CHD1 (NM_001270) Human Untagged Clone

CAT#: SC309044

CHD1 (untagged)-Human chromodomain helicase DNA binding protein 1 (CHD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHD1
• Synonyms: CHD-1; PILBOS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001270 edited
  CTCGCTTGCTTTCTCGGCGTCATGGCGGCTGCCGGGGGCAGATAAGCGGAGGGAGCGGGA
  GCGCGCGCGCGACGGCGGCGGCGGCGGCGGCGACGACTGGTTACTTATAGCTCTTGCTGC
  CCTCGCCCTTGGTGCTTCAATAATGAATTGTTAGCTCCACTCCGCAGGGGATCGCGCGTT
  GGTGCTGCGGACCGGGGCTTCCCCTTCCCCTCCGCGTCTGTCCCCTTCCCCCTCCCCCGC
  ACGGACTCTTGCTTACCCCGAGACGCCGGAGCCCTAGGACTGGGCGGCGGAGATCCCGGG
  CCTGGGCGCGGGGGAGGCCGTCCACCCGAGTCGGCTCCCTCCGCCCCGCCAAGCCCGCCG
  GTGAATCAAAGGAGAGTCCCAGAAAACCTGTGACTGTTGAAGAAAATTCATCTGTGAATT
  TTTATATTCAAGGAGTCAGTATTTATATTCATCTTTTAAACTGGGAAGATTTATATTTTA
  CTTTAAAACTTCTTGATAATAATTTACAATGAATGGACACAGTGATGAAGAAAGTGTTAG
  AAACAGTAGTGGAGAATCAAGCCAGTCGGATGATGATTCTGGGTCAGCTTCAGGCTCTGG
  ATCTGGTTCGAGTTCTGGAAGCAGTAGTGATGGAAGCAGTAGCCAGTCAGGTAGCAGTGA
  CTCTGACTCCGGATCTGAATCAGGCAGTCAGTCAGAGTCTGAGTCAGACACTTCCCGAGA
  AAACAAAGTTCAAGCAAAACCACCGAAAGTTGATGGAGCTGAGTTTTGGAAATCTAGTCC
  TAGTATTCTGGCCGTTCAGAGATCTGCAATCCTCAAGAAGCAGCAACAGCAGCAGCAGCA
  ACAACAACATCAAGCCTCATCTAATAGCGGATCAGAAGAGGATTCCTCTAGCAGTGAAGA
  TTCCGATGACTCATCAAGTGAGGTCAAAAGGAAAAAGCATAAAGATGAAGATTGGCAAAT
  GTCTGGGTCAGGATCTCCATCTCAGTCTGGTTCAGATTCAGAATCTGAAGAAGAGAGAGA
  GAAAAGCAGTTGTGATGAAACAGAATCTGATTATGAGCCAAAAAACAAAGTCAAAAGCAG
  AAAACCTCAAAATAGATCTAAGTCAAAAAATGGAAAGAAGATTCTTGGACAAAAAAAGAG
  ACAGATTGATTCATCTGAGGAGGATGATGATGAAGAAGATTATGATAATGATAAAAGAAG
  TTCTCGTCGCCAAGCAACTGTTAATGTTAGCTATAAGGAGGATGAAGAAATGAAAACAGA
  TTCTGATGACCTACTGGAAGTCTGTGGAGAGGATGTTCCTCAACCTGAGGAAGAGGAATT
  TGAAACCATAGAAAGATTTATGGATTGTCGGATTGGGAGAAAAGGAGCTACTGGTGCTAC
  TACAACCATCTATGCAGTTGAAGCAGATGGTGACCCAAATGCAGGCTTTGAAAAAAACAA
  AGAACCAGGAGAGATTCAGTATTTAATTAAATGGAAAGGATGGTCCCATATCCACAACAC
  TTGGGAGACAGAAGAAACCCTCAAGCAGCAGAATGTTAGAGGAATGAAAAAATTGGATAA
  TTATAAGAAAAAAGATCAGGAAACAAAAAGATGGTTGAAAAATGCCTCTCCAGAAGATGT
  GGAATATTATAATTGCCAGCAAGAACTTACAGATGATCTACATAAACAGTATCAAATAGT
  GGAACGTATAATTGCTCATTCCAATCAAAAGTCAGCAGCTGGTTATCCTGATTATTACTG
  CAAATGGCAGGGCCTTCCATACTCAGAGTGCAGCTGGGAAGATGGAGCTCTCATTTCCAA
  AAAGTTTCAAGCATGCATTGATGAGTATTTTAGCAGGAACCAATCAAAAACCACTCCTTT
  TAAAGATTGCAAAGTATTAAAACAAAGGCCAAGGTTTGTAGCCCTGAAGAAGCAGCCATC
  CTATATTGGAGGACATGAGGGCTTAGAATTAAGAGATTATCAACTGAATGGTTTAAATTG
  GCTTGCTCATTCTTGGTGCAAAGGAAATAGTTGCATACTCGCTGATGAAATGGGCCTTGG
  AAAAACAATACAGACGATCTCATTTCTGAATTATTTGTTTCATGAACATCAATTATATGG
  ACCTTTTTTATTGGTAGTACCGCTCTCCACTCTTACTTCCTGGCAAAGGGAAATTCAGAC
  TTGGGCTTCTCAAATGAATGCTGTGGTTTATTTAGGTGACATTAACAGCAGAAACATGAT
  AAGAACTCATGAATGGACGCATCATCAGACCAAACGGTTAAAATTTAATATATTGTTAAC
  AACTTATGAAATTTTATTAAAAGATAAGGCATTCCTTGGAGGTCTAAATTGGGCATTTAT
  AGGTGTTGATGAAGCACACCGATTAAAGAATGATGACTCCCTTCTGTATAAAACTTTAAT
  AGATTTTAAATCCAATCATCGTCTCCTTATCACTGGAACTCCTCTACAGAATTCCCTCAA
  AGAGCTCTGGTCTTTGCTACATTTCATTATGCCAGAAAAGTTTTCTTCCTGGGAAGATTT
  TGAAGAAGAACATGGCAAAGGGAGAGAATATGGTTATGCAAGCCTTCACAAGGAGCTTGA
  GCCATTTCTGTTACGCCGAGTTAAGAAAGATGTGGAAAAATCTCTTCCTGCCAAGGTTGA
  GCAGATTTTAAGAATGGAAATGAGTGCTTTACAGAAACAATATTACAAATGGATTTTAAC
  TAGGAATTACAAAGCCCTCAGCAAAGGTTCCAAGGGCAGTACCTCAGGCTTTTTGAACAT
  TATGATGGAGCTAAAGAAATGTTGTAACCATTGCTACCTCATTAAACCACCAGATAATAA
  TGAATTCTATAATAAACAGGAGGCCTTACAACACTTAATTCGTAGTAGCGGAAAATTGAT
  TCTTCTTGACAAGCTATTAATTCGCCTAAGAGAACGAGGCAATCGAGTTCTTATTTTTTC
  ACAAATGGTGCGGATGTTAGATATACTTGCAGAATATTTGAAATATCGTCAATTCCCCTT
  TCAAAGATTAGATGGATCAATAAAAGGAGAACTGAGGAAACAAGCTCTAGATCATTTTAA
  TGCTGAGGGATCAGAGGATTTTTGCTTTTTGCTGTCCACAAGAGCTGGAGGTCTAGGGAT
  TAATTTAGCCTCTGCTGACACTGTTGTTATATTTGATTCCGATTGGAATCCACAGAATGA
  TCTTCAGGCACAGGCTAGAGCCCATCGAATTGGGCAAAAGAAACAGGTGAATATTTATCG
  TCTAGTTACAAAGGGATCAGTTGAAGAAGATATTCTTGAAAGGGCGAAAAAGAAGATGGT
  TTTAGATCATCTTGTAATTCAAAGAATGGACACAACTGGGAAGACAGTACTACATACAGG
  TTCTGCCCCATCAAGTTCTACTCCTTTCAATAAAGAAGAGTTATCAGCCATTTTAAAGTT
  TGGTGCTGAAGAACTTTTTAAGGAACCTGAAGGAGAAGAACAAGAGCCCCAGGAAATGGA
  TATAGATGAAATCTTGAAGAGAGCTGAAACTCATGAAAATGAACCAGGTCCTTTAACTGT
  AGGAGATGAATTGCTTTCCCAGTTCAAGGTTGCCAACTTCTCAAATATGGATGAGGATGA
  CATTGAGTTGGAACCTGAAAGAAATTCAAAGAATTGGGAGGAAATTATTCCAGAAGATCA
  AAGAAGACGATTAGAAGAAGAAGAAAGACAAAAGGAACTTGAAGAAATTTATATGCTCCC
  AAGAATGAGAAATTGTGCAAAACAGATTAGTTTCAATGGAAGTGAAGGGAGGCGCAGTAG
  AAGTAGGAGATACTCTGGATCTGATAGTGATTCCATCTCAGAAGGGAAAAGGCCAAAGAA
  ACGTGGAAGACCACGGACTATTCCTCGGGAGAATATTAAAGGATTTAGTGATGCAGAAAT
  TAGGCGGTTTATCAAGAGCTATAAGAAATTTGGTGGTCCTCTGGAAAGATTAGATGCAAT
  TGCTCGAGATGCTGAGTTAGTTGATAAGTCAGAAACAGACCTTAGACGACTGGGAGAATT
  GGTACATAATGGTTGCATTAAAGCATTAAAGGATAGTTCTTCAGGAACAGAACGAACAGG
  TGGTAGACTCGGAAAAGTGAAGGGTCCAACATTCCGAATATCAGGAGTACAGGTGAATGC
  CAAACTAGTCATCTCCCATGAAGAAGAATTAATACCTTTGCACAAATCCATTCCTTCTGA
  TCCAGAAGAAAGAAAGCAGTATACTATCCCATGCCACACAAAGGCAGCTCATTTTGATAT
  AGACTGGGGCAAAGAAGATGATTCCAATTTGTTAATTGGCATCTATGAATATGGATATGG
  AAGCTGGGAAATGATTAAAATGGATCCTGACCTCAGTCTAACACACAAGATTCTTCCAGA
  TGATCCCGATAAAAAACCACAAGCAAAACAGTTGCAGACCCGTGCAGACTACCTCATCAA
  ATTACTTAGTAGAGATCTTGCAAAAAAAGAAGCTCTTTCTGGTGCGGGAAGTTCAAAGAG
  GAGAAAAGCAAGAGCTAAGAAGAATAAAGCAATGAAGTCTATAAAAGTGAAAGAGGAAAT
  AAAGAGTGATTCTTCTCCTCTGCCTTCAGAGAAGTCTGATGAAGATGATGATAAATTGAG
  TGAATCCAAGTCTGATGGTAGGGAAAGATCCAAGAAATCTTCAGTGTCAGATGCTCCAGT
  TCATATCACGGCAAGTGGTGAACCAGTTCCCATTTCTGAAGAATCTGAAGAGCTGGATCA
  GAAGACATTCAGCATTTGTAAAGAAAGAATGAGGCCTGTTAAAGCAGCTTTGAAACAACT
  TGATAGGCCTGAGAAAGGCCTTTCAGAAAGAGAACAACTAGAACATACTAGACAATGTTT
  AATAAAAATTGGAGACCATATCACAGAATGTCTAAAAGAGTATACAAATCCTGAACAAAT
  TAAGCAATGGAGAAAAAACCTGTGGATTTTTGTATCTAAGTTTACTGAATTTGATGCAAG
  AAAATTACATAAATTATATAAGCATGCTATTAAAAAACGGCAGGAGTCTCAGCAAAACAG
  TGATCAAAACAGCAACTTGAATCCTCACGTGATTAGAAATCCAGATGTGGAAAGATTAAA
  AGAGAATACAAATCACGATGATAGCAGCAGGGACAGTTATTCCTCTGATAGACACTTAAC
  TCAGTACCATGATCATCATAAAGACCGACATCAGGGAGATTCTTACAAAAAAAGTGATTC
  CAGGAAAAGACCCTATTCTTCTTTTAGTAATGGTAAAGACCATCGTGATTGGGATCACTA
  CAAGCAAGACAGCAGATATTACAGTGACAGAGAGAAACACAGAAAACTGGATGATCACAG
  GAGTAGAGATCACAGGTCAAATTTGGAAGGAAGTTTAAAAGATAGATCTCATTCTGATCA
  TCGTTCTCACTCAGATCATCGGTTACATTCAGACCACCGGTCAAGTTCTGAATATACGCA
  CCATAAATCTTCCAGGGATTATAGGTATCACTCAGACTGGCAAATGGACCACAGAGCTTC
  CAGCAGTGGCCCTAGGTCACCACTAGATCAGAGA::T:CTTATGGCTCCAGATCTCCATT
  TGAACATTCAGTTGAACACAAAAGTACACCGGAGCATACCTGGAGTAGTCGGAAAACATA
  ACAAAAACTGATACTTCGTCTTTCTGGACTTTTCTTTTAGCCATATATCATAAACCAACA
  CAGTAATTGCCTTACATGACTTGAAAGATATAAACAGATCTTCTATCAGTAGCAGTATTG
  TTACTTCTTTCCAGGATGCAAGGTCTATTATCCCAACAGAAGAGAAAATATTTTTATATT
  TAAGGATTATGCTGCACTGTACTACAAAATTGTAGTACTTTTTTTTGTTTTCTTTTTTAA
  AGAAATGGAAAATGTTTACTATTACAGGGACCTCAACACTGCCCTCCCATACAGGCTGGA
  TAAAACTGTTTTTAAGTCAGTGATTTTAGACTGACCTCCATTTAAATTATGTTTATATAT
  GAACTTTACTCTGACCTGTGATCATGTTTCAGGAAGGAATGAAAGAGAGTTCTTTCTTAA
  TAAAGAAAAACACTCAAGGACTTTGTTCATTTCCAAAGCTACTTGTTTACATTGTACACT
  GCGACCACCTTGCCGCTTTTCATCACAAGCTTGAATATTTAAATTCTGTACTTATATCTG
  TAAAATAGCCAGGAATTTCCTGTTTGTGATCTATTATGCCTTTTTACAAAAAAAATGGCT
  GTAAATTATTGTAAATATTAAAGGAACTTTCCTTACTTCCTTCCCTTTCTCAGGCTTTTT
  TTGACTGTTCCTTTCCCTACCAACTCAGGCCTTCTTATTAAAAAAAAAAAAATCAGTGTA
  ATAACACTTTTTAATGATTTGTCTTGATGGAATCATTGTTTAGAATGTAAAAATGGGGAA
  AGGGGCCACTTAATTCCATTAGTCCTCTTTTTATACTGAATATTTTATTAGATACATGTT
  ATTCCCTTTTTTTTCCTTTTTTAGTCAATATTGTGTTTGTAGTTTTAAAAAATGGCGAGA
  TATGTAAAATCTAAACTGCATGCTCTGGAAACACTTTTTTCAGATGCATCTGGTTTAAAA
  GGGTAGGTGTATAAACACTTTTCAGAATCCAAAACGGCCAAAAGTTATTGTAAATCCGTT
  TGTTTTCCCGTTTTATGTGGGCAATAATGTCAAATGTGCTATGCAGCCAGGTTAACATTT
  TAGATAAACTTGATTGACTTTTAATATAAACTGTTACAATGCACACTGATTGTATATAAA
  AACGTTATATATGACAAATTAAATTTAAGAAAAAGGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001270 unedited
  AGTACAACGCTCTTGAATCCTAACGTGAATTAGTAAATCTCTGATGTGGAAAGATTAAAA
  GAGAATACAAATCACGATGATAGCAGCAGGGACAGTTATTCCTCTGATAGACACTTAACT
  CAGTACCATGATCATCATAAAGACCGACATCAGGGAGATTCTTACAAAAAAAGTGATTCC
  AGGAAAAGACCCTATTCTTCTTTTAGTAATGGTAAAGACCATCGTGATTGGGATCACTAC
  AAGCAAGACAGCAGATATTACAGTGACAGAGAGAAACACAGAAAACTGGATGATCACAGG
  AGTAGAGATCACAGGTCAAATTTGGAAGGAAGTTTAAAAGATAGATCTCATTCTGATCAT
  CGTTCTCACTCAGATCATCGGTTACATTCAGACCACCGGTCAAGTTCTGAATATACGCAC
  CATAAATCTTCCAGGGATTATAGGTATCACTCAGACTGGCAAATGGACCACAGAGCTTCC
  AGCAGTGGCCCTAGGTCACCACTAGATCAGAGATCTTATGGCTCCAGATCTCCATTTGAA
  CATTCAGTTGAACACAAAAGTACACCGGAGCATACCTGGAGTAGTCGGAAAACATAACAA
  AAACTGATACTTCGTCTTTCTGGACTTTTCTTTTAGCCATATATCATAAACCAACACAGT
  AATTGCCTTACATGACTTTGAAAGATATAAACAGATCTTCTATCAGTAGCAGTATTGTTA
  CTTCTTTCCAGGATGCAAGGTCTATTATCCCAACAGAAGAGAAAATATTTTTATATTTAA
  GGATTATGCTGCACTGTACTACAAATTGTAGTACTTTTTTTTGGTTTTCTTTTTTAAAGA
  ATGGAAAATGTTTACTATTACAGGGACCTCACACTGCCCTCCCATACAGGCTGGATAAAA
  ACTGTTTTTTAAGTCAGTGATTTAGAACTGACCCTCCATTTT
  
• Restriction Sites: Please inquire
• ACCN: NM_001270
• Insert Size: 7000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to have a single amino acid difference from the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270.2, NP_001261.2
• RefSeq Size: 6457 bp
• RefSeq ORF: 5133 bp
• Locus ID: 1105
• UniProt ID: O14646
• Cytogenetics: 5q15-q21.1
• Domains: SNF2_N, CHROMO, DEAD, helicase_C
• Protein Families: Druggable Genome
• Gene Summary: The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.