Aromatase (CYP19A1) (NM_000103) Human Untagged Clone

SKU SC127848

CYP19A1 (untagged)-Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Aromatase
• Synonyms: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127848 sequence for NM_000103 edited (data generated by NextGen Sequencing); ATGGTTTTGGAAATGCTGAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCC; ATGCCTGCTGCCACCATGCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAAT; TATGAGGGCACATCCTCAATACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATC; TCCCACGGCAGATTCCTGTGGATGGGGATCGGCAGTGCCTGCAACTACTACAACCGGGTA; TATGGAGAATTCATGCGAGTCTGGATCTCTGGAGAGGAAACACTCATTATCAGCAAGTCC; TCAAGTATGTTCCACATAATGAAGCACAATCATTACAGCTCTCGATTCGGCAGCAAACTT; GGGCTGCAGTGCATCGGTATGCATGAGAAAGGCATCATATTTAACAACAATCCAGAGCTC; TGGAAAACAACTCGACCCTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATG; GTCACAGTCTGTGCTGAATCCCTCAAAACACATCTGGACAGGTTGGAGGAGGTGACCAAT; GAATCGGGCTATGTGGACGTGTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTAAC; ACGCTCTTCTTGAGGATCCCTTTGGACGAAAGTGCTATCGTGGTTAAAATCCAAGGTTAT; TTTGATGCATGGCAAGCTCTCCTCATCAAACCAGACATCTTCTTTAAGATTTCTTGGCTA; TACAAAAAGTATGAGAAGTCTGTCAAGGATTTGAAAGATGCCATAGAAGTTCTGATAGCA; GAAAAAAGACGCAGGATTTCCACAGAAGAGAAACTGGAAGAATGTATGGACTTTGCCACT; GAGTTGATTTTAGCAGAGAAACGTGGTGACCTGACAAGAGAGAATGTGAACCAGTGCATA; TTGGAAATGCTGATCGCAGCTCCTGACACCATGTCTGTCTCTTTGTTCTTCATGCTATTT; CTCATTGCAAAGCACCCTAATGTTGAAGAGGCAATAATAAAGGAAATCCAGACTGTTATT; GGTGAGAGAGACATAAAGATTGATGATATACAAAAATTAAAAGTGATGGAAAACTTCATT; TATGAGAGCATGCGGTACCAGCCTGTCGTGGACTTGGTCATGCGCAAAGCCTTAGAAGAT; GATGTAATCGATGGCTACCCAGTGAAAAAGGGGACAAACATTATCCTGAATATTGGAAGG; ATGCACAGACTCGAGTTTTTCCCCAAACCCAATGAATTTACTCTTGAAAATTTTGCAAAG; AATGTTCCTTATAGGTACTTTCAGCCATTTGGCTTTGGGCCCCGTGGCTGTGCAGGAAAG; TACATCGCCATGGTGATGATGAAAGCCATCCTCGTTACACTTCTGAGACGATTCCACGTG; AAGACATTGCAAGGACAGTGTGTTGAGAGCATACAGAAGATACACGACTTGTCCTTGCAC; CCAGATGAGACTAAAAACATGCTGGAAATGATCTTTACCCCAAGAAACTCAGACAGGTGT; CTGGAACACTAG; ; Clone variation with respect to NM_000103.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000103 unedited; AGTTCACAATTGTATACGACNACTATAGGCGGCCGCGNAATTCGCACCAGGGCAAACAGG; AAGGTGAAGAAGAACTTATCCTATCAGGACGGAAGGTCCTGTGCTCGGGATCTTCCAGAC; GTCGCGACTCTAAATTGCCCCCTCTGAGGTCAAGGAACACAAGATGGTTTTGGAAATGCT; GAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCCATGCCTGCTGCCACCAT; GCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAATTATGAGGGCACATCCTC; AATACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATCTCCCACGGCAGATTCCT; GTGGATGGGGATCGGCAGTGCCTGCAACTACTACAACCGGGTATATGGAGAATTCATGCG; AGTCTGGATCTCTGGAGAGGAAACACTCATTATCAGCAAGTCCTCAAGTATGTTCCACAT; AATGAAGCACAATCATTACAGCTCTCGATTCGGCAGCAAACTTGGGCTGCAGTGCATCGG; TATGCATGAGATAGGCATCATATTTAACAACAATCCAGAGCTCTGGAAAACAACTCGACC; CTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATGGTCACAGTCTGTGCTGA; ATCCCTCANAACACATCTGGACAGGTTGGAGGATGTGACCAATGAATCGGGCTTGTGGNA; CGTGTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTACACGCTCTTCTTGANGATC; CCTTTGGACGAAGTGCTATCGTGGNTAAATNCAAGGTATTNTGATGCATGGCAGCTCTCT; CATCAACAGAATCTCTTTAGATTTCTGCTTACAAAGTTGANAAGCGTCAAGATTGAAGAT; CCCTAAAGTCTGATACGAAAAGAGCAGATTNCCGAAAGACTGGANATGATGACTTGNCCT; GGATGTTTACNAAAACTGTGACGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000103 unedited; CGTTTCTTTTCCCCCCCCCCCCAATGCTGGGGAGAAGAGCCAATTGTGAGTGTTTGAATG; ACATTCAGTAGAGATAGTTATATTGGCTATATTACACAAGTAAAGTGGTGTTTGGAAAGT; TCCTACATTCATTTGATTTCCTTTAGAAAGCGAATTCCAAGGTGTGCAATTTATCCATGA; CCACTTACATTGATTCATGGAACCAAAATATGTTAGATTCCCTCAGGTAACCGGTTAAAA; TTTTGAGGTTGGAGTTAAAAAAATACTTCAGGGGGGTTTTACTTTTGGACCACCGTTTTT; TTATTGGAGTGGGGGCCCCCTAGGATCTTTGGGGGCAGGGCGAAAAAAAAAGGGGGAAAA; TATTTTCCGGGGGGAAAGAATAATAGGGGGTTAAAAGAGAGAGGCCCCCTCTTAAGAGAG; TGGACACCCCCCTGGGGGGGGGGGATTTTTTTTTTCCCCAACAAACCAATCCCCTTGTTA; TAAAGAAAACACCAAAATGGGGGCAGAAAACACGCCGGTTCCTTCTGGTGTGGCGCCCCC; CCTCTTATTTTGGCACAACACAAAAAAGGGGGAGCACACCCCCCCCAGAGCTTCTTTTTT; TTATTTATCTTAAAGAGGGCGCGCGGCGCGAAGATGTCTTTCCTTTCCTAAAAAAGAAAG; AGGGCCGGCGCCAATATTAATCCCCCCCCCCCCTCTCTTAAATATATTTTTTGCCCCCGT; GGGGGATAATATTAAAATTTGTTTCTCCCCCCATCCACACCAGCCACACCACACAAAATA; TTGTCTCANTGTCGCCTCGGGCCGTGCGGCGCGTCTACGACCAACACCGGCGGCGGAGAA; AACACTTCTGCCTCAATAAATTTCTCCCCCCCCCCCCCACATTTATTGTTCCACAAAACA; AAAAGGTTTGTTTTCTTTTTTTTTAAATCGGCGCCAAGACCCGAACGTNTTTTACCACCT; CCTAAACCACCATAAAGATCTTTTTTCGTTCCCCCCCCCCCCCCACACAAAAAAAAATTG; TTCCTTCTCTCTCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000103
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000103.2, NP_000094.2
• RefSeq Size: 3007 bp
• RefSeq ORF: 1512 bp
• Locus ID: 1588
• UniProt ID: P11511
• Cytogenetics: 15q21.2
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]; Transcript Variant: This variant (1) represents the use of an alternate promoter and contains an alternate 5' exon (I.1), compared to variant 4. All variants encode the same protein. The 5' end of this transcript contains long terminal repeat (LTR) sequence, initiates from an LTR promoter and results in placenta-specific expression. (PMID: 19577618). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.