EGR1 (NM_001964) Human Untagged Clone

CAT#: SC128132

EGR1 (untagged)-Human early growth response 1 (EGR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EGR1
• Synonyms: AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001964 edited
  CAGCTCTCCAGCCTGCTCGTCCAGGATGGCCGCGGCCAAGGCCGAGATGCAGCTGATGTC
  CCCGCTGCAGATCTCTGACCCGTTCGGATCCTTTCCTCACTCGCCCACCATGGACAACTA
  CCCTAAGCTGGAGGAGATGATGCTGCTGAGCAACGGGGCTCCCCAGTTCCTCGGCGCCGC
  CGGGGCCCCAGAGGGCAGCGGCAGCAACAGCAGCAGCAGCAGCAGCGGGGGCGGTGGAGG
  CGGCGGGGGCGGCAGCAACAGCAGCAGCAGCAGCAGCACCTTCAACCCTCAGGCGGACAC
  GGGCGAGCAGCCCTACGAGCACCTGACCGCAGAGTCTTTTCCTGACATCTCTCTGAACAA
  CGAGAAGGTGCTGGTGGAGACCAGTTACCCCAGCCAAACCACTCGACTGCCCCCCATCAC
  CTATACTGGCCGCTTTTCCCTGGAGCCTGCACCCAACAGTGGCAACACCTTGTGGCCCGA
  GCCCCTCTTCAGCTTGGTCAGTGGCCTAGTGAGCATGACCAACCCACCGGCCTCCTCGTC
  CTCAGCACCATCTCCAGCGGCCTCCTCCGCCTCCGCCTCCCAGAGCCCACCCCTGAGCTG
  CGCAGTGCCATCCAACGACAGCAGTCCCATTTACTCAGCGGCACCCACCTTCCCCACGCC
  GAACACTGACATTTTCCCTGAGCCACAAAGCCAGGCCTTCCCGGGCTCGGCAGGGACAGC
  GCTCCAGTACCCGCCTCCTGCCTACCCTGCCGCCAAGGGTGGCTTCCAGGTTCCCATGAT
  CCCCGACTACCTGTTTCCACAGCAGCAGGGGGATCTGGGCCTGGGCACCCCAGACCAGAA
  GCCCTTCCAGGGCCTGGAGAGCCGCACCCAGCAGCCTTCGCTAACCCCTCTGTCTACTAT
  TAAGGCCTTTGCCACTCAGTCGGGCTCCCAGGACCTGAAGGCCCTCAATACCAGCTACCA
  GTCCCAGCTCATCAAACCCAGCCGCATGCGCAAGTACCCCAACCGGCCCAGCAAGACGCC
  CCCCCACGAACGCCCTTACGCTTGCCCAGTGGAGTCCTGTGATCGCCGCTTCTCCCGCTC
  CGACGAGCTCACCCGCCACATCCGCATCCACACAGGCCAGAAGCCCTTCCAGTGCCGCAT
  CTGCATGCGCAACTTCAGCCGCAGCGACCACCTCACCACCCACATCCGCACCCACACAGG
  CGAAAAGCCCTTCGCCTGCGACATCTGTGGAAGAAAGTTTGCCAGGAGCGATGAACGCAA
  GAGGCATACCAAGATCCACTTGCGGCAGAAGGACAAGAAAGCAGACAAAAGTGTTGTGGC
  CTCTTCGGCCACCTCCTCTCTCTCTTCCTACCCGTCCCCGGTTGCTACCTCTTACCCGTC
  CCCGGTTACTACCTCTTATCCATCCCCGGCCACCACCTCATACCCATCCCCTGTGCCCAC
  CTCCTTCTCCTCTCCCGGCTCCTCGACCTACCCATCCCCTGTGCACAGTGGCTTCCCCTC
  CCCGTCGGTGGCCACCACGTACTCCTCTGTTCCCCCTGCTTTCCCGGCCCAGGTCAGCAG
  CTTCCCTTCCTCAGCTGTCACCAACTCCTTCAGCGCCTCCACAGGGCTTTCGGACATGAC
  AGCAACCTTTTCTCCCAGGACAATTGAAATTTGCTAAAGGGAAAGGGGAAAGAAAGGGAA
  AAGGGAGAAAAAGAAACACAAGAGACTTAAAGGACAGGAGGAGGAGATGGCCATAGGAGA
  GGAGGGTTCCTCTTAGGTCAGATGGAGGTTCTCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001964 unedited
  AGGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCTTCAGCTCTCCAGC
  CTGCTCGTCCAGATGGCCGCGGCCAAGGCCGAGATGCAGCTGATGTCCCCGCTGCAGATC
  TCTGACCCGTTCGGATCCTTTCCTCACTCGCCCACCATGGACAACTACCCTAAGCTGGAG
  GAGATGATGCTGCTGAGCAACGGGGCTCCCCAGTTCCTCGGCGCCGCCGGGGCCCCAGAG
  GGCAGCGGCAGCAACAGCAGCAGCAGCAGCAGCGGGGGCGGTGGAGGCGGCGGGGGCGGC
  AGCAACAGCAGCAGCAGCAGCAGCACCTTCAACCCTCAGGCGGACACGGGCGAGCAGCCC
  TACGAGCACCTGACCGCAGAGTCTTTTCCTGACATCTCTCTGAACAACGAGAAGGTGCTG
  GTGGAGACCAGTTACCCCAGCCAAACCACTCGACTGCCCCCCATCACCTATACTGGCCGC
  TTTTCCCTGGAGCCTGCACCCAACAGTGGCAACACCTTGTGGCCCGAGCCCCTCTTCAGC
  TTGGTCAGTGGCCTAGTGAGCATGACCAACCCACCGGCCTCCTCGTCCTCAGCACCATCT
  CCAGCGGCCTCCTCCGCCTCCGCCTCCCAGAGCCCACCCCTGAGCTGCGCAGTGCCATCC
  AACGACAGCAGTCCCATTTACTCAGCGGCACCCACCTTCCCCACGCCGAACACTGACATT
  TTCCCTGAGCCACAAAGCCAGGCCTTCCCGGGCTCGGCAGGGACAGCGCTCCAGTACCCG
  CCTCCTGCCTACCCTGCCGCCAAGGGTGGCTTTCAGGTTCCCATGATCCCCGACTACCTG
  TTTCCACAGCAGCAGGNGGATCTGGGCCTGGGCACCCCAGAACAGAAGCCCCTCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001964 unedited
  TATGGGGTGGCACTTCCAGGNCCAGGNANAGCACTGGGGNAGGGTCACAGGGATGCCACC
  CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGAT
  ATCGGTACCAGATCTGAATTCGGCTTGAGAACCTCCATCTGACCTAAGAGGAACCCTCCT
  CTCCTATGGCCATCTCCTCCTCCTGTCCTTTAAGTCTCTTGTGTTTCTTTTTCTCCCTTT
  TCCCTTTCTTTCCCCTTTCCCTTTAGCAAATTTCAATTGTCCTGGGAGAAAAGGTTGCTG
  TCATGTCCGAAAGCCCTGTGGAGGCGCTGAAGGAGTTGGTGACAGCTGAGGAAGGGAAGC
  TGCTGACCTGGGCCGGGAAAGCAGGGGGAACAGAGGAGTACGTGGTGGCCACCGACGGGG
  AGGGGAAGCCACTGTGCACAGGGGATGGGTAGGTCGAGGAGCCGGGAGAGGAGAAGGAGG
  TGGGCACAGGGGATGGGTATGAGGTGGTGGCCGGNGATGGATAAGAGGTAGTAACCGGGG
  ACGGGTAAGAGGTAGCAACCGGGGACGGGTAGGAAGAGAGAGAGGNANGTGGCCGAANAG
  GCCACAACACTTTTGTCTGCTTTCTTGTCCTTCTGCCGCAAGTGGATCTTGGTATGCCTC
  TTGCGTTCATCGCTCCTGGCAAACTTTCTTCCACAGATGTCGCAAGCGAAAGGCTTTTCG
  CCTGTGTGGGTGCCGATGTGGGTGGTGAAGGTGGTCCCTGCCGGCTGAAGTTGCCCATGC
  AAAATGCCGGACTGGAAAGGCCTCCGGGCCTGTGTGGATCCGGATAGTGCCCGGTGAGCT
  TCTCCGAACCGGGAAAAAACGGCCAATCCAGGACTCCACTGGGGCAAGCCGTAAGGCCGT
  TCCTGGGGGGGGCGTTCTGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001964
• Insert Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone is found to be a perfect match to NM_001964.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001964.2, NP_001955.1
• RefSeq Size: 3136 bp
• RefSeq ORF: 1632 bp
• Locus ID: 1958
• UniProt ID: P18146
• Cytogenetics: 5q31.2
• Domains: zf-C2H2
• Protein Families: Druggable Genome
• Protein Pathways: Prion diseases
• Gene Summary: The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014]