UCP1 (NM_021833) Human Untagged Clone

CAT#: SC304965

UCP1 (untagged)-Human uncoupling protein 1 (mitochondrial, proton carrier) (UCP1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UCP1
• Synonyms: SLC25A7; UCP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021833 edited
  AGAGCAAGGGAAAGGAACTTCCTCCACCTTCGGGGCTGGAGCCCTTTTCCTCTGCATCTC
  CAGTCTCTGAGTGAAGATGGGGGGCCTGACAGCCTCGGACGTACACCCGACCCTGGGGGT
  CCAGCTCTTCTCAGCTGGAATAGCGGCGTGCTTGGCGGACGTGATCACCTTCCCGCTGGA
  CACGGCCAAAGTCCGGCTCCAGGTCCAAGGTGAATGCCCGACGTCCAGTGTTATTAGGTA
  TAAAGGTGTCCTGGGAACAATCACCGCTGTGGTAAAAACAGAAGGGCGGATGAAACTCTA
  CAGCGGGCTGCCTGCGGGGCTTCAGCGGCAAATCAGCTCCGCCTCTCTCAGGATCGGCCT
  CTACGACACGGTCCAGGAGTTCCTCACCGCAGGGAAAGAAACAGCACCTAGTTTAGGAAG
  CAAGATTTTAGCTGGTCTAACGACTGGAGGAGTGGCAGTATTCATTGGGCAACCCACAGA
  GGTCGTGAAAGTCAGACTTCAAGCACAGAGCCATCTCCACGGAATCAAACCTCGCTACAC
  GGGGACTTATAATGCGTACAGAATAATAGCAACAACCGAAGGCTTGACGGGTCTTTGGAA
  AGGGACTACTCCCAATCTGATGAGAAGTGTCATCATCAATTGTACAGAGCTAGTAACATA
  TGATCTAATGAAGGAGGCCTTTGTGAAAAACAACATATTAGCAGATGACGTCCCCTGCCA
  CTTGGTGTCGGCTCTTATCGCTGGATTTTGCGCAACAGCTATGTCCTCCCCGGTGGATGT
  AGTAAAAACCAGATTTATTAATTCTCCACCAGGACAGTACAAAAGTGTGCCCAACTGTGC
  AATGAAAGTGTTCACTAACGAAGGACCAACGGCTTTCTTCAAGGGGTTGGTACCTTCCTT
  CTTGCGACTTGGATCCTGGAACGTCATTATGTTTGTGTGCTTTGAACAACTGAAACGAGA
  ACTGTCAAAGTCAAGGCAGACTATGGACTGTGCCACATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021833 unedited
  NNNGGTTGAGTCAGATTTGTATACGACTCATATAGGCGGCCGCGAATTCCCCTTCCCAGA
  CAGGGAAAGAACTTCCTCCACCTTCGGGGCTGGAGCCCTTTTCCTCTGCATCTCCAGTCT
  CTGAGTGAAGATGGGGGGCCTGACAGCCTCGGACGTACACCCGACCCTGGGGGTCCAGCT
  CTTCTCAGCTGGAATAGCGGCGTGCTTGGCGGACGTGATCACCTTCCCGCTGGACACGGC
  CAAAGTCCGGCTCCAGGTCCAAGGTGAATGCCCGACGTCCAGTGTTATTAGGTATAAAGG
  TGTCCTGGGAACAATCACCGCTGTGGTAAAAACAGAAGGGCGGATGAAACTCTACAGCGG
  GCTGCCTGCGGGGCTTCAGCGGCAAATCAGCTCCGCCTCTCTCAGGATCGGCCTCTACGA
  CACGGTCCAGGAGTTCCTCACCGCAGGGAAAGAAACAGCACCTAGTTTAGGAAGCAAGAT
  TTTAGCTGGTCTAACGACTGGAGGAGTGGCAGTATTCATTGGGCAACCCACAGAGGTCGT
  GAAAGTCAGACTTCAAGCACAGAGCCATCTCCACGGAATCAAACCTCGCTACACGGNGAC
  TTATAATGCGTACAGAATAATAGCAACAACCGAAGGCTTGACGGGTCTTTGGAAAGGGAC
  TACTCCCAATCTGATGAGAAGTGTCATCATCAATTGTACAGAGCTAGTAACATATGATCT
  AATGAANNGAGCCTTTGTGAAAACAACATATTAGCAGATGACGTCCCCTGCCACTTGGTG
  TCGGCTCTTATCGCTGGNATTTGCGCAACAGCTATGTCCCTCCCCGTGGATGTAGTAAAA
  ACCCAGATTATTAATCTCN
  
• Restriction Sites: Please inquire
• ACCN: NM_021833
• Insert Size: 1000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021833.3, NP_068605.1
• RefSeq Size: 1047 bp
• RefSeq ORF: 924 bp
• Locus ID: 7350
• UniProt ID: P25874
• Cytogenetics: 4q31.1
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease, PPAR signaling pathway
• Gene Summary: Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008]