IHH (NM_002181) Human Untagged Clone

SKU SC303139

IHH (untagged)-Human Indian hedgehog (IHH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: IHH
• Synonyms: BDA1; HHG2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002181 edited; ATGTCTCCCGCCCGGCTCCGGCCCCGACTGCACTTCTGCCTGGTCCTGTTGCTGCTGCTG; GTGGTGCCGGCGGCATGGGGCTGCGGGCCGGGTCGGGTGGTGGGCAGCCGCCGGCGACCG; CCACGCAAACTCGTGCCGCTCGCCTACAAGCAGTTCAGCCCCAATGTGCCCGAGAAGACC; CTGGGCGCCAGCGGACGCTATGAAGGCAAGATCGCTCGCAGCTCCGAGCGCTTCAAGGAG; CTCACCCCCAATTACAATCCAGACATCATCTTCAAGGACGAGGAGAACACAGGCGCCGAC; CGCCTCATGACCCAGCGCTGCAAGGACCGCCTGAACTCGCTGGCTATCTCGGTGATGAAC; CAGTGGCCCGGTGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGACGGCCACCACTCA; GAGGAGTCCCTGCATTATGAGGGCCGCGCGGTGGACATCACCACATCAGACCGCGACCGC; AATAAGTATGGACTGCTGGCGCGCTTGGCAGTGGAGGCCGGCTTTGACTGGGTGTATTAC; GAGTCAAAGGCCCACGTGCATTGCTCCGTCAAGTCCGAGCACTCGGCCGCAGCCAAGACA; GGCGGCTGCTTCCCTGCCGGAGCCCAGGTACGCCTGGAGAGTGGGGCGCGTGTGGCCTTG; TCAGCCGTGAGGCCGGGAGACCGTGTGCTGGCCATGGGGGAGGATGGGAGCCCCACCTTC; AGCGATGTGCTCATTTTCCTGGACCGCGAGCCTCACAGGCTGAGAGCCTTCCAGGTCATC; GAGACTCAGGACCCCCCACGCCGCCTGGCACTCACACCCGCTCACCTGCTCTTTACGGCT; GACAATCACACGGAGCCGGCAGCCCGCTTCCGGGCCACATTTGCCAGCCACGTGCAGCCT; GGCCAGTACGTGCTGGTGGCTGGGGTGCCAGGCCTGCAGCCTGCCCGCGTGGCAGCTGTC; TCTACACACGTGGCCCTCGGGGCCTACGCCCCGCTCACAAAGCATGGGACACTGGTGGTG; GAGGATGTGGTGGCATCCTGCTTCGCGGCCGTGGCTGACCACCACCTGGCTCAGTTGGCC; TTCTGGCCCCTGAGACTCTTTCACAGCTTGGCATGGGGCAGCTGGACCCCGGGGGAGGGT; GTGCATTGGTACCCCCAGCTGCTCTACCGCCTGGGGCGTCTCCTGCTAGAAGAGGGCAGC; TTCCACCCACTGGGCATGTCCGGGGCAGGGAGCTGAAAGGACTCCACCGCTGCCCTCCTG; GAACTGCTGTACTGGGTCCAGAAGCCTCTCAGCCAGGAGGGAGCTGGCCCTGGAAGGGAC; CTGAGCTGGGGGACACTGGCTCCTGCCATCTCCTCTGCCATGAAGATACACCATTGAGAC; TTGACTGGGCAACACCAGCGTCCCCCACCCCCGTCGTGGTGTAGTCATAGAGCTGCAAGC; TGAGCTGGCGAGGGGATGGTTGTTGACCCCTCTCTCCTAGAGACCTTGAGGCTGGCACGG; CGACTCCCAACTCAGCCTGCTCTCACTACGAGTTTTCATACTCTGCCTCCCCCATTGGGG; AGGGCCCATTCCATCCATCTTAGGCCCCTTTGGGTGGGCTTGCGCCTCAGTTGATGCTGC; TAAATTCCCTGGGAGCCAGCATGGATCTGGCTGGACCCGATGCTGTCCAGAACTGGGAAG; GCCACAGGGGTGGGGCAGCCATCCCGGCCATTCTGAGGTATGACATTCCTCCCCGGCCAC; ACTCCTCAAGACACATCCAGAGACTGTTGCTGTCTGTGGGCAGAGTTCTGTGTTCTGGCC; AATGTGACCGTAGTGCCGGGGACTGGGGGAGGTGGGTTGGATGTGCTTGCCACCCCCCCG; GCTAAGCTCCCCCTTCTGCTGAACCATGATCCCCACCCCCTCCGCCGGTCAGTCTCCCAT; ACCTTATTTATTGGAGTGGAGGGGGAAGCCCATGGGAGAATTTTGGGGATGTTTTGGTCT; TTTCTTCCTTTTGTAATAAAAATTATTTAAGTTGTTAGAAAAAAAAAAAAAAAAAAAAAA; A;
• Restriction Sites: Please inquire
• ACCN: NM_002181
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002181.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002181.1, NP_002172.1
• RefSeq Size: 1236 bp
• RefSeq ORF: 1236 bp
• Locus ID: 3549
• UniProt ID: Q14623
• Cytogenetics: 2q35
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Hedgehog signaling pathway
• Summary: This gene encodes a member of the hedgehog family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including an N-terminal fragment that is involved in signaling. Hedgehog family proteins are essential secreted signaling molecules that regulate a variety of developmental processes including growth, patterning and morphogenesis. The protein encoded by this gene specifically plays a role in bone growth and differentiation. Mutations in this gene are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Nov 2015]