGGGCCGCAGGCTGCTGCTGCCGGTACCGTGGCTGGAGGGGGCGGCTCAAGTTTGCCCG
  GAAACCGTTCTGTGTGATTGACATCATGGTGCTCATCGCCTCCATTGCGGTGCTGGCCGC
  CGGCTCCCAGGGCAACGTCTTTGCCACATCTGCGCTCCGGAGCCTGCGCTTCCTGCAGAT
  TCTGCGGATGATCCGCATGGACCGGCGGGGAGGCACCTGGAAGCTGCTGGGCTCTGTGGT
  CTATGCCCACAGCAAGGAGCTGGTCACTGCCTGGTACATCGGCTTCCTTTGTCTCATCCT
  GGCCTCGTTCCTGGTGTACTTGGCAGAGAAGGGGGAGAACGACCACTTTGACACCTACGC
  GGATGCACTCTGGTGGGGCCTGATCACGCTGACCACCATTGGCTACGGGGACAAGTACCC
  CCAGACCTGGAACGGCAGGCTCCTTGCGGCAACCTTCACCCTCATCGGTGTCTCCTTCTT
  CGCGCTGCCTGCAGGCATCTTGGGGTCTGGGTTTGCCCTGAAGGTTCAGGAGCAGCACAG
  GCAGAAGCACTTTGAGAAGAGGCGGAACCCGGCAGCAGGCCTGATCCAGTCGGCCTGGAG
  ATTCTACGCCACCAACCTCTCGCGCACAGACCTGCACTCCACGTGGCAGTACTACGAGCG
  AACGGTCACCGTGCCCATGTACAGTTCGCAAACTCAAACCTACGGGGCCTCCAGACTTAT
  CCCCCCGCTGAACCAGCTGGAGCTGCTGAGGAACCTCAAGAGTAAATCTGGACTCGCTTT
  CAGGAAGGACCCCCCGCCGGAGCCGTCTCCAAGTAAAGGCAGCCCGTGCAGAGGGCCCCT
  GTGTGGATGCTGCCCCGGACGCTCTAGCCAGAAGGTCAGTTTGAAAGATCGTGTCTTCTC
  CAGCCCCCGAGGCGTGGCTGCCAAGGGGAAGGGGTCCCCGCAGGCCCAGACTGTGAGGCG
  GTCACCCAGCGCCGACCAGAGCCTCGAGGACAGCCCCAGCAAGGTGCCCAAGAGCTGGAG
  CTTCGGGGACCGCAGCCGGGCACGCCAGGCTTTCCGCATCAAGGGTGCCGCGTCACGGCA
  GAACTCAGAAGAAGCAAGCCTCCCCGGAGAGGACATTGTGGATGACAAGAGCTGCCCCTG
  CGAGTTTGTGACCGAGGACCTGACCCCGGGCCTCAAAGTCAGCATCAGAGCCGTGTGTGT
  CATGCGGTTCCTGGTGTCCAAGCGGAAGTTCAAGGAGAGCCTGCGGCCCTACGACGTGAT
  GGACGTCATCGAGCAGTACTCAGCCGGCCACCTGGACATGCTGTCCCGAATTAAGAGCCT
  GCAGTCCAGAGTGGACCAGATCGTGGGGCGGGGCCCAGCGATCACGGACAAGGACCGCAC
  CAAGGGCCCGGCCGAGGCGGAGCTGCCCGAGGACCCCAGCATGATGGGACGGCTCGGGAA
  GGTGGAGAAGCAGGTCTTGTCCATGGAGAAGAAGCTGGACTTCCTGGTGAATATCTACAT
  GCAGCGGATGGGCATCCCCCCGACAGAGACCGAGGCCTACTTTGGGGCCAAAGAGCCGGA
  GCCGGCGCCGCCGTACCACAGCCCGGAAGACAGCCGGGAGCATGTCGACAGGCACGGCTG
  CATTGTCAAGATCGTGCGCTCCAGCAGCTCCACGGGCCAGAAGAACTTCTCGGCGCCCCC
  GGCCGCGCCCCCTGTCCAGTGTCCGCCCTCCACCTCCTGGCAGCCACAGAGCCACCCGCG
  CCAGGGCCACGGCACCTCCCCCGTGGGGGACCACGGCTCCCTGGTGCGCATCCCGCCGCC
  GCCTGCCCACGAGCGGTCGCTGTCCGCCTACGGCGGGGGCAACCGCGCCAGCATGGAGTT
  CCTGCGGCAGGAGGACACCCCGGGCTGCAGGCCCCCCGAGGGGAACCTGCGGGACAGCGA
  CACGTCCATCTCCATCCCGTCCGTGGACCACGAGGAGCTGGAGCGTTCCTTCAGCGGCTT
  CAGCATCTCCCAGTCCAAGGAGAACCTGGATGCTCTCAACAGCTGCTACGCGGCCGTGGC
  GCCTTGTGCCAAAGTCAGGCCCTACATTGCGGAGGGAGAGTCAGACACCGACTCCGACCT
  CTGTACCCCGTGCGGGCCCCCGCCACGCTCGGCCACCGGCGAGGGTCCCTTTGGTGACGT
  GGGCTGGGCCGGGCCCAGGAAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172107 unedited
  GTTAAATTTGTATACCACTCATATAGGGCGGCCGCGAATTCGCACGAGCCCCGCTGAGCC
  TGAGCCCGACCCGGGGCGCCTCCCGCCAGGCACCATGGTGCAGAAGTCGCGCAACGGCGG
  CGTATACCCCGGCCCGAGCGGGGAGAAGAAGCTGAAGGTGGGCTTCGTGGGGCTGGACCC
  CGGCGCGCCCGACTCCACCCGGGACGGGGCGCTGCTGATCGCCGGCTCCGAGGCCCCCAA
  GCGCGGCAGCATCCTCAGCAAACCTCGCGCGGGCGGCGCGGGCGCCGGGAAGCCCCCCAA
  GCGCAACGCCTTCTACCGCAAGCTGCAGAATTTCCTCTACAACGTGCTGGAGCGGCCGCG
  CGGCTGGGCGTTCATCTACCACGCCTACGTGTTCCTCCTGGTTTTCTCCTGCCTCGTGCT
  GTCTGTGTTTTCCACCATCAAGGAGTATGAGAAGAGCTCGGAGGGGGCCCTCTACATCCT
  GGAAATCGTGACTATCGTGGTGTTTGGCGTGGAGTACTTCGTGCGGATCTGGGCCGCAGG
  CTGCTGCTGCCGGTACCGTGGCTGGAGGGGGCGGCTCAAGTTTGCCCGGAAACCGTTCTG
  TGTGATTGACATCATGGTGCTCATCGCCTCCATTGCGGTGCTGGCCGCCGGCTCCCAGGG
  CAACGTCTTTGCCACATCTGCGCTCCGGAGCCTGCGCTTCCTGCAGATTCTGCGGATGAT
  CCGCATGGACCGGCGGGGAGGCACCTGGAAGCTGCTGGGCTCTGTGGTCTATGCCCACAG
  CAAGGAGCTGGTCACTGCCTGGTACATCGGCTTCCTTTGTCTCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172107 unedited
  CATGGGGATGGCACTTCCGGCCAGGAAGCACTGGGGAGGGTCACAGGGTGCCCCCGGGAT
  CTGTTCAGAAACAGCTATGACCGCGGCCGCAATCTAGATCACTTCCTGGGCCCGGCCCAG
  CCCACGTCACCAAAGGGACCCTCGCCGGTGGCCGAGCGTGGCGGGGGCCCGCACGGGGTA
  CAGAGGTCGGAGTCGGTGTCTGACTCTCCCTCCGCAATGTAGGGCCTGACTTTGGCACAA
  GGCGCCACGGCCGCGTAGCAGCTGTTGAGAGCATCCAGGTTCTCCTTGGACTGGGAGATG
  CTGAAGCCGCTGAAGGAACGCTCCAGCTCCTCGTGGTCCACGGACGGGATGGAGATGGAC
  GTGTCGCTGTCCCGCAGGTTCCCCTCGGGGGGCCTGCAGCCCGGGGTGTCCTCCTGCCGC
  AGGAACTCCATGCTGGCGCGGTTGCCCCCGCCGTAGGCGGACAGCGACCGCTCGTGGGCA
  GGCGGCGGCGGGATGCGCACCAGGGAGCCGTGGTCCCCCACGGNGGAGGTGCCGTGGCCC
  TGGCGCGGGTGGCTCTGTGGCTGCCAGGAGGTGGAGGGCGGACACTGGACAGGGGGCGCG
  GCCGGNGGCGCCGAGAAGTTCTTCTGGCCCGTGGAGCTGCTGGAGCGCACGATCTTGACA
  ATGCAGCCGTGCCTGTCGACATGCTCCCGGCTGTCTTCCGGGCTGTGGTACGGCGGCGCC
  GGCTCGGGCTCTTTGGCCCCAAAGTAGGCCTCGGTCTCTGTCGGGGGGATGCCCATCCGC
  TGCATGTANATATTCACCAGGAGTCCAGCTCTTTCTCATGGACAAGACTGCTTCTA
  
• Restriction Sites: Please inquire
• ACCN: NM_172107
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172107.2, NP_742105.1
• RefSeq Size: 3251 bp
• RefSeq ORF: 2619 bp
• Locus ID: 3785
• UniProt ID: O43526
• Cytogenetics: 20q13.33
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: The M channel is a slowly activating and deactivating potassium channel that plays a critical role in the regulation of neuronal excitability. The M channel is formed by the association of the protein encoded by this gene and a related protein encoded by the KCNQ3 gene, both integral membrane proteins. M channel currents are inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 1 (BFNC), also known as epilepsy, benign neonatal type 1 (EBN1). At least five transcript variants encoding five different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.