COP1 (RFWD2) (NM_022457) Human Untagged Clone

CAT#: SC128099

RFWD2 (untagged)-Human ring finger and WD repeat domain 2 (RFWD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COP1
• Synonyms: CFAP78; FAP78; RFWD2; RNF200
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022457 edited
  CTGCCTTAAAAAAAAACGCAACTTTATTGTTCCTCAGCCCCACCTCCGGCTCGGCGGGCG
  TCCTCAGGATGCACTGAGGCTGAGGGGAGGGGAGGCGGCGGAGGGTCGAGGTCGCGGTCC
  CTCTCCTCCGAGCGCCCGGCTGGAGGGGAGGGAGTCACGATGTCTGGTAGCCGCCAGGCC
  GGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCC
  TCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTTCCGTGGCGGTTTCGGCGGCAGCGCTG
  GTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGCCTCGGGGGCCCGGTGCGGCCT
  GTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCC
  CGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCGTAGGAGGCAGCAGCTCCAGCCTAGGC
  AGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGCAACGGGCTCATCAACTCCTAC
  GAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATAC
  ATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATTCATCAGAGTTTGGAGGACAAT
  AATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATTGACCATCTGTATCCTAATTTC
  TTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTG
  GACCACTCAGTGAGTAGCACCAATGGCCACAGGTGGCAGATATTTCAAGATTGGTTGGGA
  ACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTACTAGTGCAG
  AAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTC
  CTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAACAGATCCAGAAGGAGCTAAGT
  GTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACTCTCCTGTCAGT
  GAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGAT
  TCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCT
  TGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTG
  GAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGAACTGCAAGC
  CAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACTCGATATAATTCAGTACGACCT
  TTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATT
  GAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAGATTAAAGTC
  TATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAATG
  ACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCT
  AGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAG
  GTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGACTTTAATTTGATGGATCCTAAA
  CTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAAC
  TCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGCCCCTCTTCC
  AGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATGATCTTCGTAAC
  ACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTT
  GTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTA
  GGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTTGTAGGC
  CTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTAC
  TATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAAAGTGTTCTC
  GACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTTAGTGCTGTGTGCTGGAGGGCA
  CTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAACAGTCAGGGTACAATTAAGGTG
  CTAGAATTGGTATGAAGGGTTAACTCAAGTCAAATTGTACTTGATCCTGCTGAAATACAT
  CTGCAGCTGACAATGAGAGAAGAAACAGAAAATGTCATGTGATGTCTCTCCCCAAAGTCA
  TCATGGGTTTTGGATTTGTTTTGAATATTTTTTTCTTTTTTTCTTTTCCCTCCTTTATGA
  CCTTTGGGACATTGGGAATACCCAGCCAACTCTCCACCATCAATGTAACTCCATGGACAT
  TGCTGCTCTTGGTGGTGTTATCTAATTTTTGTGATAGGGAAACAAATTCTTTTGAATAAA
  AATAAATAACAAAACAATAAAAGTTTATTGAGCCACAAAAAAAAAAAAAAAAAAAAAAAA
  AAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022457
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The insert of this clone has been fully sequenced and found to be a perfect match to NM_022457.5.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022457.5, NP_071902.2
• RefSeq Size: 2801 bp
• RefSeq ORF: 2196 bp
• Locus ID: 64326
• UniProt ID: Q8NHY2
• Cytogenetics: 1q25.1-q25.2
• Domains: WD40, RING
• Protein Pathways: p53 signaling pathway, Ubiquitin mediated proteolysis
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).