GPNMB (NM_001005340) Human Untagged Clone

CAT#: SC300913

GPNMB (untagged)-Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPNMB
• Synonyms: HGFIN; NMB; PLCA3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001005340 edited
  ATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCCACTTGAT
  GCCGCCAAACGATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACATGAGGGAG
  CACAATCAATTAAATGGCTGGTCTTCTGATGAAAATGACTGGAATGAAAAACTCTACCCA
  GTGTGGAAGCGGGGAGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGTGCAGGCG
  GTCCTGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGTGAACCTG
  ATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGAGAAGAACTGC
  AGAAATGAGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATGGTCAGAG
  GACAGTGACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAA
  CCTTTTCCTCACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTT
  GGTCAGTATTTCCAGAAATTGGGACGATGTTCAGTGAGAGTTTCTGTGAACACAGCCAAT
  GTGACACTTGGGCCTCAACTCATGGAAGTGACTGTCTACAGAAGACATGGACGGGCATAT
  GTTCCCATCGCACAAGTGAAAGATGTGTACGTGGTAACAGATCAGATTCCTGTGTTTGTG
  ACTATGTTCCAGAAGAACGATCGAAATTCATCCGACGAAACCTTCCTCAAAGATCTCCCC
  ATTATGTTTGATGTCCTGATTCATGATCCTAGCCACTTCCTCAATTATTCTACCATTAAC
  TACAAGTGGAGCTTCGGGGATAATACTGGCCTGTTTGTTTCCACCAATCATACTGTGAAT
  CACACGTATGTGCTCAATGGAACCTTCAGCCTTAACCTCACTGTGAAAGCTGCAGCACCA
  GGACCTTGTCCGCCACCGCCACCACCACCCAGACCTTCAAAACCCACCCCTTCTTTAGCA
  ACTACTCTAAAATCTTATGATTCAAACACCCCAGGACCTGCTGGTGACAACCCCCTGGAG
  CTGAGTAGGATTCCTGATGAAAACTGCCAGATTAACAGATATGGCCACTTTCAAGCCACC
  ATCACAATTGTAGAGGGAATCTTAGAGGTTAACATCATCCAGATGACAGACGTCCTGATG
  CCGGTGCCATGGCCTGAAAGCTCCCTAATAGACTTTGTCGTGACCTGCCAAGGGAGCATT
  CCCACGGAGGTCTGTACCATCATTTCTGACCCCACCTGCGAGATCACCCAGAACACAGTC
  TGCAGCCCTGTGGATGTGGATGAGATGTGTCTGCTGACTGTGAGACGAACCTTCAATGGG
  TCTGGGACGTACTGTGTGAACCTCACCCTGGGGGATGACACAAGCCTGGCTCTCACGAGC
  ACCCTGATTTCTGTTCCTGACAGAGACCCAGCCTCGCCTTTAAGGATGGCAAACAGCGCC
  CTGATCTCCGTTGGCTGCTTGGCCATATTTGTCACTGTGATCTCCCTCTTGGTGTACAAA
  AAACACAAGGAATACAACCCAATAGAAAATAGTCCTGGGAATGTGGTCAGAAGCAAAGGC
  CTGAGTGTCTTTCTCAACCGTGCAAAAGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGAT
  CCGCTACTCAAAAACCAAGAATTTAAAGGAGTTTCTTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001005340 unedited
  NNGGTTCAAATTTTGTAATACGACTCACTATAGNNGGCGGCCGCGNNAATTCGGCACGAG
  GGGTGGNNACGGGCCCAGNAGNAATTCAGAGTTAAACCTTGAGTGCCTGCGTCCGTGAGA
  ATTCAGCATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCC
  ACTTGATGCCGCCAAACGATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACAT
  GAGGGAGCACAATCAATTAAATGGCTGGTCTTCTGATGAAAATGACTGGAATGAAAAACT
  CTACCCAGTGTGGAAGCGGGGAGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGT
  GCAGGCGGTCCTGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGT
  GAACCTGATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGAGAA
  GAACTGCAGAAATGAGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATG
  GTCAGAGGACAGTGACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGA
  TGGGAAACCTTTTCCTCACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCA
  CACACTTGGTCAGTATTTCCAGAAATTGNGACGATGTTCAGTGAGAGTTTCTGTGAACAC
  AGCCAATGTGACACTTGNGCCTCAACTCATGGAAGTGACTGTCTACAGAAGACATGGACG
  GGCATATGTTCCCATCGCACAAGTGAAAGATGTGTACGTGGTAACAGATCAGATTCCTGT
  GTTTGTGACTATGTTCCAGAAAGAACGATCGAATTCATCCGACGAAACCTTNCTCAAGAT
  CTCCNCATTATGNTTTGATGTCCTGATTCATGATCCCTACCACTTNCTNNCATATNCTAC
  CATTACTA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001005340 unedited
  CATTGATTTGTNACTGCGNANCTCCCNCTTGGTGTACAAAAAACCAGGNAAACAACCCAT
  AGAAAATAGTCCTGGGAATGTGGTCAGAAGCAAAGGCCTGAGTGTCTTTCTCAACCGTGC
  AAAAGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGATCCGCTACTCAAAAACCAAGAATT
  TAAAGGAGTTTCTTAAATTTCGACCTTGTTTCTGAAGCTCACTTTTCAGTGCCATTGATG
  TGAGATGTGCTGGAGTGGCTATTAACCTTTTTTTCCTAAAGATTATTGTTAAATAGATAT
  TGTGGTTTGGGGAAGTTGAATTTTTTATAGGTTAAATGTCATTTTAGAGATGGGGAGAGG
  GATTATACTGCAGGCAGCTTCAGCCATGTTGTGAAACTGATAAAAGCAACTTAGCAAGGC
  TTCTTTTCATTATTTTTTATGTTTCACTTATAAAGTCTTAGGTAACTAGTAGGATAGAAA
  CACTGTGTCCCGAGAGTAAGGAGAGAAGCTACTATTGATTAGAGCCTAACCCAGGTTAAC
  TGCAAGAAGAGGCGGGATACTTTCAGCTTTCCATGTAACTGTATGCATAAAGCCAATGTA
  GTCCAGTTTCTAAGATCATGTTCCAAGCTAACTGAATCCCACTTCAATACACACTCATGA
  ACTCCTGATGGAACAATAACAGGCCCAAGCCTGTGGTATGATGTGCACACTTGCTAGACT
  CAGAAANATACTACTCTCAAAATGGGTGGGAGTNATTTGGTGACNACCTACTTTNGCTGN
  CTGAGTGAAGGATGATATTCATATATTCATTTATTCCATGGACNNATTAGTAGTGGCTNT
  TATATACCANGCATGATGCTGAGTGACACTCTTGTGTATATTTCCAATTTTTGTACAGTC
  GCTGCACATATTTGAAATCATATATTAAGACTTTTCAAGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001005340
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005340.1, NP_001005340.1
• RefSeq Size: 2775 bp
• RefSeq ORF: 1719 bp
• Locus ID: 10457
• UniProt ID: Q14956
• Cytogenetics: 7p15.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).