KCNK2 (NM_001017424) Human Untagged Clone

SKU SC302049

KCNK2 (untagged)-Human potassium channel, subfamily K, member 2 (KCNK2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: KCNK2
• Synonyms: hTREK-1c; hTREK-1e; K2p2.1; TPKC1; TREK; TREK-1; TREK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001017424 edited; ATGATGAACCCACGAGCAAAAAGGGATTTCTACTTGGCGGCACCTGACTTGCTGGATCCT; AAATCTGCCGCTCAGAACTCCAAACCGAGGCTCTCGTTTTCCACGAAACCCACAGTGCTT; GCTTCCCGGGTGGAGAGTGACACGACCATTAATGTTATGAAATGGAAGACGGTCTCCACG; ATATTCCTGGTGGTTGTCCTCTATCTGATCATCGGAGCCACCGTGTTCAAAGCATTGGAG; CAGCCTCATGAGATTTCACAGAGGACCACCATTGTGATCCAGAAGCAAACATTCATATCC; CAACATTCCTGTGTCAATTCGACGGAGCTGGATGAACTCATTCAGCAAATAGTGGCAGCA; ATAAATGCAGGGATTATACCGTTAGGAAACACCTCCAATCAAATCAGTCACTGGGATTTG; GGAAGTTCCTTCTTCTTTGCTGGCACTGTTATTACAACCATAGGATTTGGAAACATCTCA; CCACGCACAGAAGGCGGCAAAATATTCTGTATCATCTATGCCTTACTGGGAATTCCCCTC; TTTGGTTTTCTCTTGGCTGGAGTTGGAGATCAGCTAGGCACCATATTTGGAAAAGGAATT; GCCAAAGTGGAAGATACGTTTATTAAGTGGAATGTTAGTCAGACCAAGATTCGCATCATC; TCAACAATCATATTTATACTATTTGGCTGTGTACTCTTTGTGGCTCTGCCTGCGATCATA; TTCAAACACATAGAAGGCTGGAGTGCCCTGGACGCCATTTATTTTGTGGTTATCACTCTA; ACAACTATTGGATTTGGTGACTACGTTGCAGGTGGATCCGATATTGAATATCTGGACTTC; TATAAGCCTGTCGTGTGGTTCTGGATCCTTGTAGGGCTTGCTTACTTTGCTGCTGTCCTG; AGCATGATTGGAGATTGGCTCCGAGTGATATCTAAAAAGACAAAAGAAGAGGTGGGAGAG; TTCAGAGCACACGCTGCTGAGTGGACAGCCAACGTCACAGCCGAATTCAAAGAAACCAGG; AGGCGACTGAGTGTGGAGATTTATGACAAGTTCCAGCGGGCCACCTCCATCAAGCGGAAG; CTCTCGGCAGAACTGGCTGGAAACCACAATCAGGAGCTGACTCCTTGTAGGAGGACCCTG; TCAGTGAACCACCTGACCAGCGAGAGGGATGTCTTGCCTCCCTTACTGAAGACTGAGAGT; ATCTATCTGAATGGTTTGACGCCACACTGTGCTGGTGAAGAGATTGCTGTGATTGAGAAC; ATCAAATAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001017424 unedited; TCACATATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGATGATGAACC; CACGAGCAAAAAGGATTTCTACTTGGCGGCACCTGACTTGCTGGATCCTAAATCTGCCGC; TCAGAACTCCAAACCGAGGCTCTCGTTTTCCACGAAACCCACAGTGCTTGCTTCCCGGGT; GGAGAGTGACACGACCATTAATGTTATGAAATGGAAGACGGTCTCCACGATATTCCTGGT; GGTTGTCCTCTATCTGATCATCGGAGCCACCGTGTTCAAAGCATTGGAGCAGCCTCATGA; GATTTCACAGAGGACCACCATTGTGATCCAGAAGCAAACATTCATATCCCAACATTCCTG; TGTCAATTCGACGGAGCTGGATGAACTCATTCAGCAAATAGTGGCAGCAATAAATGCAGG; GATTATACCGTTAGGAAACACCTCCAATCAAATCAGTCACTGGGATTTGGGAAGTTCCTT; CTTCTTTGCTGGCACTGTTATTACAACCATAGGATTTGGAAACATCTCACCACGCACAGA; AGGCGGCAAAATATTCTGTATCATCTATGCCTTACTGGGAATTCCCCTCTTTGGTTTTCT; CTTGGCTGGAGTTGGAGATCAGCTAGGCACCATATTTGGAAAAGGAATTGCCAAAGTGGA; AGATACGTTTATTAAGTGGAATGTTAGTCAGACCAAGATTCGCATCATCTCAACAATCAT; ATTTATACTATTTGGNCTGTGTACCTCTTTGTGGCTCTGCCTGCGATCATATTCAAACCA; TAGAAAGCTGNAGTGCCCTGGACGCCATTATTTTTGTGGTATCACTCTAACACCTATTGG; ATTTGGTGACTACGTTGCAGGTGGATCCGATATTGAATATCTGGGACTTCTTAAACCTGT; CCTGGGGGTCCTGGACCCT;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001017424 unedited; GTAGACGCCTCCCAGTGAGATGGATCAAAGGTGTTGGGTTGGAATCGCTAGGCACCATAT; TTGGAAAAGGAATTGCCAAAGTGGAAGATACGTTTATTAAGTGGAATGTTAGTCAGACCA; AGATTCGCATCATCTCAACAATCATATTTATACTATTTGGCTGTGTACTCTTTGTGGCTC; TGCCTGCGATCATATTCAAACACATAGAAGGCTGGAGTGCCCTGGACGCCATTTATTTTG; TGGTTATCACTCTAACAACTATTGGATTTGGTGACTACGTTGCAGGTGGATCCGATATTG; AATATCTGGACTTCTATAAGCCTGTCGTGTGGTTCTGGATCCTTGTAGGGCTTGCTTACT; TTGCTGCTGTCCTGAGCATGATTGGAGATTGGCTCCGAGTGATATCTAAAAAGACAAAAG; AAGAGGTGGGAGAGTTCAGAGCACACGCTGCTGAGTGGACAGCCAACGTCACAGCCGAAT; TCAAAGAAACCAGGAGGCGACTGAGTGTGGAGATTTATGACAAGTTCCAGCGGGCCACCT; CCATCAAGCGGAAGCTCTCGGCAGAACTGGCTGGAAACCACAATCAGGAGCTGACTCCTT; GTAGGAGGACCCTGTCAGTGAACCACCTGACCAGCGAGAGGGATGTCTTGCCTCCCTTAC; TGAAGACTGAGAGTATCTATCTGAATGGTTTGACGCCACACTGTGCTGGTGAAGAGATTG; CTGTGATTGAGAACATCAAATAGCCCTCTCTTTAAATAACCTTAGGCATAGCCATAGGTG; AGGACTTCTCTATGCTCTTTATGACTGTTGCTGGTAGCATTTTTTAAATTGTGCATGAGC; TCAAAGGGGGAACAAAATAGATACACCCATCATGGTCATCTATCATCAGAGATTTGGAAT; TCTGAGCCAGCCCTTTCTTTCTGATGATGCTTGTTGGAACCGGTTCCACTTCCT;
• Restriction Sites: Please inquire
• ACCN: NM_001017424
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001017424.1, NP_001017424.1
• RefSeq Size: 3340 bp
• RefSeq ORF: 1269 bp
• Locus ID: 3776
• UniProt ID: O95069
• Cytogenetics: 1q41
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Summary: This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longest isoform (a).