PDI (PDIA2) (NM_006849) Human Untagged Clone

CAT#: SC127860

PDIA2 (untagged)-Human protein disulfide isomerase family A, member 2 (PDIA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDI
• Synonyms: PDA2; PDI; PDIP; PDIR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006849, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCGCCAGCTTCTGCCTGTACTGCTGCTGCTGCTGCTCAGGGCTTCGTGCCCATGGGGTCAGGAAC
  AGGGAGCGAGGAGCCCCTCGGAGGAGCCTCCAGAGGAGGAAATCCCCAAGGAGGATGGGATCTTGGTGCT
  GAGCCGCCACACCCTGGGCCTGGCCCTGCGGGAGCACCCTGCCCTGCTGGTGGAATTCTATGCCCCGTGG
  TGTGGGCACTGCCAGGCCCTGGCCCCCGAGTACAGCAAGGCAGCTGCCGTGCTCGCGGCCGAGTCAATGG
  TGGTCACGCTGGCCAAGGTGGATGGGCCCGCGCAGCGCGAGCTGGCTGAGGAGTTTGGTGTGACGGAGTA
  CCCTACGCTCAAGTTCTTCCGCAATGGGAACCGCACGCACCCGGAGGAGTACACAGGACCACGGGACGCT
  GAGGGCATTGCCGAGTGGCTGCGACGGCGGGTGGGGCCCAGTGCCATGCGGCTGGAGGACGAGGCGGCCG
  CCCAGGCGCTGATCGGTGGCCGGGACCTAGTGGTCATTGGCTTCTTCCAGGACCTGCAGGACGAGGACGT
  GGCCACCTTCTTGGCCTTGGCCCAGGACGCCCTGGACATGACCTTTGGCCTCACAGACCGGCCGCGGCTC
  TTTCAGCAGTTTGGCCTCACCAAGGACACTGTGGTTCTCTTCAAGAAGTTTGATGAGGGGCGGGCAGACT
  TCCCCGTGGACGAGGAGCTTGGCCTGGACCTGGGGGATCTGTCGCGCTTCCTGGTCACACACAGCATGCG
  CCTGGTCACGGAGTTCAACAGCCAGACGTCTGCCAAGATCTTCGCGGCCAGGATCCTCAACCACCTGCTG
  CTGTTTGTCAACCAGACGCTGGCTGCGCACCGGGAGCTCCTAGCGGGCTTTGGGGAGGCAGCTCCCCGCT
  TCCGGGGGCAGGTGCTGTTCGTGGTGGTGGACGTGGCGGCCGACAATGAGCACGTGCTGCAGTACTTTGG
  ACTCAAGGCTGAGGCAGCCCCCACTCTGCGCTTGGTCAACCTTGAAACCACTAAGAAGTATGCGCCTGTG
  GATGGGGGCCCTGTCACCGCAGCGTCCATCACTGCTTTCTGCCATGCAGTCCTCAACGGCCAAGTCAAGC
  CCTATCTCCTGAGCCAGGAGATACCCCCTGATTGGGATCAGCGGCCAGTTAAGACCCTCGTGGGCAAGAA
  TTTTGAGCAGGTGGCTTTTGACGAAACCAAGAATGTGTTTGTCAAGTTCTATGCCCCGTGGTGCACCCAC
  TGCAAGGAGATGGCCCCTGCCTGGGAGGCATTGGCTGAGAAGTACCAAGACCACGAGGACATCATCATTG
  CTGAGCTGGATGCCACGGCCAACGAGCTGGATGCCTTCGCTGTGCACGGCTTCCCTACTCTCAAGTACTT
  CCCAGCAGGGCCAGGTCGGAAGGTGATTGAATACAAAAGCACCAGGGACCTGGAGACTTTCTCCAAGTTC
  CTGGACAACGGGGGCGTGCTGCCCACGGAGGAGCCCCCGGAGGAGCCAGCAGCCCCGTTCCCGGAGCCAC
  CGGCCAACTCCACTATGGGGTCCAAGGAGGAACTGTAG
  
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006849
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006849.2, NP_006840.2
• RefSeq Size: 1726 bp
• RefSeq ORF: 1578 bp
• Locus ID: 64714
• UniProt ID: Q13087
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular estradiol levels. [provided by RefSeq, Sep 2017]