OGDH (NM_002541) Human Untagged Clone

CAT#: SC127702

OGDH (untagged)-Human oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (OGDH), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OGDH
• Synonyms: AKGDH; E1k; KGD1; OGDC; OGDH2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127702 sequence for NM_002541 edited (data generated by NextGen Sequencing)
  ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTGAGGCCATTGACGGCTTCCCAGACTGTT
  AAGACATTTTCACAAAACAGACCAGCAGCAGCTAGGACATTTCAACAGATTCGGTGCTAT
  TCTGCACCTGTTGCTGCTGAGCCCTTTCTCAGTGGGACTAGTTCGAACTATGTGGAGGAG
  ATGTACTGTGCTTGGCTGGAAAACCCCAAAAGTGTACATAAGTCATGGGACATTTTTTTT
  CGCAACACGAATGCCGGAGCCCCACCGGGCACTGCCTACCAGAGTCCCCTTCCCCTGAGC
  CGAGGCTCCCTGGCTGCTGTGGCCCATGCACAGTCCCTGGTAGAAGCACAGCCCAACGTG
  GACAAGCTCGTGGAGGACCACCTGGCAGTGCAGTCGCTCATCAGGGCATATCAGATACGA
  GGGCACCATGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCC
  GTGCCCGCTGACATTATCTCATCCACAGACAAACTTGGGTTCTATGGCCTGGATGAGTCT
  GACCTCGACAAGGTCTTCCACTTGCCCACCACCACTTTCATCGGGGGACAGGAATCAGCA
  CTTCCTCTGCGGGAGATCATCCGTCGGCTGGAGATGGCCTACTGCCAGCATATTGGGGTG
  GAGTTCATGTTCATCAATGACCTGGAGCAGTGCCAGTGGATCCGGCAGAAGTTTGAGACC
  CCTGGGATCATGCAGTTCACAAATGAGGAGAAACGGACCCTGCTGGCCAGGCTTGTGCGG
  TCCACCAGGTTTGAGGAGTTCCTACAGCGGAAGTGGTCCTCTGAGAAGCGCTTTGGTCTA
  GAAGGCTGCGAGGTACTGATCCCTGCCCTCAAGACCATCATTGACAAGTCTAGTGAGAAT
  GGCGTGGACTACGTGATCATGGGCATGCCACACAGAGGGCGGCTGAACGTGCTTGCAAAT
  GTCATCAGGAAGGAGCTGGAACAGATCTTCTGTCAATTCGATTCAAAGCTGGAGGCAGCT
  GATGAGGGCTCCGGAGATGTGAAGTACCACCTGGGCATGTATCACCGCAGGATCAATCGT
  GTCACCGACAGGAACATTACCTTGTCCTTGGTGGCCAACCCTTCCCACCTTGAGGCCGCT
  GACCCCGTGGTGATGGGCAAGACCAAAGCCGAACAGTTTTACTGTGGCGACACTGAAGGG
  AAAAAGGTCATGTCCATCCTGTTGCATGGGGATGCTGCATTTGCTGGCCAGGGCATTGTG
  TACGAGACCTTCCACCTCAGCGACCTGCCATCCTACACAACTCATGGCACCGTGCACGTG
  GTCGTCAACAACCAGATCGGCTTCACCACCGACCCTCGGATGGCCCGCTCCTCCCCCTAC
  CCCACTGACGTGGCCCGAGTGGTGAATGCCCCCATTTTCCACGTGAACTCAGATGACCCC
  GAGGCTGTCATGTACGTGTGCAAAGTGGCGGCCGAGTGGAGGAGCACCTTCCACAAGGAC
  GTGGTTGTCGATTTGGTGTGTTACCGGCGCAACGGCCACAACGAGATGGATGAGCCCATG
  TTCACGCAGCCGCTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTGTTACAGAAGTAC
  GCTGAGCTGCTGGTGTCGCAGGGTGTGGTCAACCAGCCTGAGTATGAGGAGGAAATTTCC
  AAGTATGATAAGATCTGTGAGGAAGCTTTTGCCAGATCTAAAGATGAGAAGATCTTGCAC
  ATTAAGCACTGGCTGGACTCTCCCTGGCCTGGCTTCTTCACCCTGGACGGGCAGCCCAGG
  AGCATGTCCTGCCCCTCCACGGGTCTGACGGAGGATATTCTGACACACATCGGGAATGTG
  GCTAGTTCTGTGCCTGTGGAAAACTTTACTATTCATGGAGGGCTGAGCCGGATCTTGAAG
  ACTCGTGGGGAAATGGTGAAGAACCGGACTGTGGACTGGGCTCTAGCGGAGTACATGGCG
  TTTGGCTCGCTCCTGAAGGAGGGCATCCACATTCGGCTGAGCGGCCAGGACGTGGAGCGG
  GGCACATTCAGCCACCGCCACCATGTGCTCCATGACCAGAATGTGGACAAGAGAACCTGC
  ATCCCCATGAACCATCTCTGGCCCAATCAGGCCCCCTATACTGTGTGCAACAGCTCACTG
  TCTGAGTACGGCGTGCTGGGCTTTGAGCTGGGCTTCGCCATGGCCAGTCCTAATGCCCTG
  GTCCTCTGGGAAGCCCAATTTGGTGACTTCCACAACACGGCCCAGTGTATCATCGACCAG
  TTCATCTGCCCGGGACAAGCCAAGTGGGTGCGGCAGAATGGCATCGTGTTGCTGCTGCCC
  CATGGCATGGAGGGCATGGGTCCAGAACATTCCTCCGCCCGCCCAGAGCGGTTCTTGCAG
  ATGTGCAACGATGACCCAGATGTCCTGCCAGACCTTAAAGAAGCCAACTTCGACATCAAT
  CAGCTATATGACTGCAATTGGGTTGTTGTCAACTGCTCCACTCCTGGCAACTTCTTCCAC
  GTGCTACGACGCCAGATCCTGCTGCCATTCCGGAAGCCGTTAATTATCTTCACCCCCAAA
  TCCCTGTTGCGCCACCCCGAGGCCAGATCCAGCTTTGATGAGATGCTTCCAGGAACCCAC
  TTCCAGCGGGTGATCCCAGAAGATGGCCCTGCAGCTCAGAACCCAGAAAATGTCAAAAGG
  CTTCTCTTCTGCACCGGCAAAGTGTATTATGACCTCACCCGGGAGCGCAAAGCACGCGAC
  ATGGTGGGGCAGGTGGCCATCACAAGGATTGAGCAGCTGTCGCCATTCCCCTTTGACCTC
  CTGCTGAAGGAGGTGCAGAAGTACCCCAATGCTGAGCTGGCCTGGTGCCAGGAGGAGCAC
  AAGAACCAAGGCTACTATGACTACGTGAAGCCAAGACTTCGGACCACCATCAGCCGCGCC
  AAGCCCGTCTGGTATGCCGGCCGGGACCCAGCAGCTGCTCCAGCCACCGGCAACAAGAAG
  ACCCACCTGACGGAGCTGCAGCGCCTCCTGGACACGGCCTTCGACCTGGACGTCTTCAAG
  AACTTCTCGTAG
  
  Clone variation with respect to NM_002541.3
  2973 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002541 unedited
  TGTTCAGNNCATATTTNGTNAATNACGNAACTTCACTTATNNAGGGNNCGGCNNCGCGNA
  ATTNNCGGCACGAAGGGCTTGNAGCCGGNANAAACAGGCAGTTGTGAAAAAACTTCAGGA
  CAAAAATGTTTCATTTAAGGACTTGTGCTGCTAAGTTGAGGCCATTGACGGCTTCCCAGA
  CTGTTAAGACATTTTCACAAAACAGACCAGCAGCAGCTAGGACATTTCAACAGATTCGGT
  GCTATTCTGCACCTGTTGCTGCTGAGCCCTTTCTCAGTGGGACTAGTTCGAACTATGTGG
  AGGAGATGTACTGTGCTTGGCTGGAAAACCCCAAAAGTGTACATAAGTCATGGGACATTT
  TTTTTCGCAACACGAATGCCGGAGCCCCACCGGGCACTGCCTACCAGAGTCCCCTTCCCC
  TGAGCCGAGGCTCCCTGGCTGCTGTGGCCCATGCACAGTCCCTGGTAGAAGCACAGCCCA
  ACGTGGACAAGCTCGTGGAGGACCACCTGGCAGTGCAGTCGCTCATCAGGGCATATCAGA
  TACGAGGGCACCATGTAGCACAGCTGGACCCCCTGNGGATTTTGGATGCTGATCTGGACT
  CCTCCGTGCCCGCTGACATTATCTCATCCACAGACAAACTTGGGTTCTATGGCCTGGATG
  AGTCTGACCTCGACAGGTCTTCCACTTGCCCACCACCACTTTCATCGGNGGACAGGAATC
  AGCACTTNCTCTGCGGGAGATCATCCGTCGGCTGGAGATGGCCTACTGCCAGCATATTGG
  GGTGGAGTTCATGTTCATCAATGACCTGNAGCAGTGCCAGTGGGATCCGCAGAAGTTNGA
  GACCCCTGGGATCATGCANGTCACAAATGAGGAGAAACGGACCCTGCTGGCCAAGCTTGT
  GCGGTCCACCCAGTTGAGGAGTTCCTACAACGGAAGTGTCCTCTGAGAACCGCTTTGTCT
  AAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002541 unedited
  TACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TCATGCCCACGGATATAACTTAGTTTATGGGTCAAGCCAATGCAATGCAAAGTTGTTTCT
  GTTCCTTTTTTTTTTTTTTTAACCAAAATAAATAAATCAAATCTGTCCCTGTGAAGGTCA
  AAGTGATAAATGAGGGGCAGCTGGCCCCTTGGCCATGGCCTGGCCCCTCACCCCCAGCCC
  CTCCTTTCCCTTTTTTGACCTGCTTTGGCACAGCAAAAAAAATGATGCATCAAAAAGTCT
  CCATTGGGTTAAAGGAAAAAAAAAAATTGGTTGGGGTGGGGAGGGATTCCAAAAAAGGAG
  GTGGAGGGTTAACAGGACCAAGACAGAGCAGCCAGTGCCCCGGCGGTGGCTACCTGAGCG
  GGGCTTCCTGGCTACACACAAACTCTGTGGGCTCCAGGTGCTGCCTGGGCCCAGGAGGGG
  TGGGGTGGGGCATGCTTTGGGCCCCACACACCGCCCTGGAACCCTTCCAAGGAACAGCCA
  AATAATGGGACACCTGCAAAGACTGAAGCCTGCCCCTTTTTTGTGCCAGTTCAGGGCCAA
  GGTTTTTTTTCCTGGCCAGCTGCCCCCGGGTAATCCCTCATTTCACGCCCCCGGGTGGGG
  GCCCCACCCAAAAAACCCTCTGCCCGCCCTGGGTTAGATCCGAACTCCAGGCTCCCCCTT
  AAAAATTCTAAGGGCTCAGGCTCAAAATGAAACCTTTCCTAGTTCTAAAATCCAGGGACC
  CTGTGCCTTGGTTGGGGGACAGCCGGAACCCCCCCCAACAGGGCCAATTTCCAATTCCAA
  ATGGGTGAAACTGGTGGGGAGGGTTTTTTNCANATACTANACCTCCCCACAGAAATTCCT
  TAGAGGTTTATTTTGCCCTTCGGGCTGCCCCAGCGNCTGGAGAAAATGTNCAGATTATAT
  AAACCCGGNCCCTGGTGCCCCGGGGGAATCAAATATTGTGGGGGTGGACCAGTCCCTCCC
  TTTCGCGCTGAGGGGGTTTCTCCCCCTGTAGACGTCGTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002541
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002541.2, NP_002532.2
• RefSeq Size: 4257 bp
• RefSeq ORF: 3072 bp
• Locus ID: 4967
• UniProt ID: Q02218
• Cytogenetics: 7p13
• Domains: E1_dehydrog, transket_pyr
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways, Tryptophan metabolism
• Gene Summary: This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).