ASAP1 (NM_018482) Human Untagged Clone

CAT#: SC127851

ASAP1 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASAP1
• Synonyms: AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018482, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGATCTTCAGCCTCCAGGCTCTCCAGTTTTTCGTCGAGAGATTCACTATGGAATCGGATGCCGGACC
  AGATCTCTGTCTCGGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCAC
  CACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCC
  CTTCAGAAAGTGAAGAAGTCTGTAAAAGCAATATATAATTCTGGTCAAGATCATGTACAAAATGAAGAAA
  ACTATGCACAAGTTCTTGATAAGTTTGGGAGTAATTTTTTAAGTCGAGACAACCCCGACCTTGGCACCGC
  GTTTGTCAAGTTTTCTACTCTTACAAAGGAACTGTCCACACTGCTGAAAAATCTGCTCCAGGGTTTGAGC
  CACAATGTGATCTTCACCTTGGATTCTTTGTTAAAAGGAGACCTAAAGGGAGTCAAAGGAGATCTCAAGA
  AGCCATTTGACAAAGCCTGGAAAGATTATGAGACAAAGTTTACAAAAATTGAGAAAGAGAAAAGAGAGCA
  CGCAAAACAACATGGGATGATCCGCACAGAGATAACAGGAGCTGAGATTGCGGAAGAAATGGAGAAGGAA
  AGGCGCCTCTTTCAGCTCCAAATGTGTGAATATCTCATTAAAGTTAATGAAATCAAGACCAAAAAGGGTG
  TGGATCTGCTGCAGAATCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAAGATGGCTTGAAAAC
  AGCTGATAAGTTGAAACAGTACATTGAAAAACTGGCTGCTGATTTATATAATATAAAACAGACCCAGGAT
  GAAGAAAAGAAACAGCTAACTGCACTCCGAGACTTAATAAAATCCTCTCTTCAACTGGATCAGAAAGAAG
  ATTCTCAGAGCCGGCAAGGAGGATACAGCATGCATCAGCTCCAGGGCAATAAGGAATATGGCAGTGAAAA
  GAAGGGGTACCTGCTAAAGAAAAGTGACGGGATCCGGAAAGTATGGCAGAGGAGGAAGTGTTCAGTCAAG
  AATGGGATTCTGACCATCTCACATGCCACATCTAACAGGCAACCAGCCAAGTTGAACCTTCTCACCTGCC
  AAGTAAAACCTAATGCCGAAGACAAAAAATCTTTTGACCTGATATCACATAATAGAACATATCACTTTCA
  GGCAGAAGATGAGCAGGATTATGTAGCATGGATATCAGTATTGACAAATAGCAAAGAAGAGGCCCTAACC
  ATGGCCTTCCGTGGAGAGCAGAGTGCGGGAGAGAACAGCCTGGAAGACCTGACAAAAGCCATTATTGAGG
  ATGTCCAGCGGCTCCCAGGGAATGACATTTGCTGCGATTGTGGCTCATCAGAACCCACCTGGCTTTCAAC
  CAACTTGGGTATTTTGACCTGTATAGAATGTTCTGGCATCCATAGGGAAATGGGGGTTCATATTTCTCGC
  ATTCAGTCTTTGGAACTAGACAAATTAGGAACTTCTGAACTCTTGCTGGCCAAGAATGTAGGAAACAATA
  GTTTTAATGATATTATGGAAGCAAATTTACCCAGCCCCTCACCAAAACCCACCCCTTCAAGTGATATGAC
  TGTACGAAAAGAATATATCACTGCAAAGTATGTAGATCATAGGTTTTCAAGGAAGACCTGTTCAACTTCA
  TCAGCTAAACTAAATGAATTGCTTGAGGCCATCAAATCCAGGGATTTACTTGCACTAATTCAAGTCTATG
  CAGAAGGGGTAGAGCTAATGGAACCACTGCTGGAACCTGGGCAGGAGCTTGGGGAGACAGCCCTTCACCT
  TGCCGTCCGAACTGCAGATCAGACATCTCTCCATTTGGTTGACTTCCTTGTACAAAACTGTGGGAACCTG
  GATAAGCAGACGGCCCTGGGAAACACAGTTCTACACTACTGTAGTATGTACAGTAAACCTGAGTGTTTGA
  AGCTTTTGCTCAGGAGCAAGCCCACTGTGGATATAGTTAACCAGGCTGGAGAAACTGCCCTAGACATAGC
  AAAGAGACTAAAAGCTACCCAGTGTGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAATCCACAC
  GTCCACGTAGAATATGAGTGGAATCTTCGACAGGAGGAGATAGATGAGAGCGATGATGATCTGGATGACA
  AACCAAGCCCTATCAAGAAAGAGCGCTCACCCAGACCTCAGAGCTTCTGCCACTCCTCCAGCATCTCCCC
  CCAGGACAAGCTGGCACTGCCAGGATTCAGCACTCCAAGGGACAAACAGCGGCTCTCCTATGGAGCCTTC
  ACCAACCAGATCTTCGTTTCCACAAGCACAGACTCGCCCACATCACCAACCACGGAGGCTCCCCCTCTGC
  CTCCTAGGAACGCCGGGAAAGGTCCAACTGGCCCACCTTCAACACTCCCTCTAAGCACCCAGACCTCTAG
  TGGCAGCTCCACCCTATCCAAGAAGAGGCCTCCTCCCCCACCACCCGGACACAAGAGAACCCTATCCGAC
  CCTCCCAGCCCACTACCTCATGGGCCCCCAAACAAAGGCGCAGTTCCTTGGGGTAACGATGGGGGTCCAT
  CCTCTTCAAGTAAGACTACAAACAAGTTTGAGGGACTATCCCAGCAGTCGAGCACCAGTTCTGCAAAGAC
  TGCCCTTGGCCCAAGAGTTCTTCCTAAACTACCTCAGAAAGTGGCACTAAGGAAAACAGATCATCTCTCC
  CTAGACAAAGCCACCATCCCGCCCGAAATCTTTCAGAAATCATCACAGTTGGCAGAGTTGCCACAAAAGC
  CACCACCTGGAGACCTGCCCCCAAAGCCCACAGAACTGGCCCCCAAGCCCCAAATTGGAGATTTGCCGCC
  TAAGCCAGGAGAACTGCCCCCCAAACCACAGCTGGGGGACCTGCCACCCAAACCCCAACTCTCAGACTTA
  CCTCCCAAACCACAGATGAAGGACCTGCCCCCCAAACCACAGCTGGGAGACCTGCTAGCAAAATCCCAGA
  CTGGAGATGTCTCACCCAAGGCTCAGCAACCCTCTGAGGTCACACTGAAGTCACACCCATTGGATCTATC
  CCCAAATGTGCAGTCCAGAGACGCCATCCAAAAGCAAGCATCTGAAGACTCCAACGACCTCACGCCTACT
  CTGCCAGAGACGCCCGTACCACTGCCCAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGAGTGAAGA
  CCATTTATGACTGCCAGGCAGACAACGATGACGAGCTCACATTCATCGAGGGAGAAGTGATTATCGTCAC
  AGGGGAAGAGGACCAGGAGTGGTGGATTGGCCACATCGAAGGACAGCCTGAAAGGAAGGGGGTCTTTCCA
  GTGTCCTTTGTTCATATCCTGTCTGACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018482 unedited
  AGGGCGGCCCGCGAATTCGGCACGAGGCGCCCCTTCCTGCGCCGCGCTCGGCTCCCGCGC
  CTCGCCTGGCCTGGCCGCTGCCGCGGGCGAGCACTGTGCGCTGGGCAGCCCCGGGCCCGC
  GCCTCCCGCCCAGCCGGCGCTTCCCGGTCGCAGTTCGCTTTCCCACCCGAGAGCCGCGAG
  CGCCCGGCCGAACCCGCGATGGAATAATGCCCAGCGGCCCGCCAGGTCCCGGTCGTTTTC
  TGATGTGACGGCTGAGACATGAGATCTTCAGCCTCCAGGCTCTCCAGTTTTTCGTCGAGA
  GATTCACTATGGAATCGGATGCCGGACCAGATCTCTGTCTCGGAGTTCATCGCCGAGACC
  ACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCACCACGCGGCTGCACAACTGCAGG
  AACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCCCTTCAGAAAGTG
  AAGAAGTCTGTAAAAGCAATATATAATTCTGGTCAAGATCATGTACAAAATGAAGAAAAC
  TATGCACAAGTTCTTGATAAGTTTGGGAGTAATTTTCTAAGTCGAGACAACCCCGACCTT
  GGCACCGCGTTTGTCAAGTTTTCTACTCTTACCAAGGAACTGTCCACACTTGCTGAAAAA
  ATCTGCTCCCAGGGTTTGAACCACCAATGTGATCTTTCCCCTTGGGATTCTTTTGTTAAA
  AAGGAAAACCTTAAAAGCGATTCCAAGAGAGATCTTAAAGAATGCCATTTGGACCAAAGC
  CTGGAAAGACTATGAGACAAAGCTTCCAAAAACGGAGAAAGAAAAAAGAGAGCACGCAAA
  ACACTTGTGATGACCGGCCAGAGATACCAGATCCGTCATCGCCGTAAAATGAGAAAGAAA
  GGCGCCTCTTTCAACCCAAAGGGTGAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018482 unedited
  CATTGAAAAATTATACTCAGGACCACAGTGAAAAAAACAGCATCTGTTAATGCCAAGCTT
  ATAAATAGTGCTAAATGTAATGAACTCGGGNACACTCGACCAACCTCATATGCAGACTTC
  CATCCCGGACACTGGTGTGGCTTCAGTACTGAAGCTGCTAAAGCTGCCAATCCCAACCCA
  TCATGTCAACTGGTTCCTCATAGCTCTGTTTGGTGTGGAAATTCACATGTGCCCTGACAC
  TGACGAAGCAGTTGCTTAAAATCACTTTCCAATAACATCTGATAAAATATTTTGCAAGTT
  TGCCATGCAAAGATTATTTATTAAGAGGCTATTCAAAGTTTGTACAACAACCACTTAAAC
  AGAACTCACTTAATATTCACTAGATGCACTGTATCGATAGGAATAGGGACTTTTCCTAAA
  GGTTGACTTGGGAATTGTGCTTCTGTGAAATTAACATCTCTCACTGATTGACAAGATTCT
  ATACTTAAAAATATTAATTCTCTGTGCTGACGCCAATATAGCATTTAAACAGATGCATCC
  CACATTGATAATTATCGCTGTACTCATGTAAAAATCCCATTGACCGTGAAACTACACCAG
  TGAATAACTTTCCAAAAATAAGACTGATGTGAAAGACTGACTTCCCAAGTTCTCAAAGTT
  CCGAAAACCCTGTCCATGTATTACAAGACACTAATATGGAAAAAGTGCTTTTTCTAGTGC
  AAGCCTTACACGATTCCAAACACATGGCCCCACACTTGAATCTGATTTGAACGGAGTCTG
  ATATATGGACCAGTTTCCTTAAATTTGCGCCCACACCATTTCGCGCGAAATTTCCCTTGC
  GCCCTAGTAACAGCCCTTAAATCACGCGACGGACACCGCCACAAGGTTTAAATCCCCCAC
  TCGATTTGTTATTCCGCCACCCCAATTATTTTTTGTTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018482
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018482.2, NP_060952.2
• RefSeq Size: 6042 bp
• RefSeq ORF: 3390 bp
• Locus ID: 50807
• UniProt ID: Q9ULH1
• Cytogenetics: 8q24.21-q24.22
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.