UTF1 (NM_003577) Human Untagged Clone

CAT#: SC303351

UTF1 (untagged)-Human undifferentiated embryonic cell transcription factor 1 (UTF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UTF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003577 edited
  ATGCTGCTCCGGCCCCGCAGGCCGCCCCCGCTCGCGCCCCCCGCGCCGCCCTCGCCCGCC
  AGCCCCGACCCCGAGCCGCGGACACCCGGAGACGCCCCGGGGACCCCGCCCCGGAGGCCC
  GCCTCGCCCAGCGCGCTGGGGGAACTCGGGTTGCCGGTGTCCCCGGGCTCGGCGCAGCGC
  ACGCCCTGGAGCGCCCGGGAGACGGAGCTGCTGCTGGGGACGCTGCTGCAACCGGCCGTG
  TGGCGCGCGCTGCTCCTGGACCGCCGCCAGGCCCTGCCCACCTACCGCCGCGTGTCGGCC
  GCGCTGGCCCAGCAGCAGGTGCGCCGCACCCCCGCGCAGTGCCGCCGCCGCTACAAGTTC
  CTTAAAGACAAGTTTCGCGAGGCGCACGGCCAGCCGCCCGGGCCCTTCGACGAGCAGATC
  CGGAAGCTCATGGGGCTGCTGGGCGACAACGGGCGCAAACGGCCTCGCCGCCGCTCCCCG
  GGGTCCGGGCGCCCCCAGCGCGCCCGCCGCCCGGTCCCCAACGCGCACGCGCCGGCTCCC
  AGCGAACCAGACGCCACCCCGCTGCCCACCGCCCGCGACCGCGACGCGGACCCCACCTGG
  ACGCTCCGCTTCAGCCCGTCCCCACCGAAGTCTGCGGACGCCTCCCCCGCCCCCGGCTCC
  CCGCCAGCTCCCGCCCCGACCGCCCTCGCCACCTGCATCCCCGAGGACCGCGCGCCCGTC
  CGCGGCCCCGGGTCCCCGCCGCCACCCCCGGCCCGCGAAGACCCCGACTCGCCGCCCGGC
  CGCCCCGAGGACTGCGCGCCCCCTCCGGCCGCGCCCCCGTCGCTGAACACCGCCCTGCTG
  CAGACCCTGGGGCACCTGGGCGACATCGCGAACATCCTGGGCCCGCTGCGCGACCAGCTG
  CTGACCTTGAACCAGCACGTGGAGCAGCTGCGCGGCGCCTTCGACCAGACAGTGTCCCTG
  GCCGTGGGCTTCATTCTGGGCAGCGCGGCCGCCGAGCGAGGGGTCCTCAGGGACCCGTGC
  CAGTGAGTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003577 unedited
  GTTTCATTTGTATACGACTCATATAGGCGGCCGCGATTCTGCTGCTCCGGCCCCGCAGGC
  CGCCCCCGCTCGCGCCCCCCGCGCCGCCCTCGCCCGCCAGCCCCGACCCCGAGCCGCGGA
  CACCCGGAGACGCCCCGGGGACCCCGCCCCGGAGGCCCGCCTCGCCCAGCGCGCTGGGGG
  AACTCGGGTTGCCGGTGTCCCCGGGCTCGGCGCAGCGCACGCCCTGGAGCGCCCGGGAGA
  CGGAGCTGCTGCTGGGGACGCTGCTGCAACCGGCCGTGTGGCGCGCGCTGCTCCTGGACC
  GCCGCCAGGCCCTGCCCACCTACCGCCGCGTGTCGGCCGCGCTGGCCCAGCAGCAGGTGC
  GCCGCACCCCCGCGCAGTGCCGCCGCCGCTACAAGTTCCTTAAAGACAAGTTTCGCGAGG
  CGCACGGCCAGCCGCCCGGGCCCTTCGACGAGCAGATCCGGAAGCTCATGGGGCTGCTGG
  GCGACAACGGGCGCAAACGGCCTCGCCGCCGCTCCCCGGGGTCCGGGCGCCCCCAGCGCG
  CCCGCCGCCCGGTCCCCAACGCGCACGCGCCGGCTCCCAGCGAACCAGACGCCACCCCGC
  TGCCCACCGCCCGCGACCGCGACGCGGACCCCACCTGGACGCTCCGCTTCAGCCCGTCCC
  CACCCGAGTCTGCGGACGCCTCCCCCGCCCCCGGTTCCCCGCCAGCTCCCGGCCCGAACG
  CCCTTGCCACCTGGATCCCCGAAGACCGCGCGCCCGTTCGCGGGCCCCGGGTCCCCGGCG
  CCACCCCGGGCCGGGGAAGACCCCGAATTGGCCGCCCGGCCCGCCCCAG
  
• Restriction Sites: Please inquire
• ACCN: NM_003577
• Insert Size: 1000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003577.2, NP_003568.2
• RefSeq Size: 1157 bp
• RefSeq ORF: 1026 bp
• Locus ID: 8433
• UniProt ID: Q5T230
• Cytogenetics: 10q26.3
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Gene Summary: The protein encoded by this gene is a leucine zipper-containing transcriptional coactivator that may link the upstream activator ATF2 with the basal transcription complex. The encoded protein is closely associated with chromatin and is required for the proper differentiation of embryonic carcinoma and embryonic stem cells. Found nearly exclusively in pluripotent cells, this protein can also serve as a transcriptional repressor. [provided by RefSeq, Nov 2015]