VEGFB (NM_003377) Human Untagged Clone

CAT#: SC128250

VEGFB (untagged)-Human vascular endothelial growth factor B (VEGFB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VEGFB
• Synonyms: VEGFL; VRF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003377 edited
  GCGATGCGGGCGCCCCCGGCGGGCGGCCCCGGCGGGCACCATGAGCCCTCTGCTCCGCCG
  CCTGCTGCTCGCCGCACTCCTGCAGCTGGCCCCCGCCCAGGCCCCTGTCTCCCAGCCTGA
  TGCCCCTGGCCACCAGAGGAAAGTGGTGTCATGGATAGATGTGTATACTCGCGCTACCTG
  CCAGCCCCGGGAGGTGGTGGTGCCCTTGACTGTGGAGCTCATGGGCACCGTGGCCAAACA
  GCTGGTGCCCAGCTGCGTGACTGTGCAGCGCTGTGGTGGCTGCTGCCCTGACGATGGCCT
  GGAGTGTGTGCCCACTGGGCAGCACCAAGTCCGGATGCAGATCCTCATGATCCGGTACCC
  GAGCAGTCAGCTGGGGGAGATGTCCCTGGAAGAACACAGCCAGTGTGAATGCAGACCTAA
  AAAAAAGGACAGTGCTGTGAAGCCAGACAGGGCTGCCACTCCCCACCACCGTCCCCAGCC
  CCGTTCTGTTCCGGGCTGGGACTCTGCCCCCGGAGCACCCTCCCCAGCTGACATCACCCA
  TCCCACTCCAGCCCCAGGCCCCTCTGCCCACGCTGCACCCAGCACCACCAGCGCCCTGAC
  CCCCGGACCTGCCGCTGCCGCTGCCGACGCCGCAGCTTCCTCCGTTGCCAAGGGCGGGGC
  TTAGAGCTCAACCCAGACACCTGCAGGTGCCGGAAGCTGCGAAGGTGACACATGGCTTTT
  CAGACTCAGCAGGGTGACTTGCCTCAGAGGCTATATCCCAGTGGGGGAACAAAGAGGAGC
  CTGGTAAAAAACAGCCAAGCCCCCAAGACCTCAGCCCAGGCAGAAGCTGCTCTAGGACCT
  GGGCCTCTCAGAGGGCTCTTCTGCCATCCCTTGTCTCCCTGAGGCCATCATCAAACAGGA
  CAGAGTTGGAAGAGGAGACTGGGAGGCAGCAAGAGGGGTCACATACCAGCTCAGGGGAGA
  ATGGAGTACTGTCTCAGTTTCTAACCACTCTGTGCAAGTAAGCATCTTACAACTGGCTCT
  TCCTCCCCTCACTAAGAAGACCCAAACCTCTGCATAATGGGATTTGGGCTTTGGTACAAG
  AACTGTGACCCCCAACCCTGATAAAAGAGATGGAAGGAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003377 unedited
  AAAAAACGTCAGATTTGTAAACGACTTATATAGGCGGCCGCGNAATTCGCACGAGGGCGA
  TGCGGGCGCCCCCGGCGGGCGGCCCCGGCGGGCACCATGAGCCCTCTGCTCCGCCGCCTG
  CTGCTCGCCGCACTCCTGCAGCTGGCCCCCGCCCAGGCCCCTGTCTCCCAGCCTGATGCC
  CCTGGCCACCAGAGGAAAGTGGTGTCATGGATAGATGTGTATACTCGCGCTACCTGCCAG
  CCCCGGGAGGTGGTGGTGCCCTTGACTGTGGAGCTCATGGGCACCGTGGCCAAACAGCTG
  GTGCCCAGCTGCGTGACTGTGCAGCGCTGTGGTGGCTGCTGCCCTGACGATGGCCTGGAG
  TGTGTGCCCACTGGGCAGCACCAAGTCCGGATGCAGATCCTCATGATCCGGTACCCGAGC
  AGTCAGCTGGGGGAGATGTCCCTGGAAGAACACAGCCAGTGTGAATGCAGACCTAAAAAA
  AAGGACAGTGCTGTGAAGCCAGACAGGGCTGCCACTCCCCACCACCGTCCCCAGCCCCGT
  TCTGTTCCGGGCTGGGACTCTGCCCCCGGAGCACCCTCCCCAGCTGACATCACCCATCCC
  ACTCCAGCCCCAGGCCCCTCTGCCCACGCTGCACCCAGCACCACCAGCGCCCTGACCCCC
  GGACCTGCCGCTGCCGCTGCCGACGCCGCAACTTTCTCCGTTGCCAAAGGCGGGGCTTAT
  AGCTCAACCCAGACCCTGCAAGTGCGGAAGCTGCGAAAGGGACACATGCTTTTCAGACTC
  CACAGGGTGACTTGCCTCANAGCCATTTCCACGGGGGAAACAAGAGGAGCCTGGGAAAAA
  AAACCCAGCCCCCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_003377
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003377.3, NP_003368.1
• RefSeq Size: 1172 bp
• RefSeq ORF: 624 bp
• Locus ID: 7423
• UniProt ID: P49765
• Cytogenetics: 11q13.1
• Domains: PDGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma
• Gene Summary: This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (VEGFB-186) encodes the longer isoform (VEGFB-186, also known as VRF186). This isoform lacks the heparin binding domain and is secreted freely from cells. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.