p21 Ras (HRAS) (NM_005343) Human Untagged Clone

CAT#: SC128077

HRAS (untagged)-Human v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p21 Ras
• Synonyms: C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; p21ras; RASH1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005343 edited
  GAATTCGGCACGAGGCGGGGCGGGGCGTGCGCAGGCCCGCCCGAGTCTCCGCCGCCCGTG
  CCCTGCGCCCGCAACCCGAGCCGCACCCGCCGCGGACGGAGCCCATGCGCGGGGCGAACC
  GCGCGCCCCCGCCCCCGCCCCGCCCCGGCCTCGGCCCCGGCCCTGGCCCCGGGGGCAGTC
  GCGCCTGTGAACGGTGGGGCAGGAGACCCTGTAGGAGGACCCCGGGCCGCAGGCCCTTGA
  GGAGCGATGACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCG
  CTGACCATCCAGCTGATCCAGAACCATTTTGTGGACGAATACGACCCCACTATAGAGGAT
  TCCTACCGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGACATCCTGGATACC
  GCCGGCCAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTC
  CTGTGTGTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAG
  CAGATCAAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGTGT
  GACCTGGCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACCTCGTCCGAAGCTACGGC
  ATCCCCTACATCGAGACCTCGGCCAAGACCCGGCAGGGAGTGGAGGATGCCTTCTACACG
  TTGGTGCGTGAGATCCGGCAGCACAAGCTGCGGAAGCTGAACCCTCCTGATGAGAGTGGC
  CCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCCTGACGCAGCACAAGCTCAGGACATGGA
  GGTGCCGGATGCAGGAAGGAGGTGCAGACGGAAGGAGGAGGAAGGAAGGACGGAAGCAAG
  GAAGGAAGGAAGGGCTGCTGGAGCCCAGTCACCCCGGGACCGTGGGCCGAGGTGACTGCA
  GACCCTCCCAGGGAGGCTGTGCACAGACTGTCTTGAACATCCCAAATGCCACCGGAACCC
  CAGCCCTTAGCTCCCCTCCCAGGCCTCTGTGGGCCCTTGTCGGGCACAGATGGGATCACA
  GTAAATTATTGGATGGTCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005343 unedited
  TTGTAATACGACCTCACTATAGGGGGCGGCCGCGATTCGGCACGAGGCGGGGCGGGGCGT
  GCGCAGGCCCGCCCGAGTCTCCGCCGCCCGTGCCCTGCGCCCGCAACCCGAGCCGCACCC
  GCCGCGGACGGAGCCCATGCGCGGGGCGAACCGCGCGCCCCCGCCCCCGCCCCGCCCCGG
  CCTCGGCCCCGGCCCTGGCCCCGGGGGCAGTCGCGCCTGTGAACGGTGGGGCAGGAGACC
  CTGTAGGAGGACCCCGGGCCGCAGGCCCTTGAGGAGCGATGACGGAATATAAGCTGGTGG
  TGGTGGGCGCCGGCGGTGTGGGCAACAGTGCGCTGACCATCCAGCTGATCCAGAACCATT
  TTGTGGACGAATACGACCCCACTATAAAGGATTCCTACCGGAAGCAGGTGGTCATTGATG
  GGGAGACGTGCCTGTGGGACATCCTGGATACCGTCGGCCAGGAGGAGTACAGCGCCCTGC
  GGGACCATTTCATGCCCACCGTGGAGGTCTTGCTGTGCTTGTTTGCCATCAACCTCACCA
  AGTCTTTTGAGGACCTCCTCTCTGACAGGGAGCATTTCAAACTGTTGAAGGACTCGGATG
  ACTTGCCCATGGTGCTGGTGCGGAACCAGTGTGAGCTGGCTCGTCCCACTGCGGATTCCC
  CGGAGGCGCAAGACCTAGTACTATTCTATCGTGTCCCCGTAATGCATCACCTCGCCCTAA
  AACCCCGCATGGTATTGGTAGTTGCCTTCTTCCACCTTGCTCCCTTTTATCTGCCGGCCC
  ACCCACGCTCCGGCCAACTGCACCCCTCTCTTATATAATCGCCCCCCCGCTTCATTCATC
  TGGCATTTTTTGTGCTACCCCTCGTCCCATCCCGAATCGCCCTGCCCTTGCTCGTCCGCC
  TCTCTCTTGATTGTTCGTCTCACCTCAAAAGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005343 unedited
  AACTGGCTTTATAAGGCGGCATCCGCGGTTTNGCCCGACGCCCGGCCCACAGNAGGCCNG
  GGNAGTTTTAGCTAAGGCTGGGGNACCAAAGGCCTTTGGGATGTTCAAGACAGTCTGTGC
  ACAGCCTCCCTGGGAGGGTCTGCAGTCACCTCGGCCCACGGTCCCTGTTTGACTGGGCTC
  CAGCAGCCCTTCCTTCCTTCCTTGCTTCCGTCCTTCCATACATTTCCANCCGTTTGCACC
  TCCTTCCTGCATCCGGCACCTTTTTTTCTGAGCTTGTGCTGCGTCAGGAGAGCACACACT
  TGCAGCTCATGCAGCCGGGGCCACTCTCATCAGGAGGGTTCAGCTTCCGCAGCTTGTGCT
  GCCGGATCTCACGCACCAACGTGTAGAAGGCATCCTCCACTCCCTGCCGGGTCTTGGCCG
  AGGTCTCGATGTAGGGGATGCCGTAGCTTCGGACGAGGTCCTGAGCCTGCCGAGATTCCA
  CAGTGCGTGCAGCCAGGTCACACTTGTTCCCCACCAGCACCATGGGCACGTCATCCGAGT
  CCTTCACCCGTTTGATCTGCTCCCTGTACTGGTGGATGTCCTCAAAAGACTTGGTGTTGG
  TGATGGCAAACACACACAGGAAGCCCTCCCCGGTGCGCATGTACTGGTCCCGCATGGCGC
  TTGACTCCTCCCAGCCCGGCAGTAATCCAGATGTCCAACAGGGACGTTTCCCCCAAAATG
  ACCACTTGTTCCCGTAGGAATCCCTTAAATGGGGTCGGATTCGTCCCAAAAAGGTTCTTG
  GATCAACTGGTGGGCACGGAACTCTGGCCCAACCGGCGGAGCGCACAACACCAGGTTAAA
  TTCCGGTATAGTCCCTAAGGGCCGGGGGCCGGGGGCCCCCTCACAGGGCTCGGGCCCCAC
  CGTTACAAAGCCCACTGGCCCGGGGGCCAGGCCCGGGCCCAGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005343
• Insert Size: 1250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005343.2, NP_005334.1
• RefSeq Size: 1061 bp
• RefSeq ORF: 570 bp
• Locus ID: 3265
• UniProt ID: P01112
• Cytogenetics: 11p15.5
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Endocytosis, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway
• Gene Summary: This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, cognitive disability, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Both variants 1 and 3 encode the same isoform (1).