NT5C1B (NM_033253) Human Untagged Clone

CAT#: SC305600

NT5C1B (untagged)-Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NT5C1B
• Synonyms: AIRP; CN-IB; CN1B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033253 edited
  CAGGTAAAAAAATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGT
  CCTCAAAAGAGAGTCTAGAAGCAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTC
  TGAGCAATCAGGGATCACAAGAATCATCACTGCGGAAGACAGACTCTCGAGGGTACCTTG
  TGCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACCAAGGCTCCATCCATAGATG
  AGCCTAGAAGCAGGAACACCAGTGCTAAGCTCCCCAGCAGCTCCACGAGCTCCCGGACTC
  CATCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGC
  TCCAGCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCC
  CAGAGCCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGG
  CCCAAGGCATCGTGCGGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGC
  GCACGTCCCCCACCGAGTGGAAGTCCTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCA
  CCCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAGCAGCGGGAGGACGAGGACG
  ACTACGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACCCGAGCTGCT
  CGCGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGC
  TCTTCAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGG
  AGTATCAGCTCACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCA
  AGGCACTACAGTATGTCAATGCTAGACTCCGTGATCTATATCCTGATGAACAGGACTTAT
  TTGATATTGTACTGATGACTAATAACCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCG
  TCAATCACTACGGCTTACTGATTGACCGCTTCTGTCTGACCGGGGGAAAAGACCCCATTG
  GCTATTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAAAAGTGCAAG
  AGGCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTT
  ACTGTGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGT
  CTGAACATTTTACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTG
  AAAGTAAGCCTCTTGCTCAGGGTCCCCTAAAAGGCTTTCTGGAAGATTTAGGCAGACTGC
  AAAAGAAGTTCTATGCCAAAAATGAACGGTTACTTTGTCCTATCAGGACCTACCTGGTTA
  CAGCTAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAGACCCTTCGACGCTGGGGTC
  TAGAGATAGACGAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCTTGGTGAAGA
  TCCGGCCCCACATCTTCTTTGATGACCACATGTTCCACATTGAAGGGGCACAGAGGTTAG
  GTTCCATCGCAGCTTATGGCTTTAATAAAAAATTCAGTAGTTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_033253
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: It is not a varient.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033253.2, NP_150278.2
• RefSeq Size: 2684 bp
• RefSeq ORF: 1653 bp
• Locus ID: 93034
• UniProt ID: Q96P26
• Cytogenetics: 2p24.2
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Gene Summary: Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.