Frizzled 7 (FZD7) (NM_003507) Human Untagged Clone

CAT#: SC303322

FZD7 (untagged)-Human frizzled family receptor 7 (FZD7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Frizzled 7
• Synonyms: FzE3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003507 edited
  ACTCGCGGCCGGCGATGCGGGACCCCGGCGCGGCCGCTCCGCTTTCGTCCCTGGGCCTCT
  GTGCCCTGGTGCTGGCGCTGCTGGGCGCACTGTCCGCGGGCGCCGGGGCGCAGCCGTACC
  ACGGAGAGAAGGGCATCTCCGTGCCGGACCACGGCTTCTGCCAGCCCATCTCCATCCCGC
  TGTGCACGGACATCGCCTACAACCAGACCATCCTGCCCAACCTGCTGGGCCACACGAACC
  AAGAGGACGCGGGCCTCGAGGTGCACCAGTTCTACCCGCTGGTGAAGGTGCAGTGTTCTC
  CCGAACTCCGCTTTTTCTTATGCTCCATGTATGCGCCCGTGTGCACCGTGCTCGATCAGG
  CCATCCCGCCGTGTCGTTCTCTGTGCGAGCGCGCCCGCCAGGGCTGCGAGGCGCTCATGA
  ACAAGTTCGGCTTCCAGTGGCCCGAGCGGCTGCGCTGCGAGAACTTCCCGGTGCACGGTG
  CGGGCGAGATCTGCGTGGGCCAGAACACGTCGGACGGCTCCGGGGGCCCAGGCGGCGGCC
  CCACTGCCTACCCTACCGCGCCCTACCTGCCGGACCTGCCCTTCACCGCGCTGCCCCCGG
  GGGCCTCAGATGGCAGGGGGCGTCCCGCCTTCCCCTTCTCATGCCCCCGTCAGCTCAAGG
  TGCCCCCGTACCTGGGCTACCGCTTCCTGGGTGAGCGCGATTGTGGCGCCCCGTGCGAAC
  CGGGCCGTGCCAACGGCCTGATGTACTTTAAGGAGGAGGAGAGGCGCTTCGCCCGCCTCT
  GGGTGGGCGTGTGGTCCGTGCTGTGCTGCGCCTCGACGCTCTTTACCGTTCTCACCTACC
  TGGTGGACATGCGGCGCTTCAGCTACCCAGAGCGGCCCATCATCTTCCTGTCGGGCTGCT
  ACTTCATGGTGGCCGTGGCGCACGTGGCCGGCTTCCTTCTAGAGGACCGCGCCGTGTGCG
  TGGAGCGCTTCTCGGACGATGGCTACCGCACGGTGGCGCAGGGCACCAAGAAGGAGGGCT
  GCACCATCCTCTTCATGGTGCTCTACTTCTTCGGCATGGCCAGCTCCATCTGGTGGGTCA
  TTCTGTCTCTCACTTGGTTCCTGGCGGCCGGCATGAAGTGGGGCCACGAGGCCATCGAGG
  CCAACTCGCAGTACTTCCACCTGGCCGCGTGGGCCGTGCCCGCCGTCAAGACCATCACTA
  TCCTGGCCATGGGCCAGGTAGACGGGGACCTGCTGAGCGGGGTGTGCTACGTTGGCCTCT
  CCAGTGTGGACGCGCTGCGGGGCTTCGTGCTGGCGCCTCTGTTCGTCTACCTCTTCATAG
  GCACGTCCTTCTTGCTGGCCGGCTTCGTGTCCCTCTTCCGTATCCGCACCATCATGAAAC
  ACGACGGCACCAAGACCGAGAAGCTGGAGAAGCTCATGGTGCGCATCGGCGTCTTCAGCG
  TGCTCTACACAGTGCCCGCCACCATCGTCCTGGCCTGCTACTTCTACGAGCAGGCCTTCC
  GCGAGCACTGGGAGCGCACCTGGCTCCTGCAGACGTGCAAGAGCTATGCCGTGCCCTGCC
  CGCCCGGCCACTTCCCGCCCATGAGCCCCGACTTCACCGTCTTCATGATCAAGTACCTGA
  TGACCATGATCGTCGGCATCACCACTGGCTTCTGGATCTGGTCGGGCAAGACCCTGCAGT
  CGTGGCGCCGCTTCTACCACAGACTTAGCCACAGCAGCAAGGGGGAGACTGCGGTATGAG
  CCCCGGCCCCTCCCCACCTTTCCCACCCCAGCCCTCTTGCAAGAGGAGAGGCACGGTAGG
  GAAAAGAACTGCTGGGTGGGGGCCTGTTTCTGTAACTTTCTCCCCCTCTACTGAGAAGTG
  ACCTGGAAGTGAGAAGTTTTTTGCAGATTTGGGGCGAGGGGTGATTTGGAAAAGAAGACC
  TGTGTGGAAAGCGGTTTGGATGAAAAGATTTCAGGCAAAGACTTGCAGGAAGATGATGAT
  AACGGCGATGTGAATCGTCAAAGGTACGGGCCAGCTTGTGCCTAATAGAAGGTTGAGACC
  AGCAGAGACTGCTGTGAGTTTCTCCCGGCTCCGAGGCTGAACGGGGACTGTGAGCGATCC
  CCCTGCTGCAGGGCGAGTGGCCTGTCCAGACCCCTGTGAGGCCCCGGGAAAGGTACAGCC
  CTGTCTGCGGTGGCTGCTTTGTTGGAAAGAGGGAGGGCCTCCTGCGGTGTGCTTGTCAAG
  CAGTGGTCAAACCATAATCTCTTTTCACTGGGGCCAAACTGGAGCCCAGATGGGTTAATT
  TCCAGGGTCAGACATTACGGTCTCTCCTCCCCTGCCCCCTCCCGCCTGTTTTTCCTCCCG
  TACTGCTTTCAGGTCTTGTAAAATAAGCATTTGGAAGTCTTGGGAGGCCTGCCTGCTAGA
  ATCCTAATGTGAGGATGCAAAAGAAATGATGATAACATTTTGAGATAAGGCCAAGGAGAC
  GTGGAGTAGGTATTTTTGCTACTTTTTCATTTTCTGGGGAAGGCAGGAGGCAGAAAGACG
  GGTGTTTTATTTGGTCTAATACCCTGAAAAGAAGTGATGACTTGTTGCTTTTCAAAACAG
  GAATGCATTTTTCCCCTTGTCTTTGTTGTAAGAGACAAAAGAGGAAACAAAAGTGTCTCC
  CTGTGGAAAGGCATAACTGTGACGAAAGCAACTTTTATAGGCAAAGCAGCGCAAATCTGA
  GGTTTCCCGTTGGTTGTTAATTTGGTTGAGATAAACATTCCTTTTTAAGGAAAAGTGAAG
  AGCAGTGTGCTGTCACACACCGTTAAGCCAGAGGTTCTGACTTCGCTAAAGGAAATGTAA
  GAGGTTTTGTTGTCTGTTTTAAATAAATTTAATTCGGAACACATGATCCAACAGACTATG
  TTAAAATATTCAGGGAAATCTCTCCCTTCATTTACTTTTTCTTGCTATAAGCCTATATTT
  AGGTTTCTTTTCTATTTTTTTCTCCCATTTGGATCCTTTGAGGTAAAAAAACATAATGTC
  TTCAGCCTCATAATAAAGGAAAGTTAATTAAAAAAAAAAAGCAAAGAAAAAAAAAAAAAA
  AAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_003507
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003507.1, NP_003498.1
• RefSeq Size: 3851 bp
• RefSeq ORF: 1725 bp
• Locus ID: 8324
• UniProt ID: O75084
• Cytogenetics: 2q33.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008]