Neurogenin3 (NEUROG3) (NM_020999) Human Untagged Clone

CAT#: SC304889

NEUROG3 (untagged)-Human neurogenin 3 (NEUROG3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Neurogenin3
• Synonyms: Atoh5; bHLHa7; Math4B; NGN-3; ngn3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020999 edited
  GCCCTTCTCTCTATTCTTTTGCGCCGGTAGAAAGGATGACGCCTCAACCCTCGGGTGCGC
  CCACTGTCCAAGTGACCCGTGAGACGGAGCGGTCCTTCCCCAGAGCCTCGGAAGACGAAG
  TGACCTGCCCCACGTCCGCCCCGCCCAGCCCCACTCGCACACGGGGGAACTGCGCAGAGG
  CGGAAGAGGGAGGCTGCCGAGGGGCCCCGAGGAAGCTCCGGGCACGGCGCGGGGGACGCA
  GCCGGCCTAAGAGCGAGTTGGCACTGAGCAAGCAGCGACGGAGTCGGCGAAAGAAGGCCA
  ACGACCGCGAGCGCAATCGAATGCACAACCTCAACTCGGCACTGGACGCCCTGCGCGGTG
  TCCTGCCCACCTTCCCAGACGACGCGAAGCTCACCAAGATCGAGACGCTGCGCTTCGCCC
  ACAACTACATCTGGGCGCTGACTCAAACGCTGCGCATAGCGGACCACAGCTTGTACGCGC
  TGGAGCCGCCGGCGCCGCACTGCGGGGAGCTGGGCAGCCCAGGCGGTTCCCCCGGGGACT
  GGGGGTCCCTCTACTCCCCAGTCTCCCAGGCTGGCAGCCTGAGTCCCGCCGCGTCGCTGG
  AGGAGCGACCCGGGCTGCTGGGGGCCACCTCTTCCGCCTGCTTGAGCCCAGGCAGTCTGG
  CTTTCTCAGATTTTCTGTGAAAGGACCTGTCTGTCGCTGGGCTGTGGGTGCTAAGGGTAA
  GG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020999 unedited
  GTCAGGTCGAATATTGTATACGACTCATATAGGCGGCCGCGNAATCGCCCTTCTCTCTAT
  TCTTTTGCGCCGGTAGAAAGATGACGCCTCAACCCTCGGGTGCGCCCACTGTCCAAGTGA
  CCCGTGAGACGGAGCGGTCCTTCCCCAGAGCCTCGGAAGACGAAGTGACCTGCCCCACGT
  TCCGCCCCGCCCAGCCCCACTCGCACACGGGGGAACTGCGCAGAGGCGGAAGAGGGAGGC
  TGCCGAGGGGCCCCGAGGAAGCTCCGGGCACGGCGCGGGGGACGCAGCCGGCCTAAGAGC
  GAGTTGGCACTGAGCAAGCAGCGACGGAGTCGGCGAAAGAAGGCCAACGACCGCGAGCGC
  AATCGAATGCACAACCTCAACTCGGCACTGGACGCCCTGCGCGGTGTCCTGCCCACCTTC
  CCAGACGACGCGAAGCTCACCAAGATCGAGACGCTGCGCTTCGCCCACAACTACATCTGG
  GCGCTGACTCANACGCTGCGCATAGCGGACCACAGCTTGTACGCGCTGGAGCCGCCGGCG
  CCGCACTGCGGGGAGCTGGGCAGCCCANGCGGTTCCCCCGGNGACTGGGGGTCCCTCTAC
  TCCCCAGTCTCCCAGGCTGGCAGCCTGAGTCCCGCCGCGTCGCTGGNAGAGCGACCCGGG
  CTGCTGGGGGCCACCTNCTTCGNCTGCTTGAGCCCCAGCAGTCTGGCTTTCTCAGATTTT
  CTGTGAAAAGACCTGTCTGTCGCTGGGCTGTGGGTGCTAAGGGTAAGGAAGGGCGAATTC
  AGATCTGGTACCGATATCAAGCTTGTCGACTCTAGATTGCGGCCCGCGTCATAGCTGGTT
  CCTGGACAGATCCCCGGGTGGCATCCCTGTGACCCCTCCCAGTGGCTCTTCTGGCCCTGN
  AA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020999 unedited
  CCATGGGGAATGGCACTNTCCAGGNCCAGGNANGAGCACTGGGGNAGGGTCACAGGGATG
  CCACCCGGGNATCTGGTTCAGGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACA
  AGCTTGATATCGGTACCAGATCTGAATTCGCCCTTCCTTACCCTTAGCACCCACAGCCCA
  GCGACAGACAGGTCCTTTCACAGAAAATCTGAGAAAGCCAGACTGCCTGGGCTCAAGCAG
  GCGGAAGAGGTGGCCCCCAGCAGCCCGGGTCGCTCCTCCAGCGACGCGGCGGGACTCAGG
  CTGCCAGCCTGGGAGACTGGGGAGTAGAGGGACCCCCAGTCCCCGGGGGAACCGCCTGGG
  CTGCCCAGCTCCCCGCAGTGCGGCGCCGGCGGCTCCAGCGCGTACAAGCTGTGGTCCGCT
  ATGCGCAGCGTTTGAGTCAGCGCCCAGATGTAGTTGTGGGCGAAGCGCAGCGTCTCGATC
  TTGGTGAGCTTCGCGTCGTCTGGGAAGGTGGGCAGGACACCGCGCAGGGCGTCCAGTGCC
  GAGTTGAGGTTGTGCATTCGATTGCGCTCGCGGTCGTTGGCCTTCTTTCGCCGACTCCGT
  CGCTGCTTGCTCAGTGCCAACTCGCTCTTAAGCCGGCTGCGTCCCCCGCGCCGTGCCCGG
  AGCTTNCTCGGGGCCCCTCGGCAGCCTCCCTCTTTCGCCTCTGCGCAGTTCCCCCGTGTG
  CCAGTGGNGCTGGGCGGNGCGGACGTGNGGCAAGTCACTTCGTCTTCCGAGGCTCTGGGG
  AAGGACGCNTCGTCTCACGGTCACTTGGACAGTGGGCGCACCCGAAGGTTGAGGCGTCAT
  CCTTT
  
• Restriction Sites: Please inquire
• ACCN: NM_020999
• Insert Size: 700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There are 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acid.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020999.2, NP_066279.2
• RefSeq Size: 1167 bp
• RefSeq ORF: 645 bp
• Locus ID: 50674
• UniProt ID: Q9Y4Z2
• Cytogenetics: 10q22.1
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).[provided by RefSeq, May 2010]