DKK2 (NM_014421) Human Untagged Clone

CAT#: SC304099

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DKK2 (untagged)-Human dickkopf homolog 2 (Xenopus laevis) (DKK2)



  "NM_014421" in other vectors (6)

Reconstitution Protocol

USD 450.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DKK2
Synonyms DKK-2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_014421 edited
CTCCCTCCCTTTGCATTCCCACCCCTCCGGGCTTTGCGTCTTCCTGGGGACCCCCTCGCC
GGGAGATGGCCGCGTTGATGCGGAGCAAGGATTCGTCCTGCTGCCTGCTCCTACTGGCCG
CGGTGCTGATGGTGGAGAGCTCACAGATCGGCAGTTCGCGGGCCAAACTCAACTCCATCA
AGTCCTCTCTGGGCGGGGAGACGCCTGGTCAGGCCGCCAATCGATCTGCGGGCATGTACC
AAGGACTGGCATTCGGCGGCAGTAAGAAGGGCAAAAACCTGGGGCAGGCCTACCCTTGTA
GCAGTGATAAGGAGTGTGAAGTTGGGAGGTATTGCCACAGTCCCCACCAAGGATCATCGG
CCTGCATGGTGTGTCGGAGAAAAAAGAAGCGCTGCCACCGAGATGGCATGTGCTGCCCCA
GTACCCGCTGCAATAATGGCATCTGTATCCCAGTTACTGAAAGCATCTTAACCCCTCACA
TCCCGGCTCTGGATGGTACTCGGCACAGAGATCGAAACCACGGTCATTACTCAAACCATG
ACTTGGGATGGCAGAATCTAGGAAGACCACACACTAAGATGTCACATATAAAAGGGCATG
AAGGAGACCCCTGCCTACGATCATCAGACTGCATTGAAGGGTTTTGCTGTGCTCGTCATT
TCTGGACCAAAATCTGCAAACCAGTGCTCCATCAGGGGGAAGTCTGTACCAAACAACGCA
AGAAGGGTTCTCATGGGCTGGAAATTTTCCAGCGTTGCGACTGTGCGAAGGGCCTGTCTT
GCAAAGTATGGAAAGATGCCACCTACTCCTCCAAAGCCAGACTCCATGTGTGTCAGAAAA
TTTGATCACCATTGAGGAACATCATCAATTGCAGACTGTGAAGTTGTGTATTTAATGCAT
TATAGCATGGTGGAAAATAAGGTTCAGATGCAGAAGAATGG
>OriGene 5' read for NM_014421 unedited
GTGGAGTTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCGCCCTTCTCCCTC
CCTTTGCATTCCCACCCCTCCGGGCTTTGCGTCTTCCTGGGGACCCCCTCGCCGGGAGAT
GGCCGCGTTGATGCGGAGCAAGGATTCGTCCTGCTGCCTGCTCCTACTGGCCGCGGTGCT
GATGGTGGAGAGCTCACAGATCGGCAGTTCGCGGGCCAAACTCAACTCCATCAAGTCCTC
TCTGGGCGGGGAGACGCCTGGTCAGGCCGCCAATCGATCTGCGGGCATGTACCAAGGACT
GGCATTCGGCGGCAGTAAGAAGGGCAAAAACCTGGGGCAGGCCTACCCTTGTAGCAGTGA
TAAGGAGTGTGAAGTTGGGAGGTATTGCCACAGTCCCCACCAAGGATCATCGGCCTGCAT
GGTGTGTCGGAGAAAAAAGAAGCGCTGCCACCGAGATGGCATGTGCTGCCCCAGTACCCG
CTGCAATAATGGCATCTGTATCCCAGTTACTGAAAGCATCTTAACCCCTCACATCCCGGC
TCTGGATGGTACTCGGCACAGAGATCGAAACCACGGTCATTACTCAAACCATGACTTGGG
ATGGCAGAATCTAGGAAGACCACACACTAAGATGTCACATATAAAAGGGCATGAAGGAGA
CCCCTGCCTACGATCATCAGACTGCATTGAAGGGTTTTGCTGTGCTCGTCATTTCTGGAC
CAAAATCTGCAAACCAGTGCTCCATCAGGGGGAAGTCTGTACCAAACAACGCAAGAAGGG
TTCTCATGGGCTGGAAATTTTCCAGCGTTGCGACTGTGCGAAGGGCCTGTCTTGCAAGTA
TGGAAAGATGCCACCTACTCCTCCCAAGCCAGACTCCATGTGTGTCAGAAAATTTG
>OriGene 3' read for NM_014421 unedited
NGGCCCTGGNGATGGCACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGATGCC
ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTT
GATATCGGTACCAGATCTGAATTCGCCCTTCCCATTCTTCTGCATCTGAACCTTATTTTC
CACCATGCTATAATGCATTAAATACACAACTTCACAGTCTGCAATTGATGATGTTCCTCA
ATGGTGATCAAATTTTCTGACACACATGGAGTCTGGCTTTGGAGGAGTAGGTGGCATCTT
TCCATACTTTGCAAGACAGGCCCTTCGCACAGTCGCAACGCTGGAAAATTTCCAGCCCAT
GAGAACCCTTCTTGCGTTGTTTGGTACAGACTTCCCCCTGATGGAGCACTGGTTTGCAGA
TTTTGGTCCAGAAATGACGAGCACAGCAAAACCCTTCAATGCAGTCTGATGATCGTAGGC
AGGGGTCTCCTTCATGCCCTTTTATATGTGACATCTTAGTGTGTGGTCTTCCTAGATTCT
GCCATCCCAAGTCATGGTTTGAGTAATGACCGTGGTTTCGATCTCTGTGCCGAGTACCAT
CCAGAGCCGGGATGTGAGGGGTTAAGATGCTTTCAGTAACTGGGATACAGATGCCATTAT
TGCAGCGGGTACTGGGGCAGCACATGCCATCTCGGTGGCAGCGCTTCTTTTTTCTCCGAC
ACACCATGCAGGCCGATGATCCTTGGTGGGGACTGTGGCAATACCTCCCAACTTCACACT
CCTTATCACTGCTACAAGGGTAGGCCTGCCCCANGTTTTTGCCCTTCTTACTGCCGCCGA
ATGCCAGTCCTTGGTACATGCCCGCAGATCGATTGGCGGCCTGACCAGGCGTCTCCCCGC
CG
Restriction Sites Please inquire     
ACCN NM_014421
Insert Size 950 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014421.2, NP_055236.1
RefSeq Size 3659 bp
RefSeq ORF 780 bp
Locus ID 27123
UniProt ID Q9UBU2
Cytogenetics 4q25
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
Protein Pathways Wnt signaling pathway
Gene Summary This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008]

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