EYA4 (NM_004100) Human Untagged Clone

CAT#: SC308997

EYA4 (untagged)-Human eyes absent homolog 4 (Drosophila) (EYA4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EYA4
• Synonyms: CMD1J; DFNA10
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004100 edited
  GGGAAAAGCCGGGGAGTGACGACTGCGGCGGCTGGGCGCGCTCTCTCATTTTCTTTTCTT
  CTCCTTTCCCCCCTGTCGCAGTCCGGAGTTTTGGCTCCTCTCCTTTCCTCCTCCCCCTCG
  GAGCCGGCTTCTCCCTCCGCCCCGCTTCTCCCCCGCTTGTGTACGCTATTTGTTGTGGGG
  TGGCCGAAGGGGATGTCCTGTTTTCACCAGAGGCACAGCGCGAAGGGGAAACTTCGACAC
  TGGAAGGAACGAGAATAAATACTTAATTACGGACGCACTGAACCGCGGCTGGGACAGACA
  CTTCGGGAACCCGAGGCGGACCGGGCGACGAGATAGTCATTTTTACTTGAAGGAAGCTGC
  TTCTACTTGGGAGTGGCAGGAGAAGTGAGAAAACCACATGGAAGACTCCCAGGATTTAAA
  TGAACAATCAGTAAAGAAAACGTGCACAGAATCAGATGTTTCACAATCTCAGAATTCCAG
  GTCTATGGAAATGCAGGACCTAGCAAGTCCTCATACTCTTGTTGGAGGTGGTGATACTCC
  AGGTAGCTCCAAACTGGAAAAATCTAATCTCAGCAGCACATCAGTTACTACAAATGGGAC
  AGGAGGGGAAAACATGACTGTTTTAAACACAGCAGACTGGTTGCTGAGTTGCAACACCCC
  CTCTTCTGCAACAATGTCTCTTCTTGCAGTCAAAACAGAGCCCTTGAACAGCAGTGAAAC
  CACAGCCACGACTGGAGATGGAGCGCTTGACACTTTTACTGGGTCAGTAATTACAAGTAG
  TGGCTACAGCCCCAGATCAGCACATCAGTATTCCCCACAGCTGTATCCTTCCAAGCCCTA
  TCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCTGCCTATGCAGGCCAGACTCA
  GTATTCGGGGATGCAGCAGCCAGCCGTCTACACAGCCTACTCACAGACAGGACAGCCCTA
  CAGCTTGCCCACTTACGATTTGGGTGTGATGTTGCCAGCCATCAAGACAGAGAGTGGACT
  TTCCCAAACTCAGTCCCCATTACAGAGTGGCTGCCTCAGTTACAGCCCAGGGTTCTCTAC
  CCCACAGCCAGGCCAGACACCTTATTCTTACCAAATGCCAGGTTCTAGTTTTGCACCATC
  ATCTACTATTTATGCAAATAATTCAGTTTCCAATTCAACAAATTTCAGTGGTTCACAACA
  GGATTATCCATCCTATACAGCCTTTGGCCAAAACCAGTATGCACAGTATTATTCAGCATC
  AACGTATGGAGCGTATATGACATCGAATAACACAGCCGATGGCACACCCTCTTCAACCTC
  TACTTATCAGTTGCAGGAATCTCTCCCAGGACTGACTAACCAACCAGGAGAGTTCGATAC
  CATGCAGAGTCCCTCCACACCCATCAAAGATCTTGATGAGAGAACCTGTAGGAGTTCTGG
  GTCAAAGTCCAGAGGAAGAGGCCGGAAAAATAATCCCTCCCCGCCTCCTGATAGTGACCT
  GGAGCGTGTGTTTGTCTGGGATTTGGATGAAACCATCATTGTTTTTCACTCACTGCTCAC
  CGGGTCTTATGCACAGAAGTATGGCAAGGATCCCCCCATGGCTGTAACCCTTGGACTCCG
  CATGGAAGAAATGATTTTTAATCTTGCTGATACTCATTTGTTTTTTAATGATTTAGAGGA
  GTGTGATCAAGTTCATATAGATGATGTTTCCTCTGATGATAATGGGCAGGACTTAAGTAC
  CTACAGTTTTGCAACTGATGGCTTCCATGCAGCTGCAAGTAGTGCAAACCTTTGTTTGCC
  AACAGGTGTAAGAGGAGGGGTTGACTGGATGAGGAAGTTGGCTTTTCGTTACAGAAGAGT
  AAAAGAATTATATAACACCTACAAGAACAACGTTGGAGGACTCCTTGGCCCTGCCAAGAG
  GGATGCCTGGCTACAGTTAAGGGCAGAGATTGAAGGTCTGACAGATTCCTGGCTAACAAA
  TGCACTTAAGTCTTTATCAATTATTAGCACTAGGAGTAACTGCATAAATGTCTTGGTAAC
  GACAACTCAACTGATCCCAGCACTTGCGAAGGTTCTACTCTATAGTTTAGGAGGTGCTTT
  CCCCATTGAGAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGTTGCTTTGAACGAAT
  AATGCAAAGGTTTGGCAGAAAAGTAGTGTATGTTGTAATTGGGGATGGTGTAGAAGAAGA
  ACAGGCAGCAAAAAAGCACAACATGCCCTTCTGGAGGATATCCAGTCACTCAGACCTCCT
  GGCTCTCCACCAAGCACTGGAATTAGAGTATTTGTAACTGTGTTCTTTAGCCGGAGATCT
  ATTTTTTATATTTCAAGTACACTGAATTTTTATGTGTGATTCAATGCCTCTGGCTCTACA
  CATATAAATTGTCTTAATGGATGAAATCATATTTGGAATAAAAATTCCAGAATGAAGAAT
  TCAGATTGCTGAATGGAGTTAAACTTTAGTGCTACAGAAAAGAAACTCTATGGTCTTATA
  TTTACAACACTTTAATGGGTTTTTTAAAAATCTGTGGAGGTTGCTGGTACACACCAAATG
  AGTCCAAACTGGAATGAGCAGCTTTAGCAAAGAACTCTTACCCTGGCAAAGCAGCAACAC
  ACATGCTCCGTCTGACAAGGTGGTCAACAACATTCCTCAAAATGGGAGATCTTCTCAGCC
  CTGAGGTTTGAATCTGACTTTAGCCTACCTAACCCAGAAAATCTGAATTGGAATGCACTC
  AGACTGTATAAGGACAGTCCTATTTAGACATGTAATTTGTGTAAATTATTGATGAAAATA
  ATTTACTGTGACTTTATTAGCAGCTGACTTTCAAAGTGGATGCAATTTTTCTTTCTTTTG
  TTGGGGAGGGGAATGGGAGGGGAAATGGGAATATAATATTGTCTCTTTTTTAAGTTTGGC
  AAACAGAATGTTCATACTGATGTGTTGTGCCTTAAAGACAAGACAGCATTTGTGTGTTAC
  AATGTAACTTTGGTTAAAATCTCTGTAGATAATGAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004100
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_004100.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004100.2, NP_004091.2
• RefSeq Size: 3077 bp
• RefSeq ORF: 1920 bp
• Locus ID: 2070
• UniProt ID: O95677
• Cytogenetics: 6q23.2
• Domains: Hydrolase
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Gene Summary: This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) encodes isoform a, which is more abundant in adult brain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.