Liver Carboxylesterase 1 (CES1) (NM_001025195) Human Untagged Clone

CAT#: SC302361

CES1 (untagged)-Human carboxylesterase 1 (CES1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Liver Carboxylesterase 1
• Synonyms: ACAT; CE-1; CEH; CES2; hCE-1; HMSE; HMSE1; PCE-1; REH; SES1; TGH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001025195 edited
  GTCGCCCTTCCACGATGTGGCTCCGTGCCTTTATCCTGGCCACTCTCTCTGCTTCCGCGG
  CTTGGGCAGGGCATCCGTCCTCGCCACCTGTGGTGGACACCGTGCATGGCAAAGTGCTGG
  GGAAGTTCGTCAGCTTAGAAGGATTTGCACAGCCTGTGGCCATTTTCCTGGGAATCCCTT
  TTGCCAAGCCGCCTCTTGGACCCCTGAGGTTTACTCCACCGCAGCCTGCAGAACCATGGA
  GCTTTGTGAAGAATGCCACCTCGTACCCTCCTATGTGCACCCAAGATCCCAAGGCGGGGC
  AGTTACTCTCAGAGCTATTTACAAACCGAAAGGAGAACATTCCTCTCAAGCTTTCTGAAG
  ACTGTCTTTACCTCAATATTTACACTCCTGCTGACTTGACCAAGAAAAACAGGCTGCCGG
  TGATGGTGTGGATCCACGGAGGGGGGCTGATGGTGGGTGCGGCATCAACCTATGATGGGC
  TGGCCCTTGCTGCCCATGAAAACGTGGTGGTGGTGACCATTCAATATCGCCTGGGCATCT
  GGGGATTCTTCAGCACAGGGGATGAACACAGCCGGGGGAACTGGGGTCACCTGGACCAGG
  TGGCTGCCCTGCGCTGGGTCCAGGACAACATTGCCAGCTTTGGAGGGAACCCAGGCTCTG
  TGACCATCTTTGGAGAGTCAGCGGGAGGAGAAAGTGTCTCTGTTCTTGTTTTGTCTCCAT
  TGGCCAAGAACCTCTTCCACCGGGCCATTTCTGAGAGTGGCGTGGCCCTCACTTCTGTTC
  TGGTGAAGAAAGGTGATGTCAAGCCCTTGGCTGAGCAAATTGCTATCACTGCTGGGTGCA
  AAACCACCACCTCTGCTGTCATGGTTCACTGCCTGCGACAGAAGACGGAAGAGGAGCTCT
  TGGAGACGACATTGAAAATGAAATTCTTATCTCTGGACTTACAGGGAGACCCCAGAGAGA
  GTCAACCCCTTCTGGGCACTGTGATTGATGGGATGCTGCTGCTGAAAACACCTGAAGAGC
  TTCAAGCTGAAAGGAATTTCCACACTGTCCCCTACATGGTCGGAATTAACAAGCAGGAGT
  TTGGCTGGTTGATTCCAATGCAGTTGATGAGCTATCCACTCTCCGAAGGGCAACTGGACC
  AGAAGACAGCCATGTCACTCCTGTGGAAGTCCTATCCCCTTGTTTGCATTGCTAAGGAAC
  TGATTCCAGAAGCCACTGAGAAATACTTAGGAGGAACAGACGACACTGTCAAAAAGAAAG
  ACCTGTTCCTGGACTTGATAGCAGATGTGATGTTTGGTGTCCCATCTGTGATTGTGGCCC
  GGAACCACAGAGATGCTGGAGCACCCACCTACATGTATGAGTTTCAGTACCGTCCAAGCT
  TCTCATCAGACATGAAACCCAAGACGGTGATAGGAGACCACGGGGATGAGCTCTTCTCCG
  TCTTTGGGGCCCCATTTTTAAAAGAGGGTGCCTCAGAAGAGGAGATCAGACTTAGCAAGA
  TGGTGATGAAATTCTGGGCCAACTTTGCTCGCAATGGAAACCCCAATGGGGAAGGGCTGC
  CCCACTGGCCAGAGTACAACCAGAAGGAAGGGTATCTGCAGATTGGTGCCAACACCCAGG
  CGGCCCAGAAGCTGAAGGACAAAGAAGTAGCTTTCTGGACCAACCTCTTTGCCAAGAAGG
  CAGTGGAGAAGCCACCCCAGACAGAACACATAGAGCTGTGAATGAAGATCCAGCCGGCCT
  TGGGAGCCTGGAGGAGCAAAGACTGGGGTCTTTTGCGAAAGGGATTGCAGGTTCAGAAGG
  CATCTTACCATGGCTGGGGAATTGTCTGGTGGTGGGGGGCAGGGGACAGAGGCCATGAAG
  GAGCAAGTTTTGTATTTGTGACCTCAGCTTTGGGAATAAAGGATCTTTTGAAGGCCAAAA
  AAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001025195
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025195.1, NP_001020366.1
• RefSeq Size: 2027 bp
• RefSeq ORF: 1707 bp
• Locus ID: 1066
• UniProt ID: P23141
• Cytogenetics: 16q12.2
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes
• Gene Summary: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).