LIF (NM_002309) Human Untagged Clone

SKU SC303176

LIF (untagged)-Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LIF
• Synonyms: CDF; DIA; HILDA; MLPLI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002309 edited; ATGAAGGTCTTGGCGGCAGGAGTTGTGCCCCTGCTGTTGGTTCTGCACTGGAAACATGGG; GCGGGGAGCCCCCTCCCCATCACCCCTGTCAACGCCACCTGTGCCATACGCCACCCATGT; CACAACAACCTCATGAACCAGATCAGGAGCCAACTGGCACAGCTCAATGGCAGTGCCAAT; GCCCTCTTTATTCTCTATTACACAGCCCAGGGGGAGCCGTTCCCCAACAACCTGGACAAG; CTATGTGGCCCCAACGTGACGGACTTCCCGCCCTTCCACGCCAACGGCACGGAGAAGGCC; AAGCTGGTGGAGCTGTACCGCATAGTCGTGTACCTTGGCACCTCCCTGGGCAACATCACC; CGGGACCAGAAGATCCTCAACCCCAGTGCCCTCAGCCTCCACAGCAAGCTCAACGCCACC; GCCGACATCCTGCGAGGCCTCCTTAGCAACGTGCTGTGCCGCCTGTGCAGCAAGTACCAC; GTGGGCCATGTGGACGTGACCTACGGCCCTGACACCTCGGGTAAGGATGTCTTCCAGAAG; AAGAAGCTGGGCTGTCAACTCCTGGGGAAGTATAAGCAGATCATCGCCGTGTTGGCCCAG; GCCTTCTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002309 unedited; NGAGTACAAAATTTGTATACGACTCACTATAGGCGGCCGCGNATTCATGAAGTCTTGGCG; GCAGGAGTTGTGCCCCTGCTGTTGGTTCTGCACTGGAAACATGGGGCGGGGAGCCCCCTC; CCCATCACCCCTGTCAACGCCACCTGTGCCATACGCCACCCATGTCACAACAACCTCATG; AACCAGATCAGGAGCCAACTGGCACAGCTCAATGGCAGTGCCAATGCCCTCTTTATTCTC; TATTACACAGCCCAGGGGGAGCCGTTCCCCAACAACCTGGACAAGCTATGTGGCCCCAAC; GTGACGGACTTCCCGCCCTTCCACGCCAACGGCACGGAGAAGGCCAAGCTGGTGGAGCTG; TACCGCATAGTCGTGTACCTTGGCACCTCCCTGGGCAACATCACCCGGGACCAGAAGATC; CTCAACCCCAGTGCCCTCAGCCTCCACAGCAAGCTCAACGCCACCGCCGACATCCTGCGA; GGCCTCCTTAGCAACGTGCTGTGCCGCCTGTGCAGCAAGTACCACGTGGGCCATGTGGAC; GTGACCTACGGCCCTGACACCTCGGGTAAGGATGTCTTCCAGAAGAAGAAGCTGGGCTGT; CAACTCCTGNGGAAGTATAAGCAGATCATCGCCGTGTTGGCCCAGGCCTTCTAGGTCGAC; TCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTG; ACCCCTCCCCAGTGCCTCTCCTGGCCCTGGGAGTTGCCACTCCAGTGCCCACCAGCCTTG; TCCTAATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGG; TGGGGGGGGTGGGGTATGGAACAGGGGGCAAAGTGGGGAAGAAACCTGTAGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002309 unedited; GNANGAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA; TGACCGCGGCCGCAATCTAGAGTCGACCTAGAAGGCCTGGGCCAACACGGCGATGATCTG; CTTATACTTCCCCAGGAGTTGACAGCCCAGCTTCTTCTTCTGGAAGACATCCTTACCCGA; GGTGTCAGGGCCGTAGGTCACGTCCACATGGCCCACGTGGTACTTGCTGCACAGGCGGCA; CAGCACGTTGCTAAGGAGGCCTCGCAGGATGTCGGCGGTGGCGTTGAGCTTGCTGTGGAG; GCTGAGGGCACTGGGGTTGAGGATCTTCTGGTCCCGGGTGATGTTGCCCAGGGAGGTGCC; AAGGTACACGACTATGCGGTACAGCTCCACCAGCTTGGCCTTCTCCGTGCCGTTGGCGTG; GAAGGGCGGGAAGTCCGTCACGTTGGGGCCACATAGCTTGTCCAGGTTGTTGGGGAACGG; CTCCCCCTGGGCTGTGTAATAGAGAATAAAGAGGGCATTGGCACTGCCATTGAGCTGTGC; CAGTTGGCTCCTGATCTGGTTCATGAGGTTGTTGTGACATGGGTGGCGTATGGCACAGGT; GGCGTTGACAGGGGTATGGGGAAAAGGGGCCTTCCCGCCCATGTTTCCAGTGCAGAACCA; ACAGCAGGGGCACAACTCCTGCCGCCAAGACCTTCATGAATTCGCGGCCGCCCTATAGTG; AGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCAC; CGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGNGGTTATTACGACATTTTGGAAA; GTCCCGTTGATTT;
• Restriction Sites: Please inquire
• ACCN: NM_002309
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002309.2, NP_002300.1
• RefSeq Size: 3868 bp
• RefSeq ORF: 609 bp
• Locus ID: 3976
• UniProt ID: P15018
• Cytogenetics: 22q12.2
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Summary: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.