PASK (NM_015148) Human Untagged Clone

CAT#: SC127392

PASK (untagged)-Human PAS domain containing serine/threonine kinase (PASK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PASK
• Synonyms: PASKIN; STK37
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015148, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGACGGGGGCTTAACAGCCTTTGAAGAGGACCAGAGATGCCTTTCCCAGAGCCTCCCCTTGCCAG
  TGTCAGCAGAGGGCCCAGCTGCACAGACCACTGCTGAGCCCAGCAGGTCGTTTTCCTCAGCCCACAGACA
  CCTGAGCAGAAGGAATGGGCTTTCCAGACTCTGCCAGAGCAGGACAGCGCTCTCTGAAGACAGATGGAGC
  TCCTATTGTCTATCATCACTGGCTGCCCAGAATATTTGTACAAGTAAACTGCACTGCCCTGCTGCCCCTG
  AGCACACGGACCCGTCCGAACCGCGGGGCAGTGTGTCCTGCTGCTCCCTGCTGCGGGGACTGTCCTCAGG
  GTGGTCCTCACCTCTGCTTCCGGCCCCTGTGTGCAACCCTAACAAGGCCATCTTCACGGTGGATGCCAAG
  ACCACAGAGATCCTGGTTGCTAACGACAAAGCTTGCGGGCTCCTGGGGTACAGCAGCCAGGACCTGATTG
  GCCAGAAGCTCACGCAGTTCTTTCTGAGGTCAGATTCTGATGTGGTGGAGGCCCTCAGCGAGGAGCACAT
  GGAGGCCGACGGCCACGCTGCGGTGGTGTTTGGCACGGTGGTGGACATCATCAGCCGTAGTGGGGAGAAG
  ATTCCAGTGTCTGTGTGGATGAAGAGGATGCGGCAGGAGCGCCGCCTATGCTGCGTGGTGGTCCTGGAGC
  CCGTGGAGAGGGTCTCGACCTGGGTCGCTTTCCAGAGCGATGGCACCGTCACGTCATGTGACAGTCTCTT
  TGCTCATCTTCACGGGTACGTGTCTGGGGAGGACGTGGCTGGGCAGCATATCACAGACCTGATCCCTTCT
  GTGCAGCTCCCTCCTTCTGGCCAGCACATCCCAAAGAATCTCAAGATTCAGAGGTCTGTTGGAAGAGCCA
  GGGACGGTACCACCTTCCCTCTGAGCTTAAAGCTGAAATCCCAACCCAGCAGCGAGGAGGCGACCACCGG
  TGAGGCGGCCCCTGTGAGCGGCTACCGGGCATCTGTCTGGGTGTTCTGCACCATCAGTGGCCTCATCACC
  CTCCTGCCGGATGGGACCATCCACGGCATCAACCACAGCTTCGCGCTGACACTGTTTGGTTACGGAAAGA
  CGGAGCTCCTGGGCAAGAATATCACTTTCCTGATTCCTGGTTTCTACAGCTACATGGACCTTGCGTACAA
  CAGCTCATTACAGCTCCCAGACCTGGCCAGCTGCCTGGACGTCGGCAATGAGAGTGGGTGTGGGGAGAGA
  ACCTTGGACCCGTGGCAGGGCCAGGACCCAGCTGAGGGGGGCCAGGATCCAAGGATTAATGTCGTGCTTG
  CTGGTGGCCACGTTGTGCCCCGAGATGAGATCCGGAAGCTGATGGAAAGCCAAGACATCTTCACCGGGAC
  TCAGACTGAGCTGATTGCTGGAGGCCAGCTCCTTTCCTGCCTCTCACCTCAGCCTGCTCCAGGGGTGGAC
  AATGTCCCAGAAGGAAGCCTGCCAGTGCACGGTGAACAGGCGCTGCCCAAGGACCAGCAAATCACTGCCT
  TGGGGAGAGAGGAACCTGTGGCAATAGAGAGCCCCGGACAGGATCTTCTGGGAGAAAGCAGGTCTGAACC
  AGTGGATGTGAAGCCATTTGCTTCCTGCGAAGATTCTGAAGCTCCAGTCCCAGCTGAGGATGGGGGCAGT
  GATGCTGGCATGTGTGGCCTGTGTCAGAAGGCCCAGCTAGAGCGGATGGGAGTCAGTGGTCCCAGCGGTT
  CAGACCTTTGGGCTGGGGCTGCCGTGGCCAAGCCCCAGGCCAAGGGTCAGCTGGCGGGGGGCAGCCTCCT
  GATGCACTGCCCTTGCTATGGGAGTGAATGGGGCTTGTGGTGGCGAAGCCAGGACTTGGCCCCCAGCCCC
  TCTGGGATGGCAGGCCTCTCGTTTGGGACACCTACTCTAGATGAGCCGTGGCTGGGAGTGGAAAACGACC
  GAGAAGAGCTGCAGACCTGCTTGATTAAGGAGCAGCTGTCCCAGTTGAGCCTTGCAGGAGCCCTGGATGT
  CCCCCACGCCGAACTCGTTCCGACAGAGTGCCAGGCTGTCACCGCTCCTGTGTCGTCCTGCGATCTGGGA
  GGCAGAGACCTGTGCGGTGGCTGCACGGGCAGCTCCTCAGCCTGCTATGCCTTGGCCACGGACCTCCCTG
  GGGGCCTGGAAGCAGTGGAGGCCCAGGAGGTTGATGTGAATTCGTTTTCCTGGAACCTCAAGGAACTCTT
  TTTCAGTGACCAGACAGACCAAACGTCATCAAATTGTTCCTGTGCTACGTCTGAACTCAGAGAGACACCC
  TCTTCCTTGGCAGTGGGCTCCGATCCAGATGTAGGCAGTCTCCAGGAACAGGGGTCGTGTGTCCTGGATG
  ACAGGGAGCTGTTACTACTGACCGGCACCTGTGTTGACCTTGGCCAAGGCCGACGGTTCCGGGAGAGCTG
  TGTGGGACATGATCCAACAGAACCGCTTGAGGTTTGTTTGGTGTCCTCTGAGCATTATGCAGCAAGCGAC
  AGAGAAAGCCCAGGACACGTTCCTTCCACGTTGGATGCTGGCCCTGAGGACACGTGCCCATCAGCAGAGG
  AGCCAAGGCTGAACGTCCAGGTCACCTCCACGCCCGTGATCGTGATGCGCGGGGCTGCTGGCCTGCAGCG
  GGAGATCCAGGAGGGTGCCTACTCCGGGAGCTGCTACCATCGAGATGGCTTACGGCTGAGTATACAGTTT
  GAGGTGAGGCGGGTGGAGCTCCAGGGCCCCACACCTCTGTTCTGCTGCTGGCTGGTGAAAGACCTCCTCC
  ACAGCCAACGCGACTCAGCCGCCAGGACCCGCCTGTTCCTTGCCAGCCTGCCCGGCTCCACCCACTCTAC
  CGCTGCTGAGCTCACCGGACCCAGCCTGGTGGAAGTGCTCAGAGCCAGACCCTGGTTTGAGGAGCCCCCC
  AAGGCTGTGGAACTGGAGGGGTTGGCGGCCTGTGAGGGCGAGTACTCCCAAAAGTACAGTACCATGAGCC
  CGCTGGGCAGTGGGGCCTTCGGCTTCGTGTGGACTGCTGTGGACAAGGAAAAAAACAAGGAGGTGGTGGT
  GAAGTTTATTAAGAAGGAGAAGGTCTTGGAGGATTGTTGGATTGAGGATCCCAAACTTGGGAAAGTTACT
  TTAGAGATCGCAATTCTATCCAGGGTGGAGCACGCCAATATCATCAAGGTATTGGATATATTTGAAAACC
  AAGGGTTCTTCCAGCTTGTGATGGAGAAGCACGGCTCCGGCCTAGACCTCTTCGCTTTCATCGACCGCCA
  CCCCAGGCTGGATGAGCCCCTGGCGAGCTACATCTTCCGACAACTAGTGTCAGCAGTGGGATACCTGCGC
  TTGAAGGACATCATCCACCGTGACATCAAGGATGAGAACATCGTGATCGCCGAGGACTTCACAATCAAGC
  TGATAGACTTTGGCTCGGCCGCCTACTTGGAAAGGGGAAAATTATTTTATACTTTTTGTGGGACCATCGA
  GTACTGTGCACCGGAAGTTCTCATGGGGAATCCCTACAGAGGGCCGGAGCTGGAGATGTGGTCTCTGGGA
  GTCACTCTGTACACGCTGGTCTTTGAGGAGAACCCCTTCTGTGAGCTGGAGGAGACCGTGGAGGCTGCCA
  TACACCCGCCATACCTGGTGTCCAAAGAACTCATGAGCCTTGTGTCTGGGCTGCTGCAGCCAGTCCCTGA
  GAGACGCACCACCTTGGAGAAGCTGGTGACAGACCCGTGGGTAACACAGCCTGTGAATCTTGCTGACTAT
  ACATGGGAAGAGGTGTTTCGAGTAAACAAGCCAGAAAGTGGAGTTCTGTCCGCTGCGAGCCTGGAGATGG
  GGAACAGGAGCCTGAGTGATGTGGCCCAGGCTCAGGAGCTTTGTGGGGGCCCCGTTCCAGGCGAGGCTCC
  TAATGGCCAAGGCTGTTTGCATCCCGGGGATCCCCGTCTGCTGACCAGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015148 unedited
  CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGTGCGATTGGTCGGTGGGATA
  AAAGCGCGGAAGGGCTCCTTTCCGTGGTGTGTAGCCGGCTTGGCGTGACCCTCGCCTGAT
  CCAGTTGTTAGAGTTGGAAGCTTGGCAGTTGGCCTCCCTTCTTCCCATGGAGGACGGGGG
  CTTAACAGCCTTTGAAGAGGACCAGAGATGCCTTTCCCAGAGCCTCCCCTTGCCAGTGTC
  AGCAGAGGGCCCAGCTGCCAGACACTGCTGACCCACAGGTCGTTTCTCACCCACAACCCT
  GACAGAAGATGGCTTTCAACTCTGCAGACAGGCGGCGCTTTGAAACAGATGGACTCTATT
  GCTATCATCACTGGCTGCCAGAATATTTGTCAAGTAACTGCCTGCCTGCTGCCCTGNCAC
  ACGGACCGTCCGAACCGCGGCAGTGTGTCCTGCTGTCCCTGTGCGGGACTGTCTCANGGT
  GTCCTCACCTCTGTTCCGGCNCTGTGTCAACCCTAACAGGCATCTTCCGGTGATGCCAGA
  CACAGAATCCTGTTGCTAACACAAGCTTGCGGCTCCTGGGTACAGCAGCCGGACCTGATT
  GCCAGAACTCACGCAGTTCTTCTGAGGTCNATTCTGATGTGGTGAGGCCCTCAGCGAGGA
  CACATGGAGGCCAGGCCACGCTGCGTGGTGTTGGCACGTGTGACATATCACCGTATGGGA
  GAGATCAGTGCTGTTGATAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015148 unedited
  CCAGAGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACTAAC
  AAAGGTGCTTTAATTTGAAAAGCATTTGAGGAAATAAATTAATGAAATAGTCCGGCCATT
  TGACTAACCAGTTCTACAAATTTCACATATCCGTCACTCAGATGAGCATATACCAAGTCA
  GAGGAAACAAAACATGCACATATACAAAAGTAGAAAGAGAAAACACTCATGCCTTCAGAA
  GTTCACAAATTAAAAGCCCTTTAATAATATAAAACAGTTGAACACTGGAATGTTTTTTTC
  TAGGTCATGAAAAAAGTGAATTCCAAATCTATGTAATAAATCTAAAATAATACAGCATCA
  CTGTCTTCTGTTCTGGTGTTTATCAAACCTGCACATGAGTTTTTAGAAGGTGAATTGGGG
  ATGCTTCAGAATGTATTTTCTCCAAACAGATGGAGCCTGAAAACTGTGTGATTTTCCAAA
  CCAAGTGGAGAAAAGCAGGAAGAAATTGGTGTTTAGCTGGTCAGCAGACGGNGATCCCCG
  GGATGCAAACAGCCTTGGCCATTAGGAGCCTCGCCTGGAACGGGGCCNCCACAAAGCTCC
  TGAGCCTGGGCCACATCACTCAGGCTCCTGTTCCCCATCTCCAGGCTCGCAGCGGACAGA
  ACTCCACTTTCTGGCTTGTTTAACTCAAACACCTTCTTCCATTGTTAGTCAGCAAGAATC
  ACAGGGCTGGGTTACCCACGGGGTCGGCCACCAGCTTTCTCCAGGGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_015148
• Insert Size: 5100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015148.2, NP_055963.2
• RefSeq Size: 4530 bp
• RefSeq ORF: 3972 bp
• Locus ID: 23178
• UniProt ID: Q96RG2
• Cytogenetics: 2q37.3
• Domains: S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Gene Summary: This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, but maintains the reading frame, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which is shorter than isoform 1.