GPNMB (NM_001005340) Human Untagged Clone

SKU SC300913

GPNMB (untagged)-Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GPNMB
• Synonyms: HGFIN; NMB; PLCA3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001005340 edited; ATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCCACTTGAT; GCCGCCAAACGATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACATGAGGGAG; CACAATCAATTAAATGGCTGGTCTTCTGATGAAAATGACTGGAATGAAAAACTCTACCCA; GTGTGGAAGCGGGGAGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGTGCAGGCG; GTCCTGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGTGAACCTG; ATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGAGAAGAACTGC; AGAAATGAGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATGGTCAGAG; GACAGTGACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAA; CCTTTTCCTCACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTT; GGTCAGTATTTCCAGAAATTGGGACGATGTTCAGTGAGAGTTTCTGTGAACACAGCCAAT; GTGACACTTGGGCCTCAACTCATGGAAGTGACTGTCTACAGAAGACATGGACGGGCATAT; GTTCCCATCGCACAAGTGAAAGATGTGTACGTGGTAACAGATCAGATTCCTGTGTTTGTG; ACTATGTTCCAGAAGAACGATCGAAATTCATCCGACGAAACCTTCCTCAAAGATCTCCCC; ATTATGTTTGATGTCCTGATTCATGATCCTAGCCACTTCCTCAATTATTCTACCATTAAC; TACAAGTGGAGCTTCGGGGATAATACTGGCCTGTTTGTTTCCACCAATCATACTGTGAAT; CACACGTATGTGCTCAATGGAACCTTCAGCCTTAACCTCACTGTGAAAGCTGCAGCACCA; GGACCTTGTCCGCCACCGCCACCACCACCCAGACCTTCAAAACCCACCCCTTCTTTAGCA; ACTACTCTAAAATCTTATGATTCAAACACCCCAGGACCTGCTGGTGACAACCCCCTGGAG; CTGAGTAGGATTCCTGATGAAAACTGCCAGATTAACAGATATGGCCACTTTCAAGCCACC; ATCACAATTGTAGAGGGAATCTTAGAGGTTAACATCATCCAGATGACAGACGTCCTGATG; CCGGTGCCATGGCCTGAAAGCTCCCTAATAGACTTTGTCGTGACCTGCCAAGGGAGCATT; CCCACGGAGGTCTGTACCATCATTTCTGACCCCACCTGCGAGATCACCCAGAACACAGTC; TGCAGCCCTGTGGATGTGGATGAGATGTGTCTGCTGACTGTGAGACGAACCTTCAATGGG; TCTGGGACGTACTGTGTGAACCTCACCCTGGGGGATGACACAAGCCTGGCTCTCACGAGC; ACCCTGATTTCTGTTCCTGACAGAGACCCAGCCTCGCCTTTAAGGATGGCAAACAGCGCC; CTGATCTCCGTTGGCTGCTTGGCCATATTTGTCACTGTGATCTCCCTCTTGGTGTACAAA; AAACACAAGGAATACAACCCAATAGAAAATAGTCCTGGGAATGTGGTCAGAAGCAAAGGC; CTGAGTGTCTTTCTCAACCGTGCAAAAGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGAT; CCGCTACTCAAAAACCAAGAATTTAAAGGAGTTTCTTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001005340 unedited; NNGGTTCAAATTTTGTAATACGACTCACTATAGNNGGCGGCCGCGNNAATTCGGCACGAG; GGGTGGNNACGGGCCCAGNAGNAATTCAGAGTTAAACCTTGAGTGCCTGCGTCCGTGAGA; ATTCAGCATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCC; ACTTGATGCCGCCAAACGATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACAT; GAGGGAGCACAATCAATTAAATGGCTGGTCTTCTGATGAAAATGACTGGAATGAAAAACT; CTACCCAGTGTGGAAGCGGGGAGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGT; GCAGGCGGTCCTGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGT; GAACCTGATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGAGAA; GAACTGCAGAAATGAGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATG; GTCAGAGGACAGTGACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGA; TGGGAAACCTTTTCCTCACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCA; CACACTTGGTCAGTATTTCCAGAAATTGNGACGATGTTCAGTGAGAGTTTCTGTGAACAC; AGCCAATGTGACACTTGNGCCTCAACTCATGGAAGTGACTGTCTACAGAAGACATGGACG; GGCATATGTTCCCATCGCACAAGTGAAAGATGTGTACGTGGTAACAGATCAGATTCCTGT; GTTTGTGACTATGTTCCAGAAAGAACGATCGAATTCATCCGACGAAACCTTNCTCAAGAT; CTCCNCATTATGNTTTGATGTCCTGATTCATGATCCCTACCACTTNCTNNCATATNCTAC; CATTACTA;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001005340 unedited; CATTGATTTGTNACTGCGNANCTCCCNCTTGGTGTACAAAAAACCAGGNAAACAACCCAT; AGAAAATAGTCCTGGGAATGTGGTCAGAAGCAAAGGCCTGAGTGTCTTTCTCAACCGTGC; AAAAGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGATCCGCTACTCAAAAACCAAGAATT; TAAAGGAGTTTCTTAAATTTCGACCTTGTTTCTGAAGCTCACTTTTCAGTGCCATTGATG; TGAGATGTGCTGGAGTGGCTATTAACCTTTTTTTCCTAAAGATTATTGTTAAATAGATAT; TGTGGTTTGGGGAAGTTGAATTTTTTATAGGTTAAATGTCATTTTAGAGATGGGGAGAGG; GATTATACTGCAGGCAGCTTCAGCCATGTTGTGAAACTGATAAAAGCAACTTAGCAAGGC; TTCTTTTCATTATTTTTTATGTTTCACTTATAAAGTCTTAGGTAACTAGTAGGATAGAAA; CACTGTGTCCCGAGAGTAAGGAGAGAAGCTACTATTGATTAGAGCCTAACCCAGGTTAAC; TGCAAGAAGAGGCGGGATACTTTCAGCTTTCCATGTAACTGTATGCATAAAGCCAATGTA; GTCCAGTTTCTAAGATCATGTTCCAAGCTAACTGAATCCCACTTCAATACACACTCATGA; ACTCCTGATGGAACAATAACAGGCCCAAGCCTGTGGTATGATGTGCACACTTGCTAGACT; CAGAAANATACTACTCTCAAAATGGGTGGGAGTNATTTGGTGACNACCTACTTTNGCTGN; CTGAGTGAAGGATGATATTCATATATTCATTTATTCCATGGACNNATTAGTAGTGGCTNT; TATATACCANGCATGATGCTGAGTGACACTCTTGTGTATATTTCCAATTTTTGTACAGTC; GCTGCACATATTTGAAATCATATATTAAGACTTTTCAAGAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001005340
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001005340.1, NP_001005340.1
• RefSeq Size: 2775 bp
• RefSeq ORF: 1719 bp
• Locus ID: 10457
• UniProt ID: Q14956
• Cytogenetics: 7p15.3
• Protein Families: Druggable Genome, Transmembrane
• Summary: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).