Calcineurin B (PPP3R1) (NM_000945) Human Untagged Clone

CAT#: SC127998

PPP3R1 (untagged)-Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Calcineurin B
• Synonyms: CALNB1; CNB; CNB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000945 edited
  ATGGGAAATGAGGCAAGTTATCCTTTGGAAATGTGCTCACACTTTGATGCGGATGAAATT
  AAAAGGCTAGGAAAGAGATTTAAGAAGCTTGATTTGGACAATTCTGGTTCTTTGAGTGTG
  GAAGAGTTCATGTCTCTGCCTGAGTTACAACAGAATCCTTTAGTACAGCGAGTAATAGAT
  ATATTCGACACAGATGGGAATGGAGAAGTAGACTTTAAAGAATTCATTGAGGGCGTCTCT
  CAGTTCAGTGTCAAAGGAGATAAGGAGCAGAAATTGAGGTTTGCTTTCCGTATCTATGAC
  ATGGATAAAGATGGCTATATTTCCAATGGGGAACTCTTCCAGGTATTGAAGATGATGGTG
  GGGAACAATCTGAAAGATACACAGTTACAGCAAATTGTAGACAAAACCATAATAAATGCA
  GATAAGGATGGAGATGGAAGAATATCCTTTGAAGAATTCTGTGCTGTTGTAGGTGGCCTA
  GATATCCACAAAAAGATGGTGGTAGATGTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000945 unedited
  GTAGCGTCAAGAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCTCC
  CCGCCGGCGACCCGAGGGCCGCAGCTGGGCCGCCGCCGCCGTTTCCTGCGAGCCAGCCTG
  AGCGCAACACTTCTCCGAGCCAGCGAGCCAGCGAGCCGCCGACCCGCCGAGCAAAATGGG
  AAATGAGGCAAGTTATCCTTTGGAAATGTGCTCACACTTTGATGCGGATGAAATTAAAAG
  GCTAGGAAAGAGATTTAAGAAGCTTGATTTGGACAATTCTGGTTCTTTGAGTGTGGAAGA
  GTTCATGTCTCTGCCTGAGTTACAACAGAATCCTTTAGTACAGCGAGTAATAGATATATT
  CGACACAGATGGGAATGGAGAAGTAGACTTTAAAGAATTCATTGAGGGCGTCTCTCAGTT
  CAGTGTCAAAGGAGATAAGGAGCAGAAATTGAGGTTTGCTTTCCGTATCTATGACATGGA
  TAAAGATGGCTATATTTCCAATGGGGAACTCTTCCAGGTATTGAAGATGATGGTGGGGAA
  CAATCTGAAAGATACACAGTTACAGCAAATTGTAGACAAAACCATAATAAATGCAGATAA
  GGATGGAGATGGAAGAATATCCTTTGAAGAATTCTGTGCTGTTGTAGGTGGCCTAGATAT
  CCACAAAAAGATGGTGGTAGATGTGTGACTCTTATCAGAGAGTACCACCCAACACTTTTG
  CTTTCTTCTCCATCTCTGAAGATCTGCTCAGGACGTCCAGCAATGCTCTCTGTGTATTTA
  AATGGAAGTATTTTTCTCTGTGAACCCCCATTTTCCACATGAGCCTCATGAACCCACTAA
  GTGTTATTGGACTGTAATTCCTCAATAACTCAGTGTAGCACTTTAAAGTCTGGAGGACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000945 unedited
  TTGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTCAAAGTTCAAAATTTTTTATTTTCTTATTCATCCAGGA
  GGAAGTTTTTTAAAAACCCCACAACTTGACGTTTTTTGCAAAAAAAAAACTAAACTTTCA
  ACTTAAACCATCCCTTCCTTTACCCCCCCCCACACAACAATCTTTNAAAAAAAAAAAAAA
  AAGGGAAAAAAGGAAAGGGAAAAACCCCTCTTTCCCCGGAAGGGAAATAAGGTTTACCCT
  TTTTTTTTGTACCCCCCCCCAAAATTTGCCCAACCAAAAAAAATAGAAGGCTTTGGCTAA
  AATTTTGGTAACAAATCCTTTTGTCCAAAAAATTCCCAAAGGGCTAGTCCCCCCTTGAAC
  CAAACCACATGGCATTTGACACTGGACCAGTTCAAACCACGGGCTGGGATTATAACCCTT
  GGCCCTGATCCAGGGAAGAAGGTCCTTGTAGGGGAAATTTTCAGTCTGAACATTAACATT
  TCCAAAGTTGGCACATTTTCTTTTAAATTTTGCAATTTTGTAAACAGAGTACTTTTTAAT
  GGTCTACTGCAAACTCTTGTATGCAGGCCACCAAGCCCGGGATTTTTCTTCCAGACAGAC
  CCTTGATGGCCCCCTCATTAAAGGAACACCATGGCGGCAGGGGGCCATTTAAATGGTACC
  CCTTGTCAACCGCCCTTTCCCTGTTTTAAACCGTTTTTTGAAACCTTAAGGGAAGACTTT
  GTCCTTCAAGCTACCAAAGGTTTTTTCCCTAACACCCTTTGCAAGGGGAGGTAAATCAAC
  CTNAACGGTCCCCCTTTTATCCCACATTTTTAGTTCCCTACCATTTGTGCAAAAAAAAAA
  AACCTTGCTCCTTTCCCGGGGAAAAAAAGGGGAATTGGACAATTGCTTTGGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000945
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000945.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000945.3, NP_000936.1
• RefSeq Size: 3111 bp
• RefSeq ORF: 513 bp
• Locus ID: 5534
• UniProt ID: P63098
• Cytogenetics: 2p14
• Domains: EFh
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.[UniProtKB/Swiss-Prot Function]