TMEM16B (ANO2) (NM_020373) Human Untagged Clone

CAT#: SC304752

ANO2 (untagged)-Human anoctamin 2 (ANO2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMEM16B
• Synonyms: C12orf3; TMEM16B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020373 edited
  GGCACGAGATGCCTGAAGATATACCCCTGCTCCCTGGCTCCCCACGCCGGCTGAGCCCTC
  AGGCAGGGTCCAGAGGGGGCCAGGGCCCCAAACATGGACAGCAGTGTCTCAAGATGCCAG
  GTCCCCGGGCCCCAGGTCTGCAGGGCGGTTCCAACAGAGATCCTGGCCAGCCCTGCGGTG
  GAGAGAGCACCCGCAGCAGCTCTGTCATCAACAACTATCTGGATGCCAATGAGCCTGTGT
  CCTTGGAGGCCCGTCTTAGCCGCATGCACTTCCATGACAGTCAGAGGAAGGTCGACTACG
  TACTTGCCTACCACTACCGGAAACGCGGGGCGCACCTGGCCCAAGGCTTCCCTGGCCACT
  CGCTGGCTATCGTCTCCAATGGGGAGACAGGCAAGGAGCCTCATGCTGGGGGCCCAGGTG
  ACATTGAGCTGGGGCCGCTCGATGCCCTGGAGGAGGAGAGGAAGGAGCAGCGGGAGGAAT
  TTGAGCACAATCTGATGGAGGCTGGACTGGAGCTTGAGAAGGACTTGGAGAATAAAAGCC
  AGGGATCCATCTTTGTCCGGATACACGCCCCGTGGCAGGTGCTGGCCAGAGAGGCAGAAT
  TCTTGAAGATCAAAGTTCCTACCAAGAAAATGTACGAGATCAAAGCAGGAGGCAGCATTG
  CAAAGAAGTTCAGCGCGGCTCTGCAGAAGCTGAGCTCGCACCTGCAGCCCCGAGTTCCAG
  AACACAGCAACAACAAGATGAAAAACCTCTCCTACCCATTCTCCAGGGAGAAGATGTACC
  TGTACAACATCCAGGAAAAGGACACCTTCTTTGATAATGCCACCCGCAGCCGCATTGTGC
  ACGAGATCCTGAAGCGCACAGCCTGCTCCCGAGCCAACAACACGATGGGTATTAACTCTC
  TGATCGCAAACAATATCTATGAGGCTGCCTACCCTCTTCATGACGGTGAATACGATAGTC
  CAGAGGACGATATGAATGACAGGAAGCTGCTATATCAAGAATGGGCGCGCTATGGAGTGT
  TCTATAAGTTCCAACCTATTGACCTCATCAGAAAGTATTTTGGAGAAAAAATTGGACTGT
  ATTTTGCCTGGCTGGGATTATATACATCATTCCTCATCCCATCTTCTGTAATTGGAGTGA
  TTGTGTTTCTTTATGGATGTGCAACAATTGAAGAAGATATTCCCAGCAGAGAGATGTGTG
  ACCAGCAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTCCTGTGATTACTGGAACC
  TCAGCTCAGCCTGTGGGACCGCGCAGGCCAGCCACCTGTTTGACAACCCTGCCACCGTCT
  TCTTCTCTATCTTCATGGCTCTGTGGGCTACCATGTTCCTGGAAAACTGGAAGAGGCTAC
  AGATGCGACTGGGCTACTTTTGGGACCTGACTGGCATAGAAGAGGAAGAAGAACATTCCA
  GGCCTGAGTATGAAACCAAAGTTCGAGAGAAAATGCTAAAGGAGAGCAACCAGTCTGCTG
  TCCAGAAATTGGAAACAAACACGACGGAGTGTGGCGATGAGGATGATGAAGATAAACTGA
  CCTGGAAGGATCGTTTCCCAGGTTACCTGATGAACTTTGCCTCCATCTTATTCATGATTG
  CCCTGACATTCTCAATCGTCTTTGGGGTGATAGTGTATCGAATAACAACTGCAGCCGCTC
  TGTCTCTCAATAAGGCTACACGCTCCAATGTCCGGGTGACAGTGACAGCAACAGCAGTCA
  TCATCAACCTCGTGGTCATCCTCATCCTGGACGAGATCTACGGCGCTGTGGCCAAGTGGC
  TCACCAAAATTGAGGTTCCGAAAACAGAACAGACTTTTGAAGAGCGCCTGATCCTCAAAG
  CTTTCTTGCTCAAGTTTGTCAATGCCTACTCCCCCATCTTCTATGTGGCCTTTTTCAAAG
  GGAGGTTTGTGGGCAGGCCTGGAAGCTACGTCTATGTATTCGATGGTTACCGCATGGAAG
  AGTGTGCTCCAGGGGGCTGTCTCATGGAGCTCTGCATTCAGCTCAGCATCATCATGTTGG
  GGAAGCAGTTGATCCAGAACAACATCTTTGAGATTGGAGTCCCGAAGCTAAAGAAACTAT
  TTCGAAAGCTGAAAGATGAGACCGAAGCTGGAGAAACTGACTCTGCCCATTCGAAACATC
  CAGAGCAGTGGGACCTAGACTACAGCTTGGAACCATACACAGGACTGACTCCGGAGTACA
  TGGAAATGATCATCCAGTTTGGTTTTGTCACCCTCTTCGTGGCCTCCTTTCCCCTGGCAC
  CTGTGTTTGCCCTCCTCAACAACGTCATTGAAGTGCGGCTCGATGCAAAGAAGTTTGTTA
  CAGAGCTGAGACGGCCGGATGCTGTAAGAACCAAAGATATCGGAATCTGGTTTGACATTC
  TCTCTGGAATTGGCAAGTTCTCTGTTATCAGCAACGCTTTTGTCATTGCGATCACCTCCG
  ACTTTATCCCCCGCCTGGTGTACCAGTACTCCTACAGTCACAATGGGACTCTGCACGGCT
  TTGTCAACCACACCCTCTCCTTTTTCAACGTCAGCCAGCTGAAGGAGGGGACGCAGCCAG
  AAAACTCACAGTTTGACCAGGAGGTTCAGTTCTGCAGGTTTAAGGATTACCGAGAGCCGC
  CATGGGCCCCGAACCCTTATGAGTTTTCGAAACAGTACTGGTTTATTCTGTCCGCCCGTC
  TGGCTTTTGTCATAATCTTCCAGAACCTCGTGATGTTCCTGAGCGTCCTCGTGGACTGGA
  TGATTCCAGACATCCCCACGGACATCAGCGACCAGATCAAGAAAGAGAAGAGCTTATTAG
  TGGATTTCTTCCTGAAAGAGGAGCATGAGAAGCTCAAGCTGATGGATGAGCCGGCTCTGA
  GGAGCCCAGGAGGTGGGGATCGAAGCAGGAGCCGGGCAGCCAGCTCAGCACCTTCAGGCC
  AAAGCCAGCTGGGCAGCATGATGTCGTCAGGCTCTCAGCACACCAATGTGTGAGCAGTTC
  AGCCTGCAGCAGAGGAGGGCACGGCAGCAGAGACACGCACATGCACGTAAGCATGTTCCT
  ACACCCACATGGCGTGCTGTCTGTCTGCAGGGGCCTGGCTTGATGGGGTCTTGAAGAGGG
  GGCAGTGTGTAAAAAGAAAGAGCCTGACAGAATGAGTGTAGGAAGGAAGAGAAATGATGC
  TACATCTCAGAAACTTCAAGCTTAAGTTTCTTTTAGGTCCCCTTTTAAGCTGCATCCTTT
  TGAGGGATTGGAACCCCACTGGGGTCTCTCCACCCCAGAGTCTCAGGCACCGCGAGCATT
  CCCATGTACCCTGGGGCACCCTCTTGTACTTCACCATCATTTCTGACTTTGACCAGCTGT
  AAGAGAAATGCAGTCAGGAGCACAGCATCCCCTACTTACTATCACTACAAGAAAACTGAG
  TCACTTGCAGTGGGGCTGCCACAGTCTCAGCAGATGCAAATTAAGTTACAAGGCTGAATG
  GTGCAGCCGCTGGACCTGATAGTAAGGTGCAAAGGTCCAAACCTGGGCTTGTCTCCACTT
  CACGTCACAAAAACGTTTGGAGCTAAATGCAGATAACCATCATTTTATGAAAGTTCAGAT
  TTAAAATAATAAAATAAATGGACACATTCTTTATAAAGATGCAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_020373
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP and two deletions of 3bp and 12bp.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020373.1, NP_065106.1
• RefSeq Size: 3717 bp
• RefSeq ORF: 3000 bp
• Locus ID: 57101
• Cytogenetics: 12p13.31
• Protein Families: Transmembrane
• Gene Summary: ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011]