NT5C1B (NM_033253) Human Untagged Clone

SKU SC305600

NT5C1B (untagged)-Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: NT5C1B
• Synonyms: AIRP; CN-IB; CN1B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033253 edited; CAGGTAAAAAAATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGT; CCTCAAAAGAGAGTCTAGAAGCAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTC; TGAGCAATCAGGGATCACAAGAATCATCACTGCGGAAGACAGACTCTCGAGGGTACCTTG; TGCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACCAAGGCTCCATCCATAGATG; AGCCTAGAAGCAGGAACACCAGTGCTAAGCTCCCCAGCAGCTCCACGAGCTCCCGGACTC; CATCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGC; TCCAGCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCC; CAGAGCCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGG; CCCAAGGCATCGTGCGGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGC; GCACGTCCCCCACCGAGTGGAAGTCCTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCA; CCCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAGCAGCGGGAGGACGAGGACG; ACTACGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACCCGAGCTGCT; CGCGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGC; TCTTCAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGG; AGTATCAGCTCACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCA; AGGCACTACAGTATGTCAATGCTAGACTCCGTGATCTATATCCTGATGAACAGGACTTAT; TTGATATTGTACTGATGACTAATAACCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCG; TCAATCACTACGGCTTACTGATTGACCGCTTCTGTCTGACCGGGGGAAAAGACCCCATTG; GCTATTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAAAAGTGCAAG; AGGCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTT; ACTGTGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGT; CTGAACATTTTACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTG; AAAGTAAGCCTCTTGCTCAGGGTCCCCTAAAAGGCTTTCTGGAAGATTTAGGCAGACTGC; AAAAGAAGTTCTATGCCAAAAATGAACGGTTACTTTGTCCTATCAGGACCTACCTGGTTA; CAGCTAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAGACCCTTCGACGCTGGGGTC; TAGAGATAGACGAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCTTGGTGAAGA; TCCGGCCCCACATCTTCTTTGATGACCACATGTTCCACATTGAAGGGGCACAGAGGTTAG; GTTCCATCGCAGCTTATGGCTTTAATAAAAAATTCAGTAGTTAG;
• Restriction Sites: Please inquire
• ACCN: NM_033253
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: It is not a varient.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033253.2, NP_150278.2
• RefSeq Size: 2684 bp
• RefSeq ORF: 1653 bp
• Locus ID: 93034
• UniProt ID: Q96P26
• Cytogenetics: 2p24.2
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Summary: Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.