GPR119 (NM_178471) Human Untagged Clone

SKU SC307189

GPR119 (untagged)-Human G protein-coupled receptor 119 (GPR119)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GPR119
• Synonyms: GPCR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_178471, RT-PCR generated; GACATGGAATCATCTTTCTCATTTGGAGTGATCCTTGCTGTCCTGGCCTCCCTCATCATT; GCTACTAACACACTAGTGGCTGTGGCTGTGCTGCTGTTGATCCACAAGAATGATGGTGTC; AGTCTCTGCTTCACCTTGAATCTGGCTGTGGCTGACACCTTGATTGGTGTGGCCATCTCT; GGCCTACTCACAGACCAGCTCTCCAGCCCTTCTCGGCCCACACAGAAGACCCTGTGCAGC; CTGCGGATGGCATTTGTCACTTCCTCCGCAGCTGCCTCTGTCCTCACGGTCATGCTGATC; ACCTTTGACAGGTACCTTGCCATCAAGCAGCCCTTCCGCTACTTGAAGATCATGAGTGGG; TTCGTGGCCGGGGCCTGCATTGCCGGGCTGTGGTTAGTGTCTTACCTCATTGGCTTCCTC; CCACTCGGAATCCCCATGTTCCAGCAGACTGCCTACAAAGGGCAGTGCAGCTTCTTTGCT; GTATTTCACCCTCACTTCGTGCTGACCCTCTCCTGCGTTGGCTTCTTCCCAGCCATGCTC; CTCTTTGTCTTCTTCTACTGCGACATGCTCAAGATTGCCTCCATGCACAGCCAGCAGATT; CGAAAGATGGAACATGCAGGAGCCATGGCTGGAGGTTATCGATCCCCACGGACTCCCAGC; GACTTCAAAGCTCTCCGTACTGTGTCTGTTCTCATTGGGAGCTTTGCTCTATCCTGGACC; CCCTTCCTTATCACTGGCATTGTGCAGGTGGCCTGCCAGGAGTGTCACCTCTACCTAGTG; CTGGAACGGTACCTGTGGCTGCTCGGCGTGGGCAACTCCCTGCTCAACCCACTCATCTAT; GCCTATTGGCAGAAGGAGGTGCGACTGCAGCTCTACCACATGGCCCTAGGAGTGAAGAAG; GTGCTCACCTCATTCCTCCTCTTTCTCTCGGCCAGGAATTGTGGCCCAGAGAGGCCCAGG; GAAAGTTCCTGTCACATCGTCACTATCTCCAGCTCAGAGTTTGATGGCTAAGACGGTA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178471 unedited; NCCCTTNNNNNNNNCTCNNGGGNNNNNNNNTTTNTTCCNCCAGTCAGATTGNTAAGATCA; TAAGGCGCCCGAATCATATGGTCCGACTCGGTCCCTATAAGGCCGCCATGTGCTGGNATT; CGGCTTGNCATGGAATCATCTTTCTCATTTGNGATGTTTCCTTGCTGTCCTGGCCTCCCT; CATCATTGCTACTAACACACTAGTGGCTGTGGCTGTGCTGCTGTTGATCCACAANAATGA; TGGTGTCAGTCTCTGCTTCACCTTGAATCTGGCTGTGGCTGACACCTTGATTGGTGTGGC; CATCTCTGGCCTACTCACAGACCAGCTCCTCCAGCCCTTCTCTGGCCCACAACGGAAGAC; CCTGTGCAACCCTGCGGATGGGAATTTTGTCAATTCCCTACCGCAGCCTGCCTTCTGTCC; CTCACGGGGCATGGCCGGTTCACCCTTTTTGAACAGGGACCCCTTTGCCAATTCAGAGAA; GGCCCTTATGAACGAGGGGAAGAAAATAGACAGGAAACAAAATTTTATAACAATGGGCGC; CGTGGGGCAGGGAGAAGACAAAACACAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAAAA; AGAAAAAAGACAGGAAGAAGTTTTTAAAGAATGAGAGAG;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_178471 unedited; AAGTGAAGGGAGTGCGACTGCGCTCTCCATGGCCCTAGNATGAAGAAGTGCTCACCTCAT; TCCTCCTCTTTCTCTCGGCCAGGAATTGTGGCCCAGAGAGGCCCAGGGAAAGTTCCTGTC; ACATCGTCACTATCTCCAGCTCAGAGTTTGATGGCTAAGACGGTAAAGCCGAATTCTGCA; GATATCCATCACACTGGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCA; TCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCAC; CAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAA; TATTATGGGGTGGAGGGNGGGTGGTATGGAGCAAGGGGCAAGNTGGGAAGACAANCNGNA; GGGNNNGNGGGGNCAANGGGGAACCAAGCNGGAGGGCAGNGGCACAANCNGGGCNCACGG; CAANCNCCGCCCCCGGGGNNCAAGCGAANCNCCGGCCNCAGCCNCCCGAGNNGGNGGGAN; NCCAGGCAGGCAGGACCAGGCCCAGCNAAANNNGGGNNNNNNGGNAGAGACGGGGNGNCA; CCAAAGGGGCCAGGCGGGGCCCCCACGCCCAAACGCAAGGGAACCACCCACCGGGGCCCC; CCAAACGCGGGGAANACAGGCGGGGAACCACGGCGCCCCNGCCAGGCCCGCCGGAANGGA; AAAAAAACAAAACCAGCAGGGAGGGAAGGCCCGGACACCGCAAAAAGGAAGCCGGGGCAA; GGCACCAACCGGGGGGAACAGCGAGAGAGCGGCCAGCGGGGGCCCAAGGCCGACACAAGC; GGGAGGGGGCCACACCAGCAGAAGCCCCGCAAGAAAAAGGAAACCCCGGCCCGCCCAGGG; NAGGGAAAGAGGGGGG;
• Restriction Sites: Please inquire
• ACCN: NM_178471
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178471.1, NP_848566.1
• RefSeq Size: 1008 bp
• RefSeq ORF: 1008 bp
• Locus ID: 139760
• UniProt ID: Q8TDV5
• Cytogenetics: Xq26.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Summary: This gene encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.[provided by RefSeq, Jan 2010]