RSPO3 (NM_032784) Human Untagged Clone

SKU SC128235

RSPO3 (untagged)-Human R-spondin 3 homolog (Xenopus laevis) (RSPO3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RSPO3
• Synonyms: CRISTIN1; PWTSR; THSD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_032784 edited; ATGCACTTGCGACTGATTTCTTGGCTTTTTATCATTTTGAACTTTATGGAATACATCGGC; AGCCAAAACGCCTCCCGGGGAAGGCGCCAGCGAAGAATGCATCCTAACGTTAGTCAAGGC; TGCCAAGGAGGCTGTGCAACATGCTCAGATTACAATGGATGTTTGTCATGTAAGCCCAGA; CTATTTTTTGCTCTGGAAAGAATTGGCATGAAGCAGATTGGAGTATGTCTCTCTTCATGT; CCAAGTGGATATTATGGAACTCGATATCCATATATAAATAAGTGTACAAAATGCAAAGCT; GACTGTGATACCTGTTTCAACAAAAATTTCTGCACAAAATGTAAAAGTGGATTTTACTTA; CACCTTGGAAAGTGCCTTGACAATTGCCCAGAAGGGTTGGAAGCCAACAACCATACTATG; GAGTGTGTCAGTATTGTGCACTGTGAGGTCAGTGAATGGAATCCTTGGAGTCCATGCACG; AAGAAGGGAAAAACATGTGGCTTCAAAAGAGGGACTGAAACACGGGTCCGAGAAATAATA; CAGCATCCTTCAGCAAAGGGTAACCTATGTCCCCCAACAAATGAGACAAGAAAGTGTACA; GTGCAAAGGAAGAAGTGTCAGAAGGGAGAACGAGGAAAAAAAGGAAGGGAGAGGAAAAGA; AAAAAACCTAATAAAGGAGAAAGTAAAGAAGCAATACCTGACAGCAAAAGTCTGGAATCC; AGCAAAGAAATCCCAGAGCAACGAGAAAACAAACAGCAGCAGAAGAAGCGAAAAGTCCAA; GATAAACAGAAATCGGTATCAGTCAGCACTGTACACTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032784 unedited; NCGGCCGCGNAATTCTGCACGAGGGGAAAGNGAAGGCAAGCATTACTGGGTTACTATGCA; CTTGCGACTGATTTCTTGGCTTTTTATCATTTTGAACTTTATGGAATACATCGGCAGCCA; AAACGCCTCCCGGGGAAGGCGCCAGCGAAGAATGCATCCTAACGTTAGTCAAGGCTGCCA; AGGAGGCTGTGCAACATGCTCAGATTACAATGGATGTTTGTCATGTAAGCCCAGACTATT; TTTTGCTCTGGAAAGAATTGGCATGAAGCAGATTGGAGTATGTCTCTCTTCATGTCCAAG; TGGATATTATGGAACTCGATATCCATATATAAATAAGTGTACAAAATGCAAAGCTGACTG; TGATACCTGTTTCAACAAAAATTTCTGCACAAAATGTAAAAGTGGATTTTACTTACACCT; TGGAAAGTGCCTTGACAATTGCCCAGAAGGGTTGGAAGCCAACAACCATACTATGGAGTG; TGTCAGTATTGTGCACTGTGAGGTCAGTGAATGGAATCCTTGGAGTCCATGCACGAAGAA; GGGAAAAACATGTGGCTTCAAAAGAGGGACTGAAACACGGGTCCGAGAAATAATACAGCA; TCCTTCAGCAAAGGGTAACCTATGTCCCCCAACAATGAGACACGAAAGTGTACAGTGCAA; AGGAAGAAGTGTCAGAAGGGAGAACGAGGAAAAAAAGAAGGGAGAGGAAAAGATAAAACC; TAATAAAGGAGGAAGTAAAGAAAGCATTCCTGACAGCAAAAGTCTGGGATCCAGCAAAGA; AATCCCAGAGCACGAGAAAACAAACAGCCAGCGAAGAGCGAAAAGTTCCAGATAAAN;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_032784 unedited; CCTNTATAAATAATGTACAAAATGCAAAGCTGACTGTGATACCTGTTTCACAAAAATTCT; GCACAAAATGTAAAAGTGGATTTTACTTACACCTTGGAAAGTGCCTTGACAATTGCCCAG; AAGGGTTGGAAGCCAACAACCATACTATGGAGTGTGTCAGTATTGTGCACTGTGAGGTCA; GTGAATGGAATCCTTGGAGTCCATGCACGAAGAAGGGAAAAACATGTGGCTTCAAAAGAG; GGACTGAAACACGGGTCCGAGAAATAATACAGCATCCTTCAGCAAAGGGTAACCTATGTC; CCCCAACAAATGAGACAAGAAAGTGTACAGTGCANAGGAAGAAGTGTCAGAAGGGAGAAC; GAGGAANAAAAGGAAGGGAGAGGAAAAGAAAAAAACCNTATAAAGGAGAAAGTAAAGAAG; CAATACCTGACAGCAAAAGTCTGGAATCCAGCANAGAAATCCCAGAGCAACGAGAAAACA; AACAGCAGCAGAAGAAGCGAAAAGTCCAAGATAAACAGAAAATCGTATCAGTCAGCACTG; TACACTAGAGGGTTCCATGAGANTATTGTAGACTCATGATGCTGCTATCTCAACCAGATG; CCCAGGACAAGTGCTCTAGCCCTTNAGACCCACAATGGACATGTCAGTTATTGCTCTGTC; TAAACAACATTCCATGAGTGCTATTATCTTAATACAAGCATAGGTAACCACAAGAGCCCA; AAGATCAAGGAGGGATACCTTCCAAAGGGGTGGCTTGGGGCCTTTCCTGCCTTTTAAAAA; AACAGAATTTCTTGTCAATTTATCCAATGGGCTTTTAGATGTAACACCCAAATGTTGCCA; TTTGGAAAAAAAC;
• Restriction Sites: Please inquire
• ACCN: NM_032784
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032784.3, NP_116173.2
• RefSeq Size: 2165 bp
• RefSeq ORF: 819 bp
• Locus ID: 84870
• UniProt ID: Q9BXY4
• Cytogenetics: 6q22.33
• Domains: FU, tsp_1
• Protein Families: Druggable Genome, Secreted Protein
• Summary: This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]