SIRT1 (NM_012238) Human Untagged Clone

CAT#: SC127917

SIRT1 (untagged)-Human sirtuin 1 (SIRT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIRT1
• Synonyms: SIR2; SIR2alpha; SIR2L1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012238, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGACGAGGCGGCCCTCGCCCTTCAGCCCGGCGGCTCCCCCTCGGCGGCGGGGGCCGACAGGGAGG
  CCGCGTCGTCCCCCGCCGGGGAGCCGCTCCGCAAGAGGCCGCGGAGAGATGGTCCCGGCCTCGAGCGGAG
  CCCGGGCGAGCCCGGTGGGGCGGCCCCAGAGCGTGAGGTGCCGGCGGCGGCCAGGGGCTGCCCGGGTGCG
  GCGGCGGCGGCGCTGTGGCGGGAGGCGGAGGCAGAGGCGGCGGCGGCAGGCGGGGAGCAAGAGGCCCAGG
  CGACTGCGGCGGCTGGGGAAGGAGACAATGGGCCGGGCCTGCAGGGCCCATCTCGGGAGCCACCGCTGGC
  CGACAACTTGTACGACGAAGACGACGACGACGAGGGCGAGGAGGAGGAAGAGGCGGCGGCGGCGGCGATT
  GGGTACCGAGATAACCTTCTGTTCGGTGATGAAATTATCACTAATGGTTTTCATTCCTGTGAAAGTGATG
  AGGAGGATAGAGCCTCACATGCAAGCTCTAGTGACTGGACTCCAAGGCCACGGATAGGTCCATATACTTT
  TGTTCAGCAACATCTTATGATTGGCACAGATCCTCGAACAATTCTTAAAGATTTATTGCCGGAAACAATA
  CCTCCACCTGAGTTGGATGATATGACACTGTGGCAGATTGTTATTAATATCCTTTCAGAACCACCAAAAA
  GGAAAAAAAGAAAAGATATTAATACAATTGAAGATGCTGTGAAATTACTGCAAGAGTGCAAAAAAATTAT
  AGTTCTAACTGGAGCTGGGGTGTCTGTTTCATGTGGAATACCTGACTTCAGGTCAAGGGATGGTATTTAT
  GCTCGCCTTGCTGTAGACTTCCCAGATCTTCCAGATCCTCAAGCGATGTTTGATATTGAATATTTCAGAA
  AAGATCCAAGACCATTCTTCAAGTTTGCAAAGGAAATATATCCTGGACAATTCCAGCCATCTCTCTGTCA
  CAAATTCATAGCCTTGTCAGATAAGGAAGGAAAACTACTTCGCAACTATACCCAGAACATAGACACGCTG
  GAACAGGTTGCGGGAATCCAAAGGATAATTCAGTGTCATGGTTCCTTTGCAACAGCATCTTGCCTGATTT
  GTAAATACAAAGTTGACTGTGAAGCTGTACGAGGAGATATTTTTAATCAGGTAGTTCCTCGATGTCCTAG
  GTGCCCAGCTGATGAACCGCTTGCTATCATGAAACCAGAGATTGTGTTTTTTGGTGAAAATTTACCAGAA
  CAGTTTCATAGAGCCATGAAGTATGACAAAGATGAAGTTGACCTCCTCATTGTTATTGGGTCTTCCCTCA
  AAGTAAGACCAGTAGCACTAATTCCAAGTTCCATACCCCATGAAGTGCCTCAGATATTAATTAATAGAGA
  ACCTTTGCCTCATCTGCATTTTGATGTAGAGCTTCTTGGAGACTGTGATGTCATAATTAATGAATTGTGT
  CATAGGTTAGGTGGTGAATATGCCAAACTTTGCTGTAACCCTGTAAAGCTTTCAGAAATTACTGAAAAAC
  CTCCACGAACACAAAAAGAATTGGCTTATTTGTCAGAGTTGCCACCCACACCTCTTCATGTTTCAGAAGA
  CTCAAGTTCACCAGAAAGAACTTCACCACCAGATTCTTCAGTGATTGTCACACTTTTAGACCAAGCAGCT
  AAGAGTAATGATGATTTAGATGTGTCTGAATCAAAAGGTTGTATGGAAGAAAAACCACAGGAAGTACAAA
  CTTCTAGGAATGTTGAAAGTATTGCTGAACAGATGGAAAATCCGGATTTGAAGAATGTTGGTTCTAGTAC
  TGGGGAGAAAAATGAAAGAACTTCAGTGGCTGGAACAGTGAGAAAATGCTGGCCTAATAGAGTGGCAAAG
  GAGCAGATTAGTAGGCGGCTTGATGGTAATCAGTATCTGTTTTTGCCACCAAATCGTTACATTTTCCATG
  GCGCTGAGGTATATTCAGACTCTGAAGATGACGTCTTATCCTCTAGTTCTTGTGGCAGTAACAGTGATAG
  TGGGACATGCCAGAGTCCAAGTTTAGAAGAACCCATGGAGGATGAAAGTGAAATTGAAGAATTCTACAAT
  GGCTTAGAAGATGAGCCTGATGTTCCAGAGAGAGCTGGAGGAGCTGGATTTGGGACTGATGGAGATGATC
  AAGAGGCAATTAATGAAGCTATATCTGTGAAACAGGAAGTAACAGACATGAACTATCCATCAAACAAATC
  ATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012238 unedited
  GACTTCCGCCCGCTTGCAGGGCGGAGCGTAGGGATGGAGGTGGTATAAGCGAGCTCGTGT
  AGTGATCGTCAGAATTTTGTACCAGGGCAGTTGGAAGATGGCGGACGAGGCGGCCCTCGC
  CCTTCAGCCCGGCGGCTCCCCCTCGGCGGCGGGGGCCGACAGGGAGGCCGCGTCGTCCCC
  CGCCGGGGAGCCGCTCCGCAAGAGGCCGCGGAGAGATGGTCCCGGCCTCGAGCGGAGCCC
  GGGCGAGCCCGGTGGGGCGGCCCCAGAGCGTGAGGTGCCGGCGGCGGCCAGGGGCTGCCC
  GGGTGCGGCGGCGGCGGCGCTGTGGCGGGAGGCGGAGGCAGAGGCGGCGGCGGCAGGCGG
  GGAGCAAGAGGCCCAGGCGACTGCGGCGGCTGGGGAAGGAGACAATGGGCCGGGCCTGCA
  GGGCCCATCTCGGGAGCCACCGCTGGCCGACAACTTGTACGACGAAGACGACGACGACGA
  GGGCGAGGAGGAGGAAGAGGCGGCGGCGGCGGCGATTGGGTACCGAGATAACCTTCTGTT
  CGGTGATGAAATTATCACTAATGGTTTTCATTCCTGTGAAAGTGATGAGGAGGATAGAGC
  CTCACATGCAAGCTCTAGTGACTGGACTCCAAGGCCACGGATAGGTCCATATACTTTTGT
  TCAGCAACATCTTATGATTGGCACAGATCCTCGAACAATTCTTAAAGATTTATTGCCGGA
  AACAATACCTCCACCTGAGTTGGATGATATGACACTGTGGCAGATTGTTATTATATCCTT
  TCAGAACCACCAAAAAGGAAAAAAAGAAAAGATATTAATACAATTGAGATGCTGTGAAAT
  TACTGCAGGAGTGCAAAAAAACTATAGTTCTAACTGCAGCTGGGGTGTCTGCTTTCATGT
  AGAATACCTGACTGTCAGTTCAAGGGATGGTATTTTAATGCTCGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012238 unedited
  GCTACTGCCTACGATACTTGTATTTAAAAGTTGAAGGAAGAAAACCAGTCATTACACACA
  GTCTACAAAACATATGCCAGTAAATTACATAAAAGACTATGTACAATATAAAAAGAGCTG
  AAAACAGTCTTCACTGTAAAAATAATTTAAAACAAACTTTTCAATTTAAAATATCATCTA
  TAGCACACAAACATCATGCAAATGGAAAACTAAATATACTGCATTCTTTAGTGTAGCCAA
  ATAAATTCAGATTGAGACATCTTATAAGTAGGGAAATGGCCATTCAATACGATTTTTTTC
  TCTGGCAGTAATGGTCCTAGCTGGGTGTTTTATGCATAAAGAACAGCTATATTTCAAACC
  CTTTTTATTGTAATAAATACTAAAGCAACAGAGGAATACTTTATTAATTTAGGAGTGATG
  TTCAAAAATGGTCTGAAAAATAAATGCTTACTGTGCAATTTCATAAAGTATGAACTTCTT
  GAAAGACTAGAAGCTTTTGCAGCTGAGAAAGTTGATCTCTAGTTTTAAGGCAGGCTAAGC
  TTTTAAATAAAGATAAATTATAAGAACTAGTTTCATTCATTAACTATTCTGCTATTACAA
  GTTACATCATCATGTTCATCTCCATAATACTAGGGCTAGCAGTTTGGTATTTAACACCAA
  ATCCTCCCAATGGGAGACATGTACTTTCTGGCTGAATTACAATTAACATCTTATTACTTT
  GGAACTGTATAATCAGGGGCCTGTTGCTCTCCTCATTAATGGAAAACAGCAGTCCAAAAT
  ATTACACTCTCCCCAGTAGAAGTACCATTATTATAGATTCAGAAAGGCTTTGCATATTCA
  CATTTTTGCAGTTTAATACTTGTGAAGATCAATGCAAGCTCTACACAGTGATAGTATTTA
  AACACATGCAATATTTCAAACTAAGGGGATCCAGCAGCCACTAATGTTTTCTAAGCACAC
  TTGAAAACATTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012238
• Insert Size: 4100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012238.3, NP_036370.2
• RefSeq Size: 4107 bp
• RefSeq ORF: 2244 bp
• Locus ID: 23411
• UniProt ID: Q96EB6
• Cytogenetics: 10q21.3
• Domains: SIR2
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).