CD32B (FCGR2B) (NM_004001) Human Untagged Clone

CAT#: SC128159

FCGR2B (untagged)-Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD32B
• Synonyms: CD32; CD32B; FCG2; FCGR2; FCGR2C; FcRII-c; IGFR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004001, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAATCCTGTCATTCTTACCTGTCCTTGCCACTGAGAGTGACTGGGCTGACTGCAAGTCCCCCCAGC
  CTTGGGGTCATATGCTTCTGTGGACAGCTGTGCTATTCCTGGCTCCTGTTGCTGGGACACCTGCAGCTCC
  CCCAAAGGCTGTGCTGAAACTCGAGCCCCAGTGGATCAACGTGCTCCAGGAGGACTCTGTGACTCTGACA
  TGCCGGGGGACTCACAGCCCTGAGAGCGACTCCATTCAGTGGTTCCACAATGGGAATCTCATTCCCACCC
  ACACGCAGCCCAGCTACAGGTTCAAGGCCAACAACAATGACAGCGGGGAGTACACGTGCCAGACTGGCCA
  GACCAGCCTCAGCGACCCTGTGCATCTGACTGTGCTTTCTGAGTGGCTGGTGCTCCAGACCCCTCACCTG
  GAGTTCCAGGAGGGAGAAACCATCGTGCTGAGGTGCCACAGCTGGAAGGACAAGCCTCTGGTCAAGGTCA
  CATTCTTCCAGAATGGAAAATCCAAGAAATTTTCCCGTTCGGATCCCAACTTCTCCATCCCACAAGCAAA
  CCACAGTCACAGTGGTGATTACCACTGCACAGGAAACATAGGCTACACGCTGTACTCATCCAAGCCTGTG
  ACCATCACTGTCCAAGCTCCCAGCTCTTCACCGATGGGGATCATTGTGGCTGTGGTCACTGGGATTGCTG
  TAGCGGCCATTGTTGCTGCTGTAGTGGCCTTGATCTACTGCAGGAAAAAGCGGATTTCAGCTCTCCCAGG
  ATACCCTGAGTGCAGGGAAATGGGAGAGACCCTCCCTGAGAAACCAGCCAATCCCACTAATCCTGATGAG
  GCTGACAAAGTTGGGGCTGAGAACACAATCACCTATTCACTTCTCATGCACCCGGATGCTCTGGAAGAGC
  CTGATGACCAGAACCGTATTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004001 unedited
  CAGTCAGATTTGTATACCATCACTATAGGCGGCCGCGATTCCCGGGATCTGCTGTGCTCT
  GGGCGCCAGCTCGCTCCAGGAGTGATGGGAATCCTGTCATTCTTACCTGTCCTTGCCACT
  GAGAGTGACTGGGCTGACTGCAAGTCCCCCCAGCCTTGGGGTCATATGCTTCTGTGGACA
  GCTGTGCTATTCCTGGCTCCTGTTGCTGGGACACCTGCAGCTCCCCCAAGGCTGTGCTGA
  AACTCGAGCCCCAGTGGATCAACGTGCTCCAGGAGGACTCTGTGACTCTGACATGCCGGG
  GGACTCACAGCCCTGAGAGCGACTCCATTCAGTGGTTCCACAATGGGAATCTCATTCCCA
  CCCACACGCAGCCCAGCTACAGGTTCAAGGCCAACAACAATGACAGCGGGGAGTACACGT
  GCCAGACTGGCCAGACCAGCCTCAGCGACCCTGTGCATCTGACTGTGCTTTCTGAGTGGC
  TGGTGCTCCAGACCCCTCACCTGGAGTTCCAGGAGGGAGAAACCATCGTGCTGAGGTGCC
  ACAGCTGGAAAGACAAGCCTCTGGTCAAGGTCACATTCTTCCAGAATGGAAAATCCAAGA
  AATTTTCCCGTTCGGATCCCAACTTCTCCATCCCACAAGCAAACCACAGTCACAGTGGTG
  ATTACCACTGCACAGGTAACATATGCTACACGCTGGACTCATCCAAGCCTGGGACCATCA
  CTGTCCAAGCTCCCCAGCTCTCACCGGTGGGGATCATTGGGGCTGTGGGCACTGGGATTG
  CCTGTAACGCCAATGGTGCCGCCTGAATGGCCCTTGTCCACTGGAGGGAAAAACCGGATT
  TAACTTCTCCAAGATAACCCGGGGTCCGGGACAAGGGAGAAACCCTTCCCGGGGAAACCA
  GCCAATCCCACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004001 unedited
  NNNNNAAAACTTTGGACGCGGNCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTGGGTTTAAAAAGGGTTTA
  TTAAAAAAGGAATATTTGGTCTTTTTTGAAAAATAAAGAAAATAAAAATCCTCCATAATT
  TTTTCCCCCCGAAACAATCACTTTTAAGGGGCGGGGAGGGGGTAGTCTATTTTAACATTT
  CTGAAATCATATAGATTTACCGGGTGGGTCTCACGGAACCGGGAACAAAAGCCTTTCCCA
  AGGGGGTATTATCGGGTAAAAACAGGGGGTTGGTTAAATGGATTGGCTGGGGGGGTGGGG
  AACGGAAAAACCTTGGTGTCAAAAAAAGCTGGAGCCCAAAAGGGGCCGTCGGGCATTTGG
  GGCCCTATTGGCTGGGGGTTTCAGGGCAGGGGGCTTCAGGGCCCAGGGAGATGACTCTGG
  GAAAAACCTTCTTTTTTGGAACTGGAGGATTTCCCTGGCCTCCCCAGGGGGATTTAGGGC
  CGGGAATACCCGATCTTCCCCCTTTGGTTTTCTTCTCAAAACCCAATGGCAGACAAGGGG
  GACTAAATACGGGTTTGGGCATTAGGGTTTTCCAAAGCATCCGGGGGCCTGAAAAGGGAA
  TAGGGGATTGGGGTCTCAGCCCCCACTTTGGCAGCCTCATCAGGATTAAGGGGGATGGCT
  GGGTTCTCAGGGAGGGGGCTCTCCATTTCCCCGGGCTAGGGGGACCCGGGGGAGCTGGAA
  ACCCCCTTTTCCTGGGGAAGAACAAGGGCCCCTACGGGGGAACAAGGGGGCGCTTAAAGG
  AAACCCGGGGGCCACCGGCCCCAAGGGCCCCCCTCGG
  
• Restriction Sites: Please inquire
• ACCN: NM_004001
• Insert Size: 1600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004001.3, NP_003992.3
• RefSeq Size: 1633 bp
• RefSeq ORF: 933 bp
• Locus ID: 2213
• UniProt ID: P31994
• Cytogenetics: 1q23.3
• Domains: ig, IGc2, IG
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Fc gamma R-mediated phagocytosis, Systemic lupus erythematosus
• Gene Summary: The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).