NMDAR1 (GRIN1) (NM_000832) Human Untagged Clone

CAT#: SC308819

GRIN1 (untagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 1 (GRIN1), transcript variant NR1-1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NMDAR1
• Synonyms: GluN1; MRD8; NDHMSD; NDHMSR; NMD-R1; NMDA1; NMDAR1; NR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000832 edited
  AGGACGGCCCGGAAGCCCCGCGGGGGATGCGCCGAGGGCCCCGCGTTCGCGCCGCGCAGA
  GCCAGGCCCGCGGCCCGAGCCCATGAGCACCATGCGCCTGCTGACGCTCGCCCTGCTGTT
  CTCCTGCTCCGTCGCCCGTGCCGCGTGCGACCCCAAGATCGTCAACATTGGCGCGGTGCT
  GAGCACGCGGAAGCACGAGCAGATGTTCCGCGAGGCCGTGAACCAGGCCAACAAGCGGCA
  CGGCTCCTGGAAGATTCAGCTCAATGCCACCTCCGTCACGCACAAGCCCAACGCCATCCA
  GATGGCTCTGTCGGTGTGCGAGGACCTCATCTCCAGCCAGGTCTACGCCATCCTAGTTAG
  CCATCCACCTACCCCCAACGACCACTTCACTCCCACCCCTGTCTCCTACACAGCCGGCTT
  CTACCGCATACCCGTGCTGGGGCTGACCACCCGCATGTCCATCTACTCGGACAAGAGCAT
  CCACCTGAGCTTCCTGCGCACCGTGCCGCCCTACTCCCACCAGTCCAGCGTGTGGTTTGA
  GATGATGCGTGTCTACAGCTGGAACCACATCATCCTGCTGGTCAGCGACGACCACGAGGG
  CCGGGCGGCTCAGAAACGCCTGGAGACGCTGCTGGAGGAGCGTGAGTCCAAGGCAGAGAA
  GGTGCTGCAGTTTGACCCAGGGACCAAGAACGTGACGGCCCTGCTGATGGAGGCGAAAGA
  GCTGGAGGCCCGGGTCATCATCCTTTCTGCCAGCGAGGACGATGCTGCCACTGTATACCG
  CGCAGCCGCGATGCTGAACATGACGGGCTCCGGGTACGTGTGGCTGGTCGGCGAGCGCGA
  GATCTCGGGGAACGCCCTGCGCTACGCCCCAGACGGCATCCTCGGGCTGCAGCTCATCAA
  CGGCAAGAACGAGTCGGCCCACATCAGCGACGCCGTGGGCGTGGTGGCCCAGGCCGTGCA
  CGAGCTCCTCGAGAAGGAGAACATCACCGACCCGCCGCGGGGCTGCGTGGGCAACACCAA
  CATCTGGAAGACCGGGCCGCTCTTCAAGAGAGTGCTGATGTCTTCCAAGTATGCGGATGG
  GGTGACTGGTCGCGTGGAGTTCAATGAGGATGGGGACCGGAAGTTCGCCAACTACAGCAT
  CATGAACCTGCAGAACCGCAAGCTGGTGCAAGTGGGCATCTACAATGGCACCCACGTCAT
  CCCTAATGACAGGAAGATCATCTGGCCAGGCGGAGAGACAGAGAAGCCTCGAGGGTACCA
  GATGTCCACCAGACTGAAGATTGTGACGATCCACCAGGAGCCCTTCGTGTACGTCAAGCC
  CACGCTGAGTGATGGGACATGCAAGGAGGAGTTCACAGTCAACGGCGACCCAGTCAAGAA
  GGTGATCTGCACCGGGCCCAACGACACGTCGCCGGGCAGCCCCCGCCACACGGTGCCTCA
  GTGTTGCTACGGCTTTTGCATCGACCTGCTCATCAAGCTGGCACGGACCATGAACTTCAC
  CTACGAGGTGCACCTGGTGGCAGATGGCAAGTTCGGCACACAGGAGCGGGTGAACAACAG
  CAACAAGAAGGAGTGGAATGGGATGATGGGCGAGCTGCTCAGCGGGCAGGCAGACATGAT
  CGTGGCGCCGCTAACCATAAACAACGAGCGCGCGCAGTACATCGAGTTTTCCAAGCCCTT
  CAAGTACCAGGGCCTGACTATTCTGGTCAAGAAGGAGATTCCCCGGAGCACGCTGGACTC
  GTTCATGCAGCCGTTCCAGAGCACACTGTGGCTGCTGGTGGGGCTGTCGGTGCACGTGGT
  GGCCGTGATGCTGTACCTGCTGGACCGCTTCAGCCCCTTCGGCCGGTTCAAGGTGAACAG
  CGAGGAGGAGGAGGAGGACGCACTGACCCTGTCCTCGGCCATGTGGTTCTCCTGGGGCGT
  CCTGCTCAACTCCGGCATCGGGGAAGGCGCCCCCAGAAGCTTCTCAGCGCGCATCCTGGG
  CATGGTGTGGGCCGGCTTTGCCATGATCATCGTGGCCTCCTACACCGCCAACCTGGCGGC
  CTTCCTGGTGCTGGACCGGCCGGAGGAGCGCATCACGGGCATCAACGACCCTCGGCTGAG
  GAACCCCTCGGACAAGTTTATCTACGCCACGGTGAAGCAGAGCTCCGTGGATATCTACTT
  CCGGCGCCAGGTGGAGCTGAGCACCATGTACCGGCATATGGAGAAGCACAACTACGAGAG
  TGCGGCGGAGGCCATCCAGGCCGTGAGAGACAACAAGCTGCATGCCTTCATCTGGGACTC
  GGCGGTGCTGGAGTTCGAGGCCTCGCAGAAGTGCGACCTGGTGACGACTGGAGAGCTGTT
  TTTCCGCTCGGGCTTCGGCATAGGCATGCGCAAAGACAGCCCCTGGAAGCAGAACGTCTC
  CCTGTCCATCCTCAAGTCCCACGAGAATGGCTTCATGGAAGACCTGGACAAGACGTGGGT
  TCGGTATCAGGAATGTGACTCGCGCAGCAACGCCCCTGCGACCCTTACTTTTGAGAACAT
  GGCCGGGGTCTTCATGCTGGTAGCTGGGGGCATCGTGGCCGGGATCTTCCTGATTTTCAT
  CGAGATTGCCTACAAGCGGCACAAGGATGCTCGCCGGAAGCAGATGCAGCTGGCCTTTGC
  CGCCGTTAACGTGTGGCGGAAGAACCTGCAGCAGTACCATCCCACTGATATCACGGGCCC
  GCTCAACCTCTCAGATCCCTCGGTCAGCACCGTGGTGTGAGGCCCCCGGAGGCGCCCACC
  TGCCCAGTTAGCCCGGCCAAGGACACTGATGGGTCCTGCTGCTCGGGAAGGCCTGAGGGA
  AGCCCACCCGCCCCAGAGACTGCCCACCCTGGGCCTCCCGTCCGTCCGCCCGCCCACCCC
  GCTGCCTGGCGGGCAGCCCCTGCTGGACCAAGGTGCGGACCGGAGCGGCTGAGGACGGGG
  CAGAGCTGAGTCGGCTGGGCAGGGCCGCAGGGCGCTCCGGCAGAGGCAGGGCCCTGGGGT
  CTCTGAGCAGTGGGGAGCGGGGGCTAACTGGCCCCAGGCGGAGGGGCTTGGAGCAGAGAC
  GGCAGCCCCATCCTTCCCGCAGCACCAGCCTGAGCCACAGTGGGGCCCATGGCCCCAGCT
  GGCTGGGTCGCCCCTCCTCGGGCGCCTGCGCTCCTCTGCAGCCTGAGCTCCACCCTCCCC
  TCTTCTTGCGGCACCGCCCACCCACACCCCGTCTGCCCCTTGACCCCACACGCCGGGGCT
  GGCCCTGCCCTCCCCCACGGCCGTCCCTGACTTCCCAGCTGGCAGCGCCTCCCGCCGCCT
  CGGGCCGCCTCCTCCAGACTCGAGAGGGCTGAGCCCCTCCTCTCCTCGTCCGGCCTGCAG
  CCCAGAACGGGCCTCCCCGGGGGTCCCCGGACGCTGGCTCGGGACTGTCTTCAACCCTGC
  CCTGCACCTTGGGCACGGGAGAGCGCCACCCGCCCGCCCCCGCCCTCGCTCCGGGTGCGT
  GACCGGCCCGCCACCTTGTACAGAACCAGCACTCCCAGGGCCCGAGCGCGTGCCTTCCCC
  GTGCGGCCCGTGCGCAGCCGCGCTCTGCCCCTCCGTCCCCAGGGTGCAGGCGCGCACCGC
  CCAACCCCCACCTCCCGGTGTATGCAGTGGTGATGCCTAAAGGAATGTCACGCAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000832 unedited
  NTCAAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGACGGCCCGGAAGCCCCGCGGGGGATGCGCCGAGGGCCCCGCGT
  TCGCGCCGCGCAGAGCCAGGCCCGCGGCCCGAGCCCATGAGCACCATGCGCCTGCTGACG
  CTCGCCCTGCTGTTCTCCTGCTCCGTCGCCCGTGCCGCGTGCGACCCCAAGATCGTCAAC
  ATTGGCGCGGTGCTGAGCACGCGGAAGCACGAGCAGATGTTCCGCGAGGCCGTGAACCAG
  GCCAACAAGCGGCACGGCTCCTGGAAGATTCAGCTCAATGCCACCTCCGTCACGCACAAG
  CCCAACGCCATCCAGATGGCTCTGTCGGTGTGCGAGGACCTCATCTCCAGCCAGGTCTAC
  GCCATCCTAGTTAGCCATCCACCTACCCCCAACGACCACTTCACTCCCACCCCTGTCTCC
  TACACAGCCGGCTTCTACCGCATACCCGTGCTGGGGCTGACCACCCGCATGTCCATCTAC
  TCGGACAAGAGCATCCACCTGAGCTTCCTGCGCACCGTGCCGCCCTACTCCCACCAGTCC
  AGCGTGTGGTTTGAGATGATGCGTGTCTACAGCTGGAACCACATCATCCTGCTGGTCAGC
  GACGACCACGAGGGCCGGGCGGCTCANAAACGCCTGGAGACGCTGCTGGAGGAGCGTGAG
  TCCAAGGCAGAGAAGGTGCTGCAGTTTGACCCCAGGGACCAGAACGTGACGGCCCTGCTG
  ATGGNAGCAAAAGAACTGGAGCCCGGGTCATCATTCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000832 unedited
  AATAGGACGATGGCACTTGCCAGGTCCAGNAAAGCACTGGGGAAGGGTCACAGGGNAGCC
  ACCCGGGNNATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTGGGGGACAATCCTTTAAGCCTCACCACTGGATACCCCCGGAGGGGGGGGTTGGG
  CGGGGCCCCCCCGCCCCCCGGGGACGGGGGGGCAAAACGCGGCTTCCCACGGGCCCAAAG
  GGGAAAGGACCCCCCCGGGCCCCGGGAAGGCTGGTTCTGTAAAAGGGGGGGGGCCCGCCA
  CCCCCCCCGAACAAAGGCGGGGGCCGGCGGGTGGCCCCCTCCCCTGCCCAAAGGGCCGGG
  GAGGGGTGAAAACAATCCCCAACCCACGTTCGGGGACCCCCGGGGGAGCCCCTTCTGGGC
  TTGCGGCCCGAAAAAAAAAAGAAGGGCTCAACCCCTTTCAAATCTGGGAGAAGCCCCCCC
  AAACCGAAGGAAGCGCTCCCCACTGGGAAAACAAGGACCCCCCCGGGGGAAGGCAAGGCC
  CCCCCCCGGCTGGGGGGGGTAAGGGGCCCAACAGGGATTGGGGTGGGCCGGCCCCCCCTG
  AAAAAAGGGAGGGGGGAACCTCAACCTCCAAAAAGACAAAAGGACCCCCAAGGGGGGGAA
  AACCCACCCAGTGGGGCCAGGGACCCCACGGAGACATAAAATAGTCCCACGCAAAAAGAA
  GGGCCCCCCACTATTAATAAAAAAACCATCTCAGGGGGCCAATTAACCCCCCCTGCCCCG
  AGGAAAAAAAACCG
  
• Restriction Sites: Please inquire
• ACCN: NM_000832
• Insert Size: 2658 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000832.5, NP_000823.4
• RefSeq Size: 3902 bp
• RefSeq ORF: 2658 bp
• Locus ID: 2902
• UniProt ID: Q05586
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Calcium signaling pathway, Huntington's disease, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (GluN1-4a, also known as NR1-1) lacks an alternate in-frame exon and uses an alternate splice site in the 3' coding region, compared to variant GluN1-1a. The resulting protein (isoform GluN1-4a, also known as isoform NR1-1) is shorter and has a different C-terminus, compared to isoform GluN1-1a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.