Calcineurin B (PPP3R1) (NM_000945) Human Untagged Clone

CAT#: SC322580

PPP3R1 (untagged)-Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Calcineurin B
• Synonyms: CALNB1; CNB; CNB1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322580 GTCAGCGCGAGCCGCTGCAGTGAGTCCGTCACGGCTCCGGCGCGAGCGCGAGGCTGCAGC
  CCCCGAGTTTCCCGGCCGTCTTCGCCCCCTCTCCCCCTCCTTTCTTCTTCTCTGCCTCTC
  CTGCCTCTCGCCGCTGCTCCTCCCGCGCTCTCCGGCTCTGAATCTCGACCTTAATTTATT
  TCCCCCTACCCTGCCCGCTCCCTCGCGTGCCCAATCGCCCGGCCGGCGCGGGCCCCGCGG
  CGCCGCCTCCCCTCCCCCACGCGCGCCCCCTCCCCGCCGGCGACCCGAGGGCCGCAGCTG
  GGCCGCCGCCGCCGTTTCCTGCGAGCCAGCCTGAGCGCAACACTTCTCCGAGCCAGCGAG
  CCAGCGAGCCGCCGACCCGCCGAGCAAAATGGGAAATGAGGCAAGTTATCCTTTGGAAAT
  GTGCTCACACTTTGATGCGGATGAAATTAAAAGGCTAGGAAAGAGATTTAAGAAGCTTGA
  TTTGGACAATTCTGGTTCTTTGAGTGTGGAAGAGTTCATGTCTCTGCCTGAGTTACAACA
  GAATCCTTTAGTACAGCGAGTAATAGATATATTCGACACAGATGGGAATGGAGAAGTAGA
  CTTTAAAGAATTCATTGAGGGCGTCTCTCAGTTCAGTGTCAAAGGAGATAAGGAGCAGAA
  ATTGAGGTTTGCTTTCCGTATCTATGACATGGATAAAGATGGCTATATTTCCAATGGGGA
  ACTCTTCCAGGTATTGAAGATGATGGTGGGGAACAATCTGAAAGATACACAGTTACAGCA
  AATTGTAGACAAAACCATAATAAATGCAGATAAGGATGGAGATGGAAGAATATCCTTTGA
  AGAATTCTGTGCTGTTGTAGGTGGCCTAGATATCCACAAAAAGATGGTGGTAGATGTGTG
  ACTCTTATCAGAGAGTACCACCCAACACTTTTGCTTTCTTCTCCATCTCTGAAGATCTGC
  TCAAGACGTCCAGCAATGCTCTCTGTGTATTTAAATGGAAGTATTTTTCTCTGTGAAGCC
  ACATTTTCCAACATGAGCCTCATGAAGCCAACTAAGTGTTATTGAACTGTAATTCTCTCA
  ATAACTCAGTGTAGCACTTTAAAGTCTGAAGGACAGCAACATGAAAAGAGCATATCAATG
  TGGTGGAGAAAGGGAAGGGGTTGGCTTTTTAATTTATTTTTCTTCATCTTTTATAACAAG
  AAAGTATCTATATATACATATGTAAATATTTATATATAGATATATGTAGCTTTCTATATA
  TGTAGTAGGTTGGCTTTAATTTAATATACTTGATTCAGAAACAAAACAATAGAGTACAAA
  AGTGCCAAGCAGAACATAAAACATCCTTACTTTTATTTCACACAGTTTTATATATAGATA
  GAAGACTGTACAATTTGAGCCGGGTGTTAGGCCAGCTATTTTCCTTTTCCTGTGCTTTCT
  CCTTTGAGAGATTGACAAAGCATTTGTTAACGTCCTATTATTTACCTTAATTACATTTTT
  GTAACAAAGGAGTCTGTAACTTTATTTATACTTATGAATATATCCAGGGACTACTTCTCA
  TTGCTGAGCAGCTTTTAATACACCTCTGCTTGAGGAGAAAGTCTAGTTCATTGCTACTGC
  CAAGAGCTAGTTCTTGTGTTCATATAGTAACTGCACAGGGCTTATAGCTGCTTCATTCTG
  CTACTTTGTAACTAGGAGCCATTGCATTTATTAAATGTCCCTCAGTAACGTTAAGTGCTA
  GTTGTGATTTTATACATAAAGGCCAGAAGCTGTCTGAGGCAATCATGATTGATTGTATGT
  ATCACTTACTGAAGAATACCTGAAGTGATCATGTAACTACTTATAAGGGATATCCATTTG
  TTTGATTACATGGGTAAATAATTTGTCATTAAACTTGTGTTTGAATCATGAATTCCCTTG
  TTTCAAAAGACTTGCAGCTAATCTAAAAAACTGGTGATATTTAATATGCATGTATGTATC
  TAAACACCCACATATATTTGTGGTTTAAGTGTGAGAAATCTTGCTAATCTATATGCCACA
  GAAGAGCAAAATTGTATCCAAATTTATGCCACTTAAATTTCTTTACCACGAGGGATAGAG
  CATGCATACTGGTTTTTTTTTCTTGATTTGCCCATATAATTGGTAATGGATAACTTAATA
  AATTTGTGTGATATAAAAGTAGTGTATCATGTTCTACTGTTTGATCTTATCCCTTCCCAT
  TTCTGCAAAGGTACTGTTAGCTGGAAGAAAAACATTTTGGTTAGCTTTGTATGACAGTAG
  TCTTTCCCTTTATAGTTTCTCTATAAAAACTGGTTTTAAAATCAGTGGAAAAGGGCAGGT
  TGAATCAAGGTGAATCAATCTGAAATTGAGCACACCTGCCTGCCATCGCTGTTCCTTCAA
  CTGAGTGCTGCACATCATGGGCTCTGTCTGTGAGAGAAAAATCCCGGTGCTTGGTGTCCT
  TGCATGACATGGAGTTTTGCATGTAGATCAATTTAAAATGTACCTCTTGTTTACATAATT
  TGCATAATTTTAAAAGATAATGTTGCCAAACTTTGGAAATGTTAATGTTCAGACTGAAAA
  TCTCCACTACATGTAACTTTCTTCCTCTGGATCAGTGGCATGGCTTATAATCCCAGCCAG
  TGGTTTGAACTGTTCCAGTGTCAACTGCCATGTGCTCTGCTTCAAGGGGGAACTAGCCTT
  TTGTGAATTTTTGTACATAAGTATTTGTTACAAATATTTTAGCAAATGCTTTCTATTTCT
  CTTGCTTGTGCATATCTTGGCTGGCGTTACAGAAAAATAGTGTAAACATTATTTCCTTAC
  CGGGGAATGAGGGTTTTTTCTTTTTCTTTTTTTTTTTTTTTTTTTTTAGTTTGTGTGTGG
  GGGTGGGTAAGGGAGGGGATGGTTTATGTTGAATGTTTAGTTTTTCTTCTGCATGATACG
  TCATGTTGTGGGATCTTTAGAAAACTTCATACTGTATGAATAAGAAAATAAAATATTTGA
  AACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000945
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000945.3, NP_000936.1
• RefSeq Size: 3111 bp
• RefSeq ORF: 513 bp
• Locus ID: 5534
• UniProt ID: P63098
• Cytogenetics: 2p14
• Domains: EFh
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.[UniProtKB/Swiss-Prot Function]