PPAR delta (PPARD) (NM_006238) Human Untagged Clone

SKU SC127818

PPARD (untagged)-Human peroxisome proliferator-activated receptor delta (PPARD), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PPAR delta
• Synonyms: FAAR; NR1C2; NUC1; NUCI; NUCII; PPARB
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006238 edited; ATGGAGCAGCCACAGGAGGAAGCCCCTGAGGTCCGGGAAGAGGAGGAGAAAGAGGAAGTG; GCAGAGGCAGAAGGAGCCCCAGAGCTCAATGGGGGACCACAGCATGCACTTCCTTCCAGC; AGCTACACAGACCTCTCCCGGAGCTCCTCGCCACCCTCACTGCTGGACCAACTGCAGATG; GGCTGTGACGGGGCCTCATGCGGCAGCCTCAACATGGAGTGCCGGGTGTGCGGGGACAAG; GCATCGGGCTTCCACTACGGTGTTCATGCATGTGAGGGGTGCAAGGGCTTCTTCCGTCGT; ACGATCCGCATGAAGCTGGAGTACGAGAAGTGTGAGCGCAGCTGCAAGATTCAGAAGAAG; AACCGCAACAAGTGCCAGTACTGCCGCTTCCAGAAGTGCCTGGCACTGGGCATGTCACAC; AACGCTATCCGTTTTGGTCGGATGCCGGAGGCTGAGAAGAGGAAGCTGGTGGCAGGGCTG; ACTGCAAACGAGGGGAGCCAGTACAACCCACAGGTGGCCGACCTGAAGGCCTTCTCCAAG; CACATCTACAATGCCTACCTGAAAAACTTCAACATGACCAAAAAGAAGGCCCGCAGCATC; CTCACCGGCAAAGCCAGCCACACGGCGCCCTTTGTGATCCACGACATCGAGACATTGTGG; CAGGCAGAGAAGGGGCTGGTGTGGAAGCAGTTGGTGAATGGCCTGCCTCCCTACAAGGAG; ATCAGCGTGCACGTCTTCTACCGCTGCCAGTGCACCACAGTGGAGACCGTGCGGGAGCTC; ACTGAGTTCGCCAAGAGCATCCCCAGCTTCAGCAGCCTCTTCCTCAACGACCAGGTTACC; CTTCTCAAGTATGGCGTGCACGAGGCCATCTTCGCCATGCTGGCCTCTATCGTCAACAAG; GACGGGCTGCTGGTAGCCAACGGCAGTGGCTTTGTCACCCGTGAGTTCCTGCGCAGCCTC; CGCAAACCCTTCAGTGATATCATTGAGCCTAAGTTTGAATTTGCTGTCAAGTTCAACGCC; CTGGAACTTGATGACAGTGACCTGGCCCTATTCATTGCGGCCATCATTCTGTGTGGAGAC; CGGCCAGGCCTCATGAACGTTCCACGGGTGGAGGCTATCCAGGACACCATCCTGCGTGCC; CTCGAATTCCACCTGCAGGCCAACCACCCTGATGCCCAGTACCTCTTCCCCAAGCTGCTG; CAGAAGATGGCTGACCTGCGGCAACTGGTCACCGAGCACGCCCAGATGATGCAGCGGATC; AAGAAGACCGAAACCGAGACCTCGCTGCACCCTCTGCTCCAGGAGATCTACAAGGACATG; TACTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006238 unedited; TTGTTCCCCCGCCCGTTGCCGCATATGGGCGGTAGCGTGTACGGTGGGAGGTCTATATAA; GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG; CGAATTCGGCACGAGGGAATTCTGCGGAGCCTGCGGGACGGCGGCGGTGGCGCCGTATGC; AGCCGGGACAGTGTTGTACAGTGTTTTGGGCATGCACGTGATACTCACACAGTGGCTTCT; GCTCACCAACAGATGAAGACAGATGCACCAACGAGGAGGTGAGGAGCGCCTCTTTCCGGC; CGCCATCACATCTAGGAAGGAGGAGCGTCTCTGCCCGGCCGCCCATCGTCTGAGATGTGG; GGAGCGCCTCTGCCCCGCCGCCCCGTCTGGGATGTGAGGAGCACCTCTGCCCGGCCACGA; CCCCGTCTGGGAGGTGAGGAGCATCTCTGCCCCGCCGCCCCGTCTGAGAAGTGAGGAGAC; CCTCTGCCCGGCAACCACCCCGTCTGAGAAGTGAGGAGACCCTCCACCCGGCAGCTGCCC; CGTCTGAGAAATGAGGAGCCTCTCCGCCCGGCAGCCACCCCGTCTGTGAAGCTGATGGGA; ACCACCCTGTAGAGGTCCATCTGCGTTCAGACCCAGACGATGCCAGAGCTATGACTGNGC; CTGCATGTGTGGCGCCGAGGGGAGATCATCCATGGAGCAGCCACAGGAGGAAGCCCCTGA; GGTCCCGGAAGAGGATGAGAAAGAGGAAGTGCAGACGCAGAAGGAGCCCCAGAGCTCAAT; GGGGGACCACAGGATGCACTTTCTTTCAGCAGCTACACAGACCTCTCCCGGAGCTCCTCG; NCACCCTCACTGCTGGACCAACTGCAGATGGACTGTGACGGGGCCTATGCGGCAGCCTCA; CATGGAGTGCCCGGCTGTGCCGGGACATGCATCGGACTTCACTACGGTGTTATGCATGTG; AGGGGCAAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006238 unedited; GGGCTCCGTTTGGTTNCNCCATTAATCTTCAACGTGNTNCGANCAGTNCTGGTGTCACAC; TATCTTATATTACAAAACACAGGCAGTCTGGGGCTCCTAGCAAAAATATACATTTCGAAT; TTTGGAGATTGTTCAGACACTGAGAAGAGCTGTATCCTCAGCACCAGACCCGGGTCGGGG; CAGGGACGCGGCATGTGGTCGGTGTGGGGGAGGTGGCCTCCACCTCTGTCCCTTCCTTTT; TTTTTTCTGCTGGTCCTCCTCCCCTCCTTCCTCCTCGGTCCGTGGTCGTCCCCGCCAATC; GTGCCGCCCCCTCCCGCCCCGTTCCTTCCCTGGGATGCTGCACCGCCCGTCCGGCGCCGG; GCTGGCCTCCTCTCCCCCTGGTCTTTTCTTTATGGCTCTCCGGCGCGGCGCGTCCCTGCC; CGCCCCCCGTCTCTTGCGTTCCGCCCCCTGCTTGTCGTCCCTGTGCGCCCCCGCTCCTGC; GTCCTTCGCCCCTGCCCCTGTTGTCGTGCATGTGGAATCCCCCGGCTTCGTCGTGCTTTT; CTCCTTCTGCCCNCGTCTGCCCTGCGCTGCTGTCCTTCGTCCGTCTCCCGTCCCTCTCTC; TTTCGCTCCTCCCTCTCCCGCGCCTTTTCTGTGCCCCCCCGGCCCCGCCCCGTTTCCCGC; GTGTTCGTCGTCCCCCTCGCGCGCGTGCTCATTGCCCCTCCTCCCCGTTCCACCGGGCGC; ATCCCCCGCGCTCTCGGTTCTCCCCGGCGCCTCCTTCGTTGCCGAGTTTTTGTCTCCCGG; CGCGTCCTCCTCCTTGTGTTCGTGTTCGCTTCCTTTTCGTGGATTTAGTGTTTCCGACCG; CTCGCCCCCCCTCTCGTCCCCTCCCCCTTCCTTCCCTTTCGGCGTTTCTCCCTGAACTCC; AGCTCGTTTTTTGCTCGGCGGCGCGCTTCCTTGTGCATATNTCTCTTTCTTTTTTGCTTG; GTCGTTCCCTCTCGCCCGCTTATTTTTCCCGTTTTCGCCTCGCCCGTTCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006238
• Insert Size: 1326 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_006238.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006238.2, NP_006229.1
• RefSeq Size: 3328 bp
• RefSeq ORF: 1326 bp
• Locus ID: 5467
• UniProt ID: Q03181
• Cytogenetics: 6p21.31
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, PPAR signaling pathway, Wnt signaling pathway
• Summary: This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. The encoded protein is thought to function as an integrator of transcriptional repression and nuclear receptor signaling. It may inhibit the ligand-induced transcriptional activity of peroxisome proliferator activated receptors alpha and gamma, though evidence for this effect is inconsistent. Expression of this gene in colorectal cancer cells may be variable but is typically relatively low. Knockout studies in mice suggested a role for this protein in myelination of the corpus callosum, lipid metabolism, differentiation, and epidermal cell proliferation. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2017]; Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 3 encode the same isoform (1).